Fisetin의 항산화 활성 및 미용기능식품 소재로서의 가능성 고찰
Anti-oxidative Function of Fisetin and Its Potential as an Anti-oxidant Nutri-cosmetics
2
조회
0
다운로드
8
인용
0
참고문헌
키워드
초록
The term of nutri-cosmetics refers to nutritional supplements which can support the function and the structure of the skin. The fisetin is a flavonol which belongs to the flavonoid group of polyphenols. Although the anti-carcinogenic property of fisetin have been widely studied, anti-oxidative function of fisetin in HaCaT keratinocytes is unknown. In this study, we examined the in vitro chemical and cellular anti-oxidant activities of fisetin in HaCaT keratinocytes and we further discussed its potential as an anti-oxidant nutricosmetics. We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and cellular anti-oxidant activity of fisetin was investigated in H2O2-treated HaCaT keratinocytes as judged by the 2’7’-dichlorofluorescein-diacetate (DCFH-DA)-based assay. The fisetin effectively scavenged DPPH radicals (IC50 54 μM) when compared to the scavenging activities of L-ascorbic acid (IC50 87 μM) acid and trolox (IC5074 μM), well-known anti-oxidants. In addition, the fisetin significantly ameliorates H2O2-induced oxidative stress in HaCaT keratinocytes in dose-dependent manner. The fisetin showed more strong cellular anti-oxidant activity than those of L-ascorbic acid and trolox in H2O2-treated HaCaT keratinocytes. In agreement with result of the cellular anti-oxidant activity, we observed markedly suppressed H2O2-induced oxidative stress by the pretreatment of 100 μM fisetin under the fluorescence microscopy analysis. These results provide the scientific evidences for the development of skin targeted nutri-cosmetics which has anti-oxidant function.
Abstract
The term of nutri-cosmetics refers to nutritional supplements which can support the function and the structure of the skin. The fisetin is a flavonol which belongs to the flavonoid group of polyphenols. Although the anti-carcinogenic property of fisetin have been widely studied, anti-oxidative function of fisetin in HaCaT keratinocytes is unknown. In this study, we examined the in vitro chemical and cellular anti-oxidant activities of fisetin in HaCaT keratinocytes and we further discussed its potential as an anti-oxidant nutricosmetics. We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and cellular anti-oxidant activity of fisetin was investigated in H2O2-treated HaCaT keratinocytes as judged by the 2’7’-dichlorofluorescein-diacetate (DCFH-DA)-based assay. The fisetin effectively scavenged DPPH radicals (IC50 54 μM) when compared to the scavenging activities of L-ascorbic acid (IC50 87 μM) acid and trolox (IC5074 μM), well-known anti-oxidants. In addition, the fisetin significantly ameliorates H2O2-induced oxidative stress in HaCaT keratinocytes in dose-dependent manner. The fisetin showed more strong cellular anti-oxidant activity than those of L-ascorbic acid and trolox in H2O2-treated HaCaT keratinocytes. In agreement with result of the cellular anti-oxidant activity, we observed markedly suppressed H2O2-induced oxidative stress by the pretreatment of 100 μM fisetin under the fluorescence microscopy analysis. These results provide the scientific evidences for the development of skin targeted nutri-cosmetics which has anti-oxidant function.
