Prunin의 자외선 B에 의해 손상된 인간 각질형성세포 (HaCaT)에 대한 광노화 억제 효과
Inhibitory Effects of Prunin on Photo-aging in Human Keratinocytes (HaCaT) Damaged by UVB Radiation
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목적: 본 연구는 UVB로 인해 손상된 인간 각질형성세포(HaCaT)에 대한 prunin의 광노화에 효과가 있는지를 확인하여 노화에 효과적인 화장품 소재로서 활용 가능성을 알아보기 위함이다. 방법: Prunin의 UVB에 의한 증가된 MMP1 mRNA 발현과 UVB에 의해 감소된 COL1A1 mRNA의 발현은 quantitative real-time polymerase chain reaction (qRT-PCR)을 통해 확인하였고, luciferase reporter assay를 통해 MMP1 mRNA의 변화가 AP-1 전사 활성과 관련이 되어 있는지 분석하였다. 또한 ELISA 분석으로 type I procollagen의 생성량 변화를 확인하였다. 결과: Prunin은 UVB에 의한 세포독성으로부터 세포를 보호하고, UVB로 손상된 인간 각질형성세포(HaCaT)에서 산화 스트레스에 의해 증가된 MMP1 mRNA의 발현이 농도의존적으로 감소하였다. AP-1 전사활성 또한 농도의존적으로 감소하였다. Type I procollagen과 COL1A1 mRNA 발현이 농도의존적 증가하였다. 결론: 본 연구를 통하여 prunin이 산화 스트레스의 대표가 되는 자외선에 인한 노화 억제 효능이 확인되었으며 이는 광노화에 효과적인 화장품 원료로서 활용이 가능할 것으로 판단된다. 하지만 현실적으로 화장품 원료로 피부에 사용하기 위해서는 추후 인체를 대상으로 한 실험이 필요할 것이라 사료된다.
Abstract
Purpose: The aim of this study was to investigate the effects of prunin on photoaging in human keratinocytes (HaCaT) damaged by UVB radiation, so as to find out whether prunin can be used as an effective anti-aging ingredient in cosmetic products. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to identify the increased expression of MMP1 mRNA and decreased expression of COL1A1 mRNA due to UVB, and luciferase reporter assay was used to determine whether the change in MMP1 mRNA is related to AP-1 transcriptional activity. Furthermore, ELISA analysis was used to determine the change in type I procollagen production. Results: Prunin protected cells from UVB-induced cytotoxicity, and the increased expression of MMP1 mRNA due to oxidative stress in UVB-damaged HaCaT decreased in a concentration-dependent manner. AP-1 transcriptional activity also decreased in a concentration-dependent manner. The expression of Type I procollagen and COL1A1 mRNA increased in a concentration-dependent manner. Conclusion: This study found that prunin has an inhibitory efficacy on photoaging due to UV radiation, which is a leading cause of oxidative stress. Thus prunin can be used as an effective cosmetic raw material for inhibiting photoaging. However, it will be necessary to conduct experiments on the human body to find a practical way of applying prunin as a cosmetic raw material for the skin.
참고문헌 (23)
- Céliz G, "Antibacterial activity of naringin derivatives against pathogenic strains", Journal of Applied Microbiology, 111, pp.731~738 (2011)
- Céliz G, "Antimicrobial properties of prunin, a citric flavanone glucoside, and its prunin 6″-O-lauroyl ester", Journal of Applied Microbiology, 109, pp.1450~1457 (2010)
- Céliz G, "Prunin- and hesperetin glucoside-alkyl (C4-C18)esters interaction with Jurkat cells plasma membrane:consequences on membrane physical properties and antioxidant capacity", Food and Chemical Toxicology, 55, pp.411~423 (2013)
- Charous SJ, "Expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases in head and neck squamous cell carcinoma", The Annals of Otology, Rhinology & Laryngology, 106, pp.271~278 (1997)
- Choi JS, "Improvement of hyperglycemia and hyperlipemia in streptozotocin-diabetic rats by a methanolic extract of Prunus davidiana stems and its main component, pruning", Planta Medica, 57, pp.208~211 (1991)
- Chung JH, "Modulation of skin collagen metabolism in aged and photoaged human skin in vivo", The Journal of Investigative Dermatology, 117, pp.1218~1224 (2001)
- El-Domyati M, "Intrinsic aging vs. photoaging:comparative histopathological, immunohistochemical and ultrastructural study of skin", Experimental Dermatology, 11, pp.398~405 (2002)
- Fisher GJ, "Molecular basis of sun-induced premature skin ageing retinoid antagonism", Nature, 379, pp.335~339 (1996)
- Han XZ, "Protective effect of naringenin-7-O-glucoside against oxidative stress induced by doxorubicin in H9c2cardiomyocytes", Bioscience Trends, 6, pp.19~25 (2012)
- Han X, "Protective effects of naringenin-7-O-glucoside on doxorubicin-induced apoptosis in H9C2 cells", European Journal of Pharmacology, 581, pp.47~53 (2008)
- Itoh K, "Effects of unripe Citrus hassaku fruits extract and its flavanone glycosides on blood fluidity", Biological & Pharmaceutical Bulletin, 33, pp.659~664 (2010)
- Jung HA, "Inhibitory activity of flavonoids from Prunus davidiana and other flavonoids on total ROS and hydroxyl radical generation", Archives of Pharmacal Research, 26, pp.809~815 (2003)
- Kusukawa J, "The significance of epithelial growth factor receptor and matrix metalloproteinase-3 in squamous cell carcinoma of oral cavity", European Journal of Cancer Part B: Oral Oncology, 32B, pp.217~221 (1996)
- Lee YR, "Brazilin inhibits UVB-induced MMP-1", 3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts, 2012, pp.674, 80~86
- Mays PK, "Age-related changes in the proportion of type I and III collagen", Mechanisms of Ageing and Development, 45, pp.203~212 (1988)
- 나은주, "자외선 B에 의해 손상된 인간 각질형성세포(HaCaT)에 대한 Syringic Acid의 염증 억제 및 콜라겐 생성 효과", 아시안뷰티화장품학술지, 16(4), pp.523~531 (2018), 한국피부과학연구원[DOI]
- Perlish JS, "Identification of collagen fibrils in scleroderma skin", The Journal of Investigative Dermatology, 90, pp.48~54 (1998)
- Rittié L, "UV-light-induced signal cascades and skin aging", Ageing Research Reviews, 1, pp.705~720 (2002)
- Roshchin YV, "Anti-inflammatory activity of some flavonoids", Vopr Farm Dal’'nem Vostoke, 1, pp.135~ (1973)
- Saito Y, "Effects of a novel gaseous antioxidative system containing a rosemary extract on the oxidation induced by nitrogen dioxide and ultraviolet radiation", Bioscience , Biotechnology, and Biochemisty, 68, pp.781~786 (2004)
- Varani J, "Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis", Journal of Investigative Dermatology, 119, pp.122~129 (2002)
- Wlaschek M, "Solar UV irradiation and dermal photoaging", Journal of Photochemistry and Photobiology B, 63, pp.41~51 (2001)
- 윤영민, "UVB에 대한 Silibinin의 세포보호 기전에 작용하는 유전자 발현 프로파일링", 아시안뷰티화장품학술지, 11(1), pp.93~102 (2013), 한국피부과학연구원