과산화수소(H2O2)에 의해 유도된 인간 각질형성세포주 손상에 대한 키네틴(Kinetin)의 항산화 효과
Antioxidant Effects of Kinetin on HaCaT Human Keratinocytes Damaged by Hydrogen Peroxide (H2O2)
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초록
This study aimed to verify functions of kinetin as a raw material for cosmetics products in skin through the cell efficacy test. Kinetin, plant cytokinins, regulates plant growth. It has been reported that kinetin has antioxidant effects, synthesis promotion of DNA, and senescence delay on dermal fibroblast in cellular function. But there is no study reported in effects of kinetin on keratinocytes. Hydrogen peroxide (H2O2) is a species of reactive oxygen, affects to fate of cells in growth, senescence, and extinction. H2O2 is unstable property because of free radicals. As unstable property, H2O2 reacts strong with other molecules and damage to cells and DNA. To verify the efficacy of kinetin on HaCaT human keratinocyte cells, the cell viability essay, and reactive oxygen species (ROS) assay were performed. As kinetin with concentrations of 200 μM and above were toxic in HaCaT cells, this study used kinetin with concentrations of less than 200 μM. Cell viability assay showed that kinetin efficiently rescued HaCaT cells from H2O2-induced oxidative stress damages in a concentration-dependent manner. Furthermore, flow cytometry analysis revealed that kinetin decreased the intracellular ROS levels in H2O2-treated HaCaT cells. These data indicated that kinetin can be used as a raw effective natual material for for functional cosmetic products which can reduce facial wrinkles, came from ROS, and improve the condition and function of skin.
Abstract
This study aimed to verify functions of kinetin as a raw material for cosmetics products in skin through the cell efficacy test. Kinetin, plant cytokinins, regulates plant growth. It has been reported that kinetin has antioxidant effects, synthesis promotion of DNA, and senescence delay on dermal fibroblast in cellular function. But there is no study reported in effects of kinetin on keratinocytes. Hydrogen peroxide (H2O2) is a species of reactive oxygen, affects to fate of cells in growth, senescence, and extinction. H2O2 is unstable property because of free radicals. As unstable property, H2O2 reacts strong with other molecules and damage to cells and DNA. To verify the efficacy of kinetin on HaCaT human keratinocyte cells, the cell viability essay, and reactive oxygen species (ROS) assay were performed. As kinetin with concentrations of 200 μM and above were toxic in HaCaT cells, this study used kinetin with concentrations of less than 200 μM. Cell viability assay showed that kinetin efficiently rescued HaCaT cells from H2O2-induced oxidative stress damages in a concentration-dependent manner. Furthermore, flow cytometry analysis revealed that kinetin decreased the intracellular ROS levels in H2O2-treated HaCaT cells. These data indicated that kinetin can be used as a raw effective natual material for for functional cosmetic products which can reduce facial wrinkles, came from ROS, and improve the condition and function of skin.
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