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화장품 소재 개발을 위한 amentoflavone의 항산화 및 항주름 효과 연구

Antioxidant and Antiwrinkle Effects of Amentoflavone for Cosmetic Materials Development

안성관|이송정
한국피부과학연구원 (Korea Institute of Dermatological Sciences)|2016.03|아시안뷰티화장품학술지 (Asian Journal of Beauty and Cosmetology)|제14권 제1호|pp. 66-76|DOI http://dx.doi.org/10.20402/ajbc.2016.0027

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키워드

항산화항주름抗氧化抗皱Amentoflavone인간진피섬유아세포人体真皮纤维细胞노화예방预防老化
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초록

목적: 본 연구는 항산화 및 항주름 효과검증을 통해 amentoflavone의 노화예방 화장품 소재로서의 발전가능성을 확인하는데 그 목적이 있다. 방법: amentoflavone의 항산화 및 항주름 효과를 확인하기 위해i n vitro antioxidant assays, quantitative real-time PCR, SA- β-galactosidase assay, DCF-DA assay를 수행하였다. 결과: amentoflavone은 0.2 mM의 H2O2 처리 시, 15 μM농도까지 농도 의존적으로 세포 생존율을 증가시켰으며, 양성 대조군인BHT와 유사한 항산화 효과를 나타냈다. 유전자 수준에서 대표적 항산화 효소인S OD1 mRNA와 CAT mRNA의 발현을 농도 의존적으로 증가시켰다. 콜라겐 분해와 관련된 MMP1 mRNA와 MMP3 mRNA의 발현은 농도 의존적으로 감소시켰으나 콜라겐 합성과관련된 COL1A1 은 농도 의존적으로 증가시켰다. 또한 0.2 mM의 H2O2 에 의해 유발된 진피섬유아세포의 노화를 농도 의존적으로감소시키는 것으로 나타났다. 결론: 이상의 결과들을 통해, amentoflavone이 농도 의존적으로 H2O2 에 의해 손상된 인간진피섬유아세포에 대한 항산화, 항주름효과가 있음이 확인되었다. 그러므로 amentoflavone은 노화예방 효능을 가진 천연유래 화장품 원료로서 활용가능하리라고 사료된다.

Abstract

Purpose: This study aimed to investigate the potential of amentoflavone as an antiaging material for cosmetics through examining antioxidant and antiwrinkle effects. Methods: To check the antioxidant and antiwrinkle effects of amentoflavone, we performed various experiments such as in vitro antioxidant assays, quantitative real-time PCR, SA-β-galactosidase assay, and DCF-DA assay. Results: We confirmed that the cell viability of amentoflavone is increased in cytotoxic condition. Amentoflavone also had an antioxidant effect like BHT. The expression in mRNA level of antioxidant enzymes such as SOD1 and CAT was increased in a dose-dependent manner. In case of wrinkle, the expression in mRNA level of MMP1 and MMP3 was decreased but the expression of COL1A1 was dose-dependently increased. Also, amentoflavone markedly reduced the expression of SA-β-galactosidase. Conclusion: As mentioned above, we found that amentoflavone had effects on preventing skin aging and thought that amentoflavone can be a valulable material for antiaging cosmetics.

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