은나노입자의 방사성 동위원소 운반체 적용 유효성 검증 연구
Feasibility Study on Silver Nanoparticle Application to a Radioisotope Carrier
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, an Ag-polyaniline-silica (Ag-PANI-silica) nanoparticle was evaluated asa radioisotope carrier. An Ag-PANI-silica nanoparticle was incubated in the 125I solution for aduration of 24 hr to test its radioisotope absorptivity. During the incubation, radioactivity of thenanoparticle was measured at 3, 6, 12, and 24 hr. After a 24 hr incubation, 125I-Ag-PANI-silicananoparticle was incubated in a fresh saline for a duration of 48 hr to check its stability. Additionally,the 125I-Ag-PANI-silica nanoparticle was injected to the ICR mouse to investigate its in-vivodistribution characteristics. The 125I absorption yield of the Ag-PANI-silica nanoparticle was higherthan 95% after a 6 hr incubation period in the 125I solution. And 125I-Ag-PANI-silica was stablefor 48 hr at 80% yield at room temperature. The SPECT/CT image of a mouse that received 125IAg-PANI-silica complex showed that the 125I-Ag-PANI-silica complex was distributed in the lung,stomach and thyroid at 30 min post injection. From these results, the Ag-PANI-silica nanoparticlehas good radio-iodine carrying property and can be applicable for the purpose of diagnosis andtherapy.
Abstract
In this study, an Ag-polyaniline-silica (Ag-PANI-silica) nanoparticle was evaluated asa radioisotope carrier. An Ag-PANI-silica nanoparticle was incubated in the 125I solution for aduration of 24 hr to test its radioisotope absorptivity. During the incubation, radioactivity of thenanoparticle was measured at 3, 6, 12, and 24 hr. After a 24 hr incubation, 125I-Ag-PANI-silicananoparticle was incubated in a fresh saline for a duration of 48 hr to check its stability. Additionally,the 125I-Ag-PANI-silica nanoparticle was injected to the ICR mouse to investigate its in-vivodistribution characteristics. The 125I absorption yield of the Ag-PANI-silica nanoparticle was higherthan 95% after a 6 hr incubation period in the 125I solution. And 125I-Ag-PANI-silica was stablefor 48 hr at 80% yield at room temperature. The SPECT/CT image of a mouse that received 125IAg-PANI-silica complex showed that the 125I-Ag-PANI-silica complex was distributed in the lung,stomach and thyroid at 30 min post injection. From these results, the Ag-PANI-silica nanoparticlehas good radio-iodine carrying property and can be applicable for the purpose of diagnosis andtherapy.
참고문헌 (18)
- 김수남, "은나노 입자의 크기 및 형태에 따른체내 동태 및 염증 반응", 환경보건독성학회지, 25(3), pp.215~222 (2010)
- Allen TM, "Drug deliverty systems entering the mainstream", Science, 303, pp.1818~1822 (2004)
- Badjic JD, "A molecular elevator", Science, 303, pp.1845~1849 (2004)
- Barth JV, "Engineering atomic and molecular nanostructure at surface", Nature, 437, pp.671~679 (2005)
- Bruchez M, "Semi-conductor nanocrystals as fluorescent biological labels", Science, 281, pp.2013~2016 (1998)
- Cui Y, "Functional nanoscale electornic devices assembled using silicon nanowire building blocks", Science, 291, pp.851~853 (2001)
- Jin R, "Photoinduced conversion of silver nanoprisms", Science, 294, pp.1901~1903 (2001)
- 강은아, "분자영상을 위한 다기능성 나노입자", 대한의사협회지, 52(2), pp.125~134 (2009), 대한의사협회
- Kaul G, "Long-circulating poly(ethylene glycol)-modified gelatin nanoparticles for intracellular delivery", Pharm. Res, 19, pp.1061~1067 (2002)
- Kim HJ, "Synthesis of a new electrically conducting nanosized Ag-polyaniline-silica complex using γ-radiolysis and its biosensing application", Radiat. Physics and Chem, 79, pp.894~899 (2010)
- Maeda H, "Tumor vascular permeability and EPR effect in macromolecular therapeutics : a review", J. Control Release, 65, pp.271~284 (2000)
- Milliron DJ, "Colloidal nanocrystal heterostructures with linear and branched topology", Nature, 430, pp.190~195 (2004)
- Mirkin CA, "A DNA-based method for rationally assembling nanoparticles into macroscopic materials", Nature, 382, pp.607~609 (1996)
- Ouyang M, "Energy gaps in metallic single-walled carbon nanotubes", Science, 292, pp.702~705 (2001)
- Rosi NL, "Oligonuclide-modified gold nanoparticles for intracellular gene regulation", Science, 312, pp.1027~1030 (2006)
- Sanvinces N, "Multifuctional nanoparticles-properties and prospects for their use in human medicine", Trends Biotechnol, 26, pp.425~433 (2008)
- Tochilin VP, "Multifunctional nanocarriers", Adv. Drug. Deliv. Rev, 58, pp.1532~1555 (2006)
- Yin Y, "Colloidal nanocrystal synthesis and the organic-inorganic interface", Nature, 437, pp.664~670 (2005)