마늘추출물 함유 화장료의 피부 기능성 효과 : 항산화 및 콜라게나제, 엘라스타제와 티로시나제의 억제 효과를 중심으로
Effects of Formulation Containing Garlic Extract on Functional Skin Care: Antioxidation and Inhibitive Activities of Collagenase, Elastase and Tyrosinase
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We was to evaluate the physiological effects of formulation containing garlic extract on skin care. The total polyphenol (p<.001), flavonoid (p<.001) contents and 1,1-diphenyl–2-picrylhydrazyl (DPPH) (p<.001), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (p<.01) radicals scavenging activities and reducing power (p<.05) of the garlic formulation were significantly higher compared to those of the base formulation. The inhibition of collagenase (p<.05) of the garlic formulation were significantly higher compared to those of the base formulation. Specially, the base formulation inhibited slightly elastase activity whereas the garlic formulation had about 8-fold higher elastase inhibitory activity (p<.001). When the garlic extract was added to the formulation, the tyrosinase activity was decreased significantly (p<.05, p<.01, p<.001). In conclusion, the garlic formulation had high inhibitory activities for tyrosinase and elastase, thus suggesting that garlic may have beneficial properties as a material for cosmeceuticals.
Abstract
We was to evaluate the physiological effects of formulation containing garlic extract on skin care. The total polyphenol (p<.001), flavonoid (p<.001) contents and 1,1-diphenyl–2-picrylhydrazyl (DPPH) (p<.001), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (p<.01) radicals scavenging activities and reducing power (p<.05) of the garlic formulation were significantly higher compared to those of the base formulation. The inhibition of collagenase (p<.05) of the garlic formulation were significantly higher compared to those of the base formulation. Specially, the base formulation inhibited slightly elastase activity whereas the garlic formulation had about 8-fold higher elastase inhibitory activity (p<.001). When the garlic extract was added to the formulation, the tyrosinase activity was decreased significantly (p<.05, p<.01, p<.001). In conclusion, the garlic formulation had high inhibitory activities for tyrosinase and elastase, thus suggesting that garlic may have beneficial properties as a material for cosmeceuticals.
참고문헌 (49)
- Amagase H, "Impact of various sources of garlic and their constituents on 7,12-DMBA binding to mammary cell DNA", Carcinogenesis, 14, pp.1627~1631 (1993)
- Ankri S, "Antimicrobial properties of allicin from garlic", Microbes Infect, 2, pp.125~129 (1999)
- Arora DS, "Antimicrobial activity ofspices", Int. J. Antimicrob. Agents, 12, pp.257~262 (1999)
- Augusti KT, "Lipid lowering effect of allicin (diallyl disulfide oxide) on long-term feeding in normal rats", Experientia, 30, pp.468~470 (1974)
- Baciocchi E, "A kinetic study of the reaction of N, N-dimethylanilines with 2,2-diphenyl-1-picrylhydrazyl radical: a concerted proton-electron transfer?", J. Org. Chem, 73, pp.4110~4115 (2008)
- Banerjee SK, "Garlic as an antioxidant: the good, the bad and the ugly", Phytother. Res, 17, pp.97~106 (2003)
- Block E., "The chemistry of garlic and onion", Sci. Am, 252, pp.114~119 (1985)
- Chang LW, "Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark", Food Chem. Toxicol, 49, pp.785~790 (2011)
- Chen H, "Protection of vitamin-E, selenium, trolox-C, ascorbic-acid palmitate, acetylcysteine, coenzyme Q (0), beta-Carotene, canthaxanthin, and (+)-catechin against oxidative damage to rat-blood and tissues in-vivo", Free Radic. Biol. Med, 18, pp.949~953 (1995)
- Darvin ME, "Resonance Raman spectroscopy as an effective tool for the determination of antioxidative stability of cosmetic formulations", J. Biophotonics, 3, pp.82~88 (2010)
- Demina NS, "Collagenolytic enzymes synthesized by microorganisms", Mikrobiologiia, 65, pp.293~304 (1996)
- Dicko MH, "Evaluation of the effect of germination on phenolic compounds and antioxidant activities in sorghum varieties", J. Agric. Food Chem, 53, pp.2581~2588 (2005)
- Dorman HJD, "Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars", J. Agri. Food Chem, 51, pp.4563~4569 (2003)
- Ghalambor A, "Clinical study on the efficacy of orally administered crushed fresh garlic in controlling Pseudomonas aeruginosa infection in burn patients with varying burn degrees", Jundishapur J. Microbiol, 2, pp.7~13 (2009)
