Smilax china 에탄올 추출물의 항염 및 피부 장벽 기능에 대한 효과
Effects of Smilax china Ethanol Extract on Anti-inflammation and Skin Barrier Function
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목적: 본 연구는 Smilax china 에탄올 추출물(SCR)이 항염증 및 피부 장벽 기능 개선에 미치는 영향을 조사하였으며, SCR이 염증성 피부질환을 완화할 수 있는 천연 소재로서의 가능성을 확인하였다. 방법: RAW 264.7 cells과 HaCaT cells의 세포 생존율은 MTT 분석을 통해 측정하였다. 항염증 활성은 NO 생성 억제, iNOS와 COX-2의 단백질 발현, 그리고 사이토카인의 mRNA 발현 수준을 확인하여 검증하였다. Type Ⅰ collagen 합성은 ELISA를 통해 확인하였으며, MMP-1 단백질 발현은 Western blot으로 측정하였고, 피부 장벽 기능 관련 mRNA 발현은 RT-PCR을 이용하여 측정하였다. 결과: SCR은 농도 의존적으로 NO 생성 억제, 전염증성 사이토카인(TNF-α, IL-1β, IL-6), 그리고 iNOS와 COX-2의 발현을 현저히 감소시켰다. 또한, SCR은 collagenase를 억제하고 HaCaT cells에서 Type Ⅰ collagen 합성을 촉진하였다. SCR은 MMP-1 생성을 감소시키고 필라그린(filaggrin), 인볼루크린(involucrin), 로리크린(loricrin) 등 피부 장벽 기능에 중요한 mRNA 발현을 증가시켰다. 결론: 이러한 결과는 SCR이 피부 장벽 기능 강화 및 항염 효과를 통해 염증성 피부질환의 증상 완화에 기여할 수 있음을 시사한다. 따라서 SCR은 화장품 산업에서 아토피 피부염 완화에 도움이 되는 기능성 소재로 활용 가능성이 높다고 판단된다.
Abstract
Purpose: This study investigated the effect of Smilax china ethanol extract (SCR) on improving anti-inflammatory and skin barrier function to confirm the potential of SCR as a natural material that can alleviate inflammatory skin diseases. Methods: The viability of macrophages (RAW 264.7 cells) and keratinocytes (HaCaT cells) following SCR treatment was assessed using the MTT assay. Anti-inflammatory activity was verified by confirming the inhibition of nitric oxide (NO) production, the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins, and the expression of cytokine mRNA. Collagen synthesis was confirmed by enzyme immunoassay. The expression of matrix metalloproteinase-1 (MMP-1) protein was determined using Western blot. The effect of SCR on skin barrier function was assessed using reverse-transcription polymerase chain reaction (RT-PCR) assay targeting filaggrin, involucrin, and loricrin mRNA. Results: SCR significantly reduced NO production, the levels of proinflammatory cytokines (tumor necrosis factor-α, interleukin [IL]-1β, and IL-6), and iNOS and COX-2 expression in a concentration-dependent manner. SCR also inhibited collagenase and promoted type Ⅰ collagen synthesis in HaCaT cells. Furthermore, SCR decreased MMP-1 production and enhanced the mRNA expression levels of filaggrin, involucrin, and loricrin, which are essential for skin barrier function. Conclusion: Our findings suggest that SCR contributes to the symptomatic relief of inflammatory skin diseases by enhancing skin barrier function and exerting an anti-inflammatory effect. Therefore, SCR may be a potential functional material that can be used by the cosmetic industry to help alleviate atopic dermatitis.
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