자외선 B에 의해 손상된 인간 각질형성세포(HaCaT)에 대한 Syringic Acid의 염증 억제 및 콜라겐 생성 효과
Anti-inflammatory and Collagen Production Effect of Syringic Acid on Human Keratinocyte (HaCaT) Damaged by Ultraviolet B
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목적: 본 연구는 자외선 B로 인해 손상된 인간 각질형성세포(HaCaT)에 대한 syringic acid의 염증 억제 및 콜라겐 생성 효과를 확인하여 기능성 화장품 소재로서 활용 가능성을 알아보기 위함이다. 방법: Syringic acid의 염증 억제 및 콜라겐 생성과 관련된 유전자 발현은 quantitative real-time polymerase chain reaction (qRT-PCR)을 통해 확인하였고, ELISA 분석으로 type I procollagen 의 생성량 변화를 확인하였다. 결과: Syringic acid는 자외선 B로 인해 손상된 인간 각질형성세포(HaCaT)에서 염증과 관련된 유전 자인 interleukin 6 (IL6), interleukin 1 beta (IL1β), cyclooxygenase 2 (COX2) 및 tumor necrosis factor alpha (TNFα)의 발현을 농도의존적으로 감소시켰다. 또한 콜라겐 전구체인 procollagen type I의 유전자 발현을 농도의존적으로 증가시켰으며 결과적으로 procollagen type I의 생산량도 농도의존적으로 증가시켰다. 결론: 본 연구를 통하여 syringic acid는 농도의존적으로 자외선 B에 의해 손상된 인간 각질형성세포의 염증을 억제하고, 피부 내 콜라겐 생성을 증가시킬 수 있는 천연유래 phytochemical 화장품 원료로서 활용가능하리라 사료된다. 다만 실제 화장품 원료로 피부에 적용하기 위해서는 추가적인 인체 대상 실험이 필요할 것으로 보인다.
Abstract
Purpose: The objective of this study is to examine the applicability of syringic acid as a cosmeceutical ingredient by examining its effects of inflammation suppression and collagen production for HaCaT (Cultured Human Keratinocyte) damaged by ultraviolet B. Methods: The gene expressions of syringic acid related to inflammation suppression and collagen production were confirmed through the quantitative realtime polymerase chain reaction (qRT-PCR). Changes in type I procollagen production were confirmed through the ELISA analysis. Results: Syringic acid reduced the expressions of the inflammation related genes-interleukin 6 (IL6), interleukin 1 beta (IL1β), cyclooxygenase 2 (COX2) and tumor necrosis factor alpha (TNFα)-in HaCaT damaged by ultraviolet B in a concentration dependent manner. It also increased the gene expression of the collagen precursor, procollagen type I, in the same manner, consequently increasing the production of procollagen type I in a concentration dependent manner. Conclusion: This study demonstrated the applicability of syringic acid as a natural phytochemical cosmeceutical ingredient that can suppress inflammation and produce collagen for HaCaT damaged by ultraviolet B in a concentration dependent manner. However, further human tests will be required to apply it to human skin as an actual cosmeceutical ingredient.
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