양성자 및 감마선처리에 의한 유채 내염성 변이계통 선발
Selection of Salt Tolerant Rapeseed (Brassica napus L.) Mutant Lines Induced by Irradiation of Proton Ion Beams and Gamma Ray
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This study was carried out to develop salt tolerant varities of rapeseed (Brassica napusL.) which can be grown in the high salty reclaimed land. The seeds of three varieties ‘Naehan’,‘Tammi’, and ‘Halla’ were treated by proton ion beams and gamma rays with 0 to 2,000 Gy. Forthe selection of salt tolerant lines, emergence and survival rate, and growth characteristics of M2to M4 generations were investigated in the Saemangeum reclaimed fields with the different saltconcentrations. The lines with potential salt tolerance were selected in the M4 generation andtested indoor for their growth characteristics. There was no significant changes in the soil pH forM4 generation during growth period. However, soil EC was higher in early spring than sowingperiod (mid October). In M4 generation test, the seeds of original and selected line showed highrates of emergence and survival, as determined one month after sowing. After wintering, however,the original varieties showed the significant reduction in the survival rate, while the selectedlines showed a higher survival rate and good growth, leading to the completion of their life cycle. Consequently we selected 9 lines from M4 generation with better performance in growth andyield. Soil EC was 2.8~4.3 dS∙m-1 during M4 generation growth period. The laboratory test ofthe lines selected from M4 generation was made for their salt tolerance potential. The selectedlines showed higher chlorophyll and proline contents than the original varieties. There was alsono significant difference in the emergence rate of seed between the original and selected varieties. In 200 mM natural sea salt, the Nγ600-21-1-641 line derived from ‘Naehan’ was the highest ingrowth rate, leaf chlorophyll and proline contents. Tγ800-20-2-461 line derived from ‘Tammi’didn’t show significant difference in growth rate compared to original variety in 200 mM andwithered in 250 mM like other lines as time passed. Hγ200-7-1-740 line showed similar growthand chlorophyll content compared to its original variety.
Abstract
This study was carried out to develop salt tolerant varities of rapeseed (Brassica napusL.) which can be grown in the high salty reclaimed land. The seeds of three varieties ‘Naehan’,‘Tammi’, and ‘Halla’ were treated by proton ion beams and gamma rays with 0 to 2,000 Gy. Forthe selection of salt tolerant lines, emergence and survival rate, and growth characteristics of M2to M4 generations were investigated in the Saemangeum reclaimed fields with the different saltconcentrations. The lines with potential salt tolerance were selected in the M4 generation andtested indoor for their growth characteristics. There was no significant changes in the soil pH forM4 generation during growth period. However, soil EC was higher in early spring than sowingperiod (mid October). In M4 generation test, the seeds of original and selected line showed highrates of emergence and survival, as determined one month after sowing. After wintering, however,the original varieties showed the significant reduction in the survival rate, while the selectedlines showed a higher survival rate and good growth, leading to the completion of their life cycle. Consequently we selected 9 lines from M4 generation with better performance in growth andyield. Soil EC was 2.8~4.3 dS∙m-1 during M4 generation growth period. The laboratory test ofthe lines selected from M4 generation was made for their salt tolerance potential. The selectedlines showed higher chlorophyll and proline contents than the original varieties. There was alsono significant difference in the emergence rate of seed between the original and selected varieties. In 200 mM natural sea salt, the Nγ600-21-1-641 line derived from ‘Naehan’ was the highest ingrowth rate, leaf chlorophyll and proline contents. Tγ800-20-2-461 line derived from ‘Tammi’didn’t show significant difference in growth rate compared to original variety in 200 mM andwithered in 250 mM like other lines as time passed. Hγ200-7-1-740 line showed similar growthand chlorophyll content compared to its original variety.
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