Evaluation Biological Activities of Diphylleia grayi Extract as a Natural Cosmetic Ingredient
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Purpose: In this study, we investigated the biological activities of Diphylleia grayi extract as a natural cosmetic ingredient. Methods: The antioxidant activities of Diphylleia grayi extract was measured through 2,2-diphenyl-1-(2,4,6-trinitrophenyl)- hydrazinyl (DPPH), 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging assay and Fluorescence recovery after photobleaching (FRAP) assay. We further investigated whether Diphylleia grayi extract could reduce the intracellular reactive oxygen species (ROS) using dichlorofluorescin diacetate (DCF-DA) fluorescence. To investigate the whitening effect, we conducted tyrosinase inhibition assay and L-DOPA oxidation inhibition assay. Furthermore, we performed collagenase inhibition assay to evaluate the anti-wrinkle activity of Diphylleia grayi extract. Results: Diphylleia grayi extract showed strong antioxidant activities on DPPH, ABTS radical scavenging assay. Furthermore, Diphylleia grayi extract decreased the intracellular ROS on skin normal cells. Diphylleia grayi extract has also inhibition activities of tyrosinase, L-DOPA oxidation and collagenase. Conclusion: Therefore, Diphylleia grayi extract was identified as a promising natural cosmetic ingredient which has antioxidant, whitening and anti-wrinkle properties.
Abstract
Purpose: In this study, we investigated the biological activities of Diphylleia grayi extract as a natural cosmetic ingredient. Methods: The antioxidant activities of Diphylleia grayi extract was measured through 2,2-diphenyl-1-(2,4,6-trinitrophenyl)- hydrazinyl (DPPH), 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging assay and Fluorescence recovery after photobleaching (FRAP) assay. We further investigated whether Diphylleia grayi extract could reduce the intracellular reactive oxygen species (ROS) using dichlorofluorescin diacetate (DCF-DA) fluorescence. To investigate the whitening effect, we conducted tyrosinase inhibition assay and L-DOPA oxidation inhibition assay. Furthermore, we performed collagenase inhibition assay to evaluate the anti-wrinkle activity of Diphylleia grayi extract. Results: Diphylleia grayi extract showed strong antioxidant activities on DPPH, ABTS radical scavenging assay. Furthermore, Diphylleia grayi extract decreased the intracellular ROS on skin normal cells. Diphylleia grayi extract has also inhibition activities of tyrosinase, L-DOPA oxidation and collagenase. Conclusion: Therefore, Diphylleia grayi extract was identified as a promising natural cosmetic ingredient which has antioxidant, whitening and anti-wrinkle properties.
참고문헌 (21)
- Aguirre-Cruz G, "Collagen hydrolysates for skin protection : oral administration and topical formulation", Antioxidants, 9, pp.181~ (2020)
- Brenner M, "The protective role of melanin against UV damage in human skin", Photochemistry and Photobiology, 84, pp.539~549 (2007)
- Broomhead AJ, "Tumour-inhibitory aryltetralin lignans in Podophyllum versipelle, Diphylleia cymosa and Diphylleia grayi", Phytochemistry, 29, pp.3831~3837 (1990)
- Denat L, "Melanocytes as instigators and victims of oxidative stress", Journal of Investigative Dermatology, 134, pp.1512~1518 (2014)
- Farage MA, "Intrinsic and extrinsic factors in skin ageing : a review", International Journal of Cosmetic Science, 30, pp.87~95 (2008)
- Jiang N, "Azocoll protease activity assay", Protocol Exchange (2007)
- 심중현, "B16F10 세포에서 브라질린의 미백 효능", 아시안뷰티화장품학술지, 17(3), pp.287~294 (2019), 한국피부과학연구원[DOI]
- Krutmann J, "The skin aging exposome", Journal of Dermatological Science, 85, pp.152~161 (2017)
- 김경숙, "두피개선 화장품의 천연소재 개발 동향 - 식물추출물과 해양생물을 중심으로 -", 대한미용학회지, 16(2), pp.139~147 (2020), 대한미용학회[DOI]
- 김혜빈, "인간 진피섬유아세포에서 Chlorella sp. HS1 유래 오일의 콜라겐 발현 증가 효력에 관한 연구", 아시안뷰티화장품학술지, 18(1), pp.41~51 (2020), 한국피부과학연구원[DOI]
- 이기복, "Evaluation of Alcea rosea L. Callus Extract as a Natural Cosmetic Ingredient", 대한화장품학회지, 44(3), pp.295~302 (2018), 사단법인 대한화장품학회[DOI]
- Pittayapruek P, "Role of matrix metalloproteinases in photoaging and photocarcinogenesis", International Journal of Molecular Sciences, 17, pp.868~ (2016)
- Pillaiyar T, "Skin whitening agents : medicinal chemistry perspective of tyrosinase inhibitors", Journal of Enzyme Inhibition and Medicinal Chemistry, 32, pp.403~425 (2017)
- Poljšak B, "Free radicals and extrinsic skin aging", Dermatology Research and Practice, 2012, pp.135206~ (2012)
- Rinnerthaler M, "Oxidative stress in aging human skin", Biomolecules, 5, pp.545~589 (2015)
- Ribeiro A, "Main benefits and applicability of plant extracts in skin care products", Cosmetics, 2, pp.48~65 (2015)
- Rohani M, "Matrix remodeling by MMPs during wound repair", Matrix Biology, 44, pp.113~121 (2015)
- 류지영, "Prunin의 자외선 B에 의해 손상된 인간 각질형성세포 (HaCaT)에 대한 광노화 억제 효과", 아시안뷰티화장품학술지, 17(1), pp.139~147 (2019), 한국피부과학연구원[DOI]
- Samson N, "Interaction of skin color distribution and skin surface topography cues in the perception of female facial age and health", Journal of Cosmetic Dermatology, 10, pp.78~84 (2011)
- Yong J, "Bioinspired transparent underwater superoleophobic and anti-oil surfaces", Journal of Materials Chemistry A, 3, pp.9379~9384 (2015)
- Zhang S, "Fighting against skin aging", Cell Transplantation, 27, pp.729~738 (2018)