H2O2로 산화적 스트레스가 유도된 HaCaT Keratinocytes에서 Genistein의 항산화 활성
Anti-oxidant Function of Genistein against H2O2-induced Oxidative Stress in HaCaT Keratinocytes
2
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
The nutri-cosmetics was defined as a nutraceuticals which can exert the function, beauty and the structure of the skin. Genistein has been reported to possess potent pro-apoptotic, anti-inflammatory, and anti-oxidant properties. However, the physiological role and its underlying molecular mechanisms of genistein in H2O2-treated HaCaT keratinocytes are unknown. In this study, we examined the cellular anti-oxidant activity of genistein against H2O2-induced oxidative stress in HaCaT keratinocytes. The cellular anti-oxidant activity of genistein was assessed in HaCaT cells as judged by the 2’7’-dichlorofluorescein-diacetate (DCFH-DA)-based assay and image analysis using fluorescence microscopy. Genistein dose-dependently and effectively ameliorate the H2O2-induced oxidative stress of HaCaT keratinocytes. We further revealed that the cellular anti-oxidant activity of genistein mediated by activating of nuclear factor erythroid-2-related factor 2 (Nrf2), the central transcriptional factor for expression of phase II detoxifying enzymes such as heme oxygenase-1 (HO-1). Taken together, our results indicate that the activation of Nrf2 signaling is required for cellular anti-oxidant activity of genistein. These findings provide the scientific evidences for the development of Nrf2 targeted nutricosmetics.
Abstract
The nutri-cosmetics was defined as a nutraceuticals which can exert the function, beauty and the structure of the skin. Genistein has been reported to possess potent pro-apoptotic, anti-inflammatory, and anti-oxidant properties. However, the physiological role and its underlying molecular mechanisms of genistein in H2O2-treated HaCaT keratinocytes are unknown. In this study, we examined the cellular anti-oxidant activity of genistein against H2O2-induced oxidative stress in HaCaT keratinocytes. The cellular anti-oxidant activity of genistein was assessed in HaCaT cells as judged by the 2’7’-dichlorofluorescein-diacetate (DCFH-DA)-based assay and image analysis using fluorescence microscopy. Genistein dose-dependently and effectively ameliorate the H2O2-induced oxidative stress of HaCaT keratinocytes. We further revealed that the cellular anti-oxidant activity of genistein mediated by activating of nuclear factor erythroid-2-related factor 2 (Nrf2), the central transcriptional factor for expression of phase II detoxifying enzymes such as heme oxygenase-1 (HO-1). Taken together, our results indicate that the activation of Nrf2 signaling is required for cellular anti-oxidant activity of genistein. These findings provide the scientific evidences for the development of Nrf2 targeted nutricosmetics.
참고문헌 (24)
- 김낙인, "피부 건강 및 미용에 있어 비타민과 미네랄의 역할", 식품과학과, 38, pp.16~25 (2005)
- 김은아, "식물생리활성영양소와 미용기능식품", 식품과학과 산업, 40, pp.3~9 (2007)
- 김형우, "포공영 추출물의 항산화 효과 및 피부 각질세포 보호효과", 대한본초학회지, 24(3), pp.103~108 (2009), 대한본초학회
- 문갑순, "측정방법에 따른 콩 성분의 항산화효과 비교", 한국콩연구회지, 20, pp.28~36 (2003)
- 이미경, "Genistein과 Daidzein 급여가 제2형 당뇨동물모델의 적혈구와 조직 중의 항산화방어계에 미치는 영향", 한국식품영양과학회지, 35(9), pp.1159~1165 (2006), 한국식품영양과학회
- 박진영, "Vitamin C를 미세입자로 분사에 의한 피부개선 효과", 대한피부미용학회지, 8(2), pp.253~262 (2010), 대한피부미용학회
- 윤미영, "샌달우드 오일의 항산화 및 항염증에 관한 효과", 대한피부미용학회지, 7(3), pp.263~274 (2009), 대한피부미용학회
- 이선영, "이질풀 추출물의 항산화 효능에 관한 연구", 대한화장품학회지, 37(1), pp.61~66 (2011), 대한화장품학회
- 허선정, "목단피 에탄올 추출물의 B16F10 멜라닌 생성 세포의 저해 작용과 항산화 활성", 대한피부미용학회지, 8(1), pp.1~9 (2010), 대한피부미용학회
- Abhinav KJ, "Phosphorylation of tyrosine 568 controls nuclear export of Nrf2", J. Biol. Chem, 281, pp.12132~12142 (2006)
- Choi YH, "Genistein-induced G2", M arrest is associated with the inhibition of cyclin B1 and the induction of p21 in human breast carcinoma cells, 1998, pp.13, 391~396
- Fang F, "Luteolin inhibits apoptosis and improves cardiomyocyte contractile function through the PI3K", Akt pathway in stimulated ischemia, Pharmacol, pp.2011, 88[DOI]
- Lautraite S, "Optimisation of cell-based assays for medium throughput screening of oxidative stress", Toxicol. In Vitro, 17, pp.207~220 (2003)
- Majid S, "Regulation of minichromosime maintenance gene family by microRNA-1296 and genistein in prostate cancer", Cancer Res, 70, pp.2809~2818 (2010)
- Matsukawa Y, "Genistein arrests cell cycle progression at G2-M", Cancer Res, 53, pp.1328~1331 (1993)
- Paine A, "Signaling to heme oxygenase-1 and its antiinflammatory therapeutic potential", Biochem. Pharmacol, 80, pp.1895~1903 (2010)
- 이승민, "Effects of genistein on early-stage cutaneous wound healing", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 410(3), pp.514~519, ACADEMIC PRESS inC ELSEVIER SCIENCE
- Park SE, "Kaempferol acts through mitogen-activated protein kinases and protein kinase B", AKT to elicit protection in a model of neuroinflammation in BV2 microglial cells, 2011, pp.164, 1008~1025[DOI]
- Perry G, "How important is oxidative damage? Lessons from Alzheimer’s disease", Free Radic. Biol. Med, 28, pp.831~834 (2000)
- Song T, "Soy isoflavone anlysis: quality control and a new internal standard", Am. J. Clin. Nutr, 68, pp.1474~1479 (1998)
- Trakshel GM, "Purification and characterization of the major constitutive form of testicular heme oxygenase. The noninducible isoform", J. Biol. Chem, 261, pp.11131~11137 (1986)
- Upadhyay S, "Differential sensitivity of normal and malignant breast epithelial cells to genistein is partly mediated by p21WAF1", Clin. Cancer. Res, 7, pp.1782~1789 (2001)
- Wang SH, "Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen", J. Agric. Food Chem, 48, pp.5677~5684 (2000)
- Wang YN, "Genistein protects against UVB-induced senescence-like characteristics in human dermal fibroblast by p66Shc downregulation", J. Dermatol. Sci, 58, pp.19~27 (2010)