참고문헌 (27)
- 김돈영, "NIH3T3 세포에서 UVB에 의한 세포고사에 미치는 옻 추출물과 fisetin의 효과", 생명과학회지, 15(1), pp.141~146 (2005), 한국생명과학회
- 김설미, "안면 피부관리와 항산화 비타민 섭취의 피부건강 상태 변화", 대한피부미용학회지, 7(4), pp.111~125 (2009), 대한피부미용학회
- 김세건, "3T3-L1 세포에 대한 옻나무 추출물의 지방축적 억제효과", 생약학회지, 41(1), pp.21~25 (2010), 한국생약학회
- 김형우, "포공영 추출물의 항산화 효과 및 피부 각질세포 보호효과", 대한본초학회지, 24(3), pp.103~108 (2009), 대한본초학회
- 박수남, "플라보노이드(flavonoid): 활성산소종에 의한 산화 방어제", 화학세계, 47, pp.46~47 (2007)
- 윤미영, "샌달우드 오일의 항산화 및 항염증에 관한 효과", 대한피부미용학회지, 7(3), pp.263~274 (2009), 대한피부미용학회
- 이상준, "미용식품의 국내외 시장동향", 식품과학과 산업, 38, pp.2~7 (2005)
- 이선영, "이질풀 추출물의 항산화 효능에 관한 연구", 대한화장품학회지, 37(1), pp.61~66 (2011), 대한화장품학회
- 박종군, "NIH3T3 세포에서 UVB에 의한 세포고사와 DNA 단사절단에 미치는 fisetin의 효과", 생명과학회지, 17(2), pp.64~69 (2007), 한국생명과학회
- 정은영, "제주도 토착 우뭇가사리의 항산화활성 및 HaCaT 세포 재생효과", 대한피부미용학회지, 8(4), pp.171~180 (2010), 대한피부미용학회
- 조윤희, "피부, 영양 그리고 건강기능식품", 식품과학과산업, 38, pp.8~15 (2005)
- 차재영, "감귤류 플라보노이드의 생리 기능 활성", 한국농화학회지, 44, pp.122~128 (2001)
- Ahmad N, "Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor κB in cancer cells versus normal cells", Arch. Biochem. Biophys, 376, pp.338~346 (2000)
- Arai Y, "Dietary intakes of flavonols, flavones and isoflavones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol concentration", J. Nutr, 130, pp.2243~2250 (2000)
- Chen HM, "Antioxidative properties of histidinecontaining peptides designed from peptiede fragments found in the digests of a soybean protein", J. Agric. Food Chem, 46, pp.49~53 (1998)
- Chen YC, "Wogonin and fisetin induction of apoptosis through activation of caspase 3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1", Arch. Toxicol, 76, pp.351~359 (2002)
- Higa S, "Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils", J. Allergy Clin. Immun, 111, pp.1299~1306 (2003)
- Huang D, "The chemistry behind antioxidant capacity assays", J. Agric. Food Chem, 53, pp.1841~1856 (2005)
- Kelly EH, "Flavonoid antioxidants: chemistry, metabolism and structureactivity relationships", J. Nutr. Biochem, 13, pp.572~584 (2002)
- Khan N, "Fisetin, a novel dietary flavonoid, causes apoptosis and cell cycle arrest in human prostate cancer LNCaP cells", Carcinogenesis, 29, pp.1049~1056 (2008)
- Kim GN, "Flavonol Content in the Water Extract of the Mulberry (Morus alba L.) Leaf and Their Antioxidant Capacities", Journal of Food Science, 76(6), pp.869~873 (2011), Blackwell Publishing Inc.
- Lautraite S, "Optimisation of cell-based assays for medium throughput screening of oxidative stress", Toxicol. In Vitro, 17, pp.207~220 (2003)
- Jae Kag Lim, "Antioxidant and Antimicrobial Activities of Various Solvent Fractions of Fine Ginseng Root", Food Science and Biotechnology, 18(2), pp.513~518 (2009), 한국식품과학회
- Maher P, "A comparison of the neurotrophic activities of the flavonoid fisetin and some of its derivatives", Free Radical Res, 40, pp.1105~1111 (2006)
- Chang-Ho Oh, "Effects of Heat Processing Time on Total Phenolic Content and Antioxidant Capacity of Ginseng Jung Kwa", Journal of Ginseng Research, 34(3), pp.198~204 (2010), 고려인삼학회
- Robert J, "Flavonoids: a review of probable mechanisms of action and potential applications", Am. J. Clin. Nutr, 74, pp.418~425 (2001)
- Yao K, "The flavonoid , fisetin, inhibits UV radiation-induced oxidative stress and the activation of NF-кB and MAPK signaling in human lens epithelial cells", Mol. Vis, 14, pp.1865~1871 (2008)