방사선 유도 내염성 증진 사료용 옥수수 돌연변이체 특성 분석
Characterization of Gamma-radiation Induced Salt-tolerant Silage Maize Mutant
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초록
Salt stress is a significant factor limiting growth and productivity of crops. However, little is known about the response and resistance mechanism to salt stress in maize. The objective of this research is to develop an enhanced salt-tolerant silage maize by mutagenesis with gamma radiation. To generate gamma radiation-induced salt-tolerant silage maize, we irradiated 100 gray (Gy) gamma rays to KS140 inbred line. Salt tolerance was evaluated by the plant growth, morphological changes, and gene expression under NaCl stress. We screened 10 salt-tolerant maize inbred lines from 2,248 M2 mutant populations and selected a line showing better growth under salt stress conditions. The selected 140RS516 mutant exhibited improved seed germination and plant growth compared with wild-type under salt stress conditions. Enhanced salt tolerance of the 140RS516 mutant was attributed to higher stomatal conductance and proline contents. In whole-genome re-sequencing analysis, a total of 328 single nucleotide polymorphisms (SNPs) and insertions or deletions (InDels) were identified in the 140RS516 mutant. We found that the expression of the genes involved in salt stress tolerance, ABP9, CIPK21, and CIPK31, were increased by salt stress in the 140RS516 mutant. Our results suggest that the 140RS516 mutant induced by gamma rays could be a good material for developing cultivars with salt tolerance in maize.
Abstract
Salt stress is a significant factor limiting growth and productivity of crops. However, little is known about the response and resistance mechanism to salt stress in maize. The objective of this research is to develop an enhanced salt-tolerant silage maize by mutagenesis with gamma radiation. To generate gamma radiation-induced salt-tolerant silage maize, we irradiated 100 gray (Gy) gamma rays to KS140 inbred line. Salt tolerance was evaluated by the plant growth, morphological changes, and gene expression under NaCl stress. We screened 10 salt-tolerant maize inbred lines from 2,248 M2 mutant populations and selected a line showing better growth under salt stress conditions. The selected 140RS516 mutant exhibited improved seed germination and plant growth compared with wild-type under salt stress conditions. Enhanced salt tolerance of the 140RS516 mutant was attributed to higher stomatal conductance and proline contents. In whole-genome re-sequencing analysis, a total of 328 single nucleotide polymorphisms (SNPs) and insertions or deletions (InDels) were identified in the 140RS516 mutant. We found that the expression of the genes involved in salt stress tolerance, ABP9, CIPK21, and CIPK31, were increased by salt stress in the 140RS516 mutant. Our results suggest that the 140RS516 mutant induced by gamma rays could be a good material for developing cultivars with salt tolerance in maize.
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