감마선 및 EMS처리에 의한 유채(Brassica napus L.)와갓(Brassica juncea L.)의 유묘 감수성 평가 및형태적 변이체 선발
Determination of Seedling Sensitivity and Selection of Morphological Mutants by Treatments of Gamma-Ray and EMS in Rapeseed and Leaf Mustard
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초록
This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leafmustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated withgamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration toenlarge the characteristic morphological variations were also separately investigated. The survivalrates of S-28 only linearly decreased with increasing the gamma-ray dose. The overallgrowth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. Thereduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those ofS-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 andS-27 showed no significant differences by EMS treatment, while the growth of all three lines weresignificantly decreased. The reduction concentration 50 in S-14 could not be determined,demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, whilethose in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseedlines possess higher sensitivity to EMS than S-14. Various morphological characteristics of M1generation obtained from mutagen treatment were elaborately investigated for further maintenanceof M2 generation. In M2 generation variants showing short stem, yellow color in seed coat,chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially usefulmutants for breeding.
Abstract
This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leafmustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated withgamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration toenlarge the characteristic morphological variations were also separately investigated. The survivalrates of S-28 only linearly decreased with increasing the gamma-ray dose. The overallgrowth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. Thereduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those ofS-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 andS-27 showed no significant differences by EMS treatment, while the growth of all three lines weresignificantly decreased. The reduction concentration 50 in S-14 could not be determined,demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, whilethose in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseedlines possess higher sensitivity to EMS than S-14. Various morphological characteristics of M1generation obtained from mutagen treatment were elaborately investigated for further maintenanceof M2 generation. In M2 generation variants showing short stem, yellow color in seed coat,chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially usefulmutants for breeding.
참고문헌 (19)
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