- Hajheydari Z, "Combination of topical garlic gel and betamethasone valerate cream in the treatment of localized alopecia areata: a double-blind randomized controlled study", Indian J. Dermatol. Venereol. Leprol, 73, pp.29~32 (2007)
- 홍양희, "자외선 B가 조사된 무모쥐 피부조직에 도포한 마늘화장품의 광보호 효과", 아시안뷰티화장품학술지, 10(3), pp.469~476 (2012), 주식회사 한국피부임상과학연구소
- James AEK, "Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor", Biochemistry, 35, pp.9090~9096 (1996)
- Kaur G, "Inhibition of oxidative stress and cytokine activity by curcumin in amelioration of endotoxin-induced experimental hepatoxicity in rodents", Clin. Exp. Immunol, 145, pp.313~321 (2006)
- Kim SR, "Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-κB. Signaling", PLoS ONE, 8, pp.1~12 (2013)
- Kim YJ, "4,4’-Dihydroxybiphenyl as a new potent tyrosinase inhibitor", Biol, Pharm, Bull, 28, pp.323~327 (2005)
- Kim YJ, "Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future", Cell. Mol. Life Sci, 62, pp.1707~1723 (2005)
- Lademann J, "Interaction between carotenoids and free radicals in human skin", Skin Pharmacol. Physiol, 24, pp.238~244 (2011)
- Ledezma E., "Efficacy of ajoene, an organosulphur derived from garlic, in the shortterm therapy of tinea pedis", Mycoses, 39, pp.393~395 (1996)
- Li CH, "Flavonoid composition and antioxidant activity of tree peony (Paeonia Section Moutan) yellow flowers", J. Agric. Food. Chem, 57, pp.8496~8503 (2009)
- Li X, "Concordance between antioxidant activities in vitro and chemical components of Radix Astragali (Huangqi)", Nat. Prod. Res, 26, pp.1050~1053 (2012)
- Li X, "Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui)", Molecules, 14, pp.5349~5361 (2009)
- Mason HS, "Melanoproteins I. Reactions between enzyme-generated quinones and amino acids", Biochim. Biophys. Acta, 111, pp.134~146 (1965)
- Miao YP, "Synthesis and antioxidative activity of 2-substituted phenyl-5-(3’-indolyl)-oxazole derivatives", Yao. Xue. Xue. Bao, 39, pp.37~40 (2004)
- Milner JA, "Garlic: its anticarcinogenic and antimutagenic properties", Nutr. Rev, 54, pp.S82~S86 (1996)
- Moriguchi T, "Anti-agenig effext of aged garlic extract in the inbred brain atrophy mouse model", Clin. Experimental Pharmacol. Physiol, 24, pp.235~242 (2007)
- Neil A, "Garlic; its cardio protective properties", Curr. Opin. Lipidol, 5, pp.6~10 (1994)
- Nishino H, "Antitumor promoting activity of allixin, a stress compound produced by garlic", Cancer J, 3, pp.20~21 (1990)
- Park SH, "Terrein: a new melanogenesis inhibitor and its mechanism", Cell Mol. Life Sci, 61, pp.2878~2885 (2004)
- Perez-Bernal A, "Management of facial hyperpigmen tation", Am. J. Clin. Dermatol, 1, pp.261~268 (2000)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol. Med, 26, pp.1231~1237 (1999)
- Reeve VE, "A garlic extract protects from ultraviolet B (280–320 nm) radiation induced suppression of contact hypersensitivity", Photochem. Photobiol, 58, pp.813~817 (1993)
- Resch KL, "Garlic (Allium sativum)-a potent medicinal plant", Fortschr. Med, 20, pp.311~315 (1995)
- Ruan ZP, "Evaluation of the antioxidant activity of Syzygium cumini leaves", Molecules, 13, pp.2545~2556 (2008)
- Scharffetter-Kochanek K, "Photoaging of the skin from phenotype to mechanisms", Exp. Gerontol, 35, pp.307~316 (2000)
- Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", Methods Enzymol, 299, pp.152~178 (1999)
- Steinmetz KA, "Vegetables, fruit and colon cancer in the Iowa woman’s study", J. Epidemiol, 139, pp.1~5 (1994)
- Van Wart HE, "A continuous spectrophotometric assay for Clostridium histolyticum collagenase", Anal. Biochem, 113, pp.356~365 (1981)
- Wei Z, "Garlic inhibits free radical generation and augments antioxidant enzyme activity in vascular endothelial cells", Nutr. Res, 18, pp.61~70 (1998)
- Wiedow O, "An elastase-specific inhibitor of human skin", J. Biol. Chem, 265, pp.14791~14795 (1990)
- Wiltried M, "Elastic fibre arrangement in the skin of the pig", Arch, Derm, Res, 270, pp.397~401 (1994)
- Wrona M, "Cooperation of antioxidants in protection against photosensitized oxidation", Free Radic. Biol. Med, 35, pp.1319~1329 (2003)
- Yan ZY, "Elastolytic activity from Flavobacterium odoratum. Microbial screening and cultivation, enzyme production and purification", Process Biochem, 29, pp.427~436 (1994)
- Yen GC, "Antioxidant activity of various tea extract in relation to their antimutagenicity", J. Agric. Food Chem, 43, pp.27~32 (1995)
- Zhu W, "Theuse of botanical extracts as topical skinlightening agents for the improvement of skin pigmentation disorders", J. Invest. Dermatol, 13, pp.20~24 (2008)
