Sinapic acid의 인간각질형성세포에서 UVB의 손상에 대한 항산화 및 항염증 효과
Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes
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목적: 본 연구는 인간각질형성세포(HaCaT)에서 자외선(UV) 손상에 대한 항산화 및 항염증 효과를 확인하여 피부노화 예방 화장품 소재로서 sinapic acid의 가능성을 확인하고자 한다. 방법: Sinapic acid를 농도별로 HaCaT에 처리 후 UVB로 산화적 스트레스를 유도하였다. UVB에 대한 sinapic acid의 세포보호효과를 확인하기 위하여 water-soluble tetrazolium salt (WST-1)를 이용하였으 며, UVB에 의하여 생성된 활성산소종(reactive oxygen species, ROS)의 농도변화는 2’,7’-dichlorofluorescin diacetate (DCF-DA)를 사용하여 측정하였다. 항산화 및 염증과 관련된 유전자 발현은 quantitative real-time polymerase chain reaction (qRT-PCR)을 통해 확인하였다. 결과: Sinapic acid는 UVB에 의한 세포독성으로부터 세포를 보호하고, ROS의 생성을 감소시킨다. 항산화와 관련한 유전자인 superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), nuclear factor (erythroid-derived 2)-like 2 (NRF2) 및 heme oxygenase 1 (HO1)의 발현을 증가하게 하였고, 지질 과산화는 농도 의존적으로 감소하였다. 또한, 염증과 관련이 있는 cyclooxygenase 2 (COX2), interleukin 6 (IL6)와 interleukin 8 (IL8)의 유전자 발현은 농도 의존적으로 감소하였다. 결론: 본 연구를 통하여 sinapic acid가 UVB에 대하여 항산화 및 항염증의 효과가 있음을 확인하였으며 이는 노화예방 화장품 성분 으로서의 가능성이 있을 것으로 사료된다.
Abstract
Purpose: The purpose of the present study was to explore the possibility of using sinapic acid as a skin anti-aging cosmetic by demonstrating its antioxidant and anti-inflammatory effects in keratinocytes damaged by ultraviolet (UV) irradiation. Methods: The effects of sinapic acid against oxidative stress were examined in a UVB-induced aging model of human keratinocyte HaCaT cells. The water-soluble tetrazolium salt (WST-1) assay was employed to examine protective effects against UVB. Changes in the concentration of reactive oxygen species (ROS) produced by UVB were measured using 2’,7’-dichlorofluorescin diacetate (DCF-DA). Expression of genes associated with anti-oxidant and anti-inflammatory effects were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Sinapic acid protected cells from UVB-induced cytotoxicity and reduced ROS produced by UVB irradiation. Expression of genes associated with antioxidant effects including, superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), nuclear factor (erythroid-derived 2)-like 2 (NRF2), and heme oxygenase 1 (HO1), increased and lipid peroxidation decreased in a dose-dependent manner. The expression of cyclooxygenase 2 (COX2), interleukin 6 (IL6), and interleukin 8 (IL8) genes, which are associated with inflammation, also decreased in a dose-dependent manner. Conclusion: These results suggest that sinapic acid has potential as an anti-aging cosmetic ingredient with anti-oxidant, anti-inflammatory properties against UVB irradiation.
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