감마선과 전자선 조사처리된 들깨와 대두의 신속 판별을 위한 유전자 코메트 분석
Comet Assay for Rapid Identification of Irradiated Perilla Seeds and Soybeans with Gamma Rays and Electron-beams
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초록
This study was carried out to investigate the applicability of comet assay, which isapproved as a formal detection method only for the identification of irradiated fresh meats inKorea, to identify irradiated perilla seeds and soybeans at doses at 0, 0.5, 1, 2, and 3 kGy withgamma rays or electron-beams (e-beams). The florescent images for comet assay were obtained bymicroscopic observation and olive tail moment (OTM) of nuclei in a samples was measured by usinga comet analysis software. Irradiation with both gamma rays and e-beams increased the ratio ofconsiderably damaged nuclei (OTM≥30) in both perilla seeds and soybeans as the absorbed doseincreased. Moreover, the average OTM exhibited close correlations with the absorbed dose in bothfood models, regardless of radiation sources (R2 of regression curves: 0.91~0.98). On the otherhand, all samples irradiated at more than 1 kGy could be discriminated from non-irradiated oneby visual inspection of typical comets of nuclei generated by irradiation using fluorescent microscopewithout the comet analysis software. Based on these results, it is considered that the cometassay can be used as a rapid identification method for irradiated perilla seeds and soybeans withgamma rays and e-beams, because the evaluation for irradiation using this method can be drawnwithin 3 hours.
Abstract
This study was carried out to investigate the applicability of comet assay, which isapproved as a formal detection method only for the identification of irradiated fresh meats inKorea, to identify irradiated perilla seeds and soybeans at doses at 0, 0.5, 1, 2, and 3 kGy withgamma rays or electron-beams (e-beams). The florescent images for comet assay were obtained bymicroscopic observation and olive tail moment (OTM) of nuclei in a samples was measured by usinga comet analysis software. Irradiation with both gamma rays and e-beams increased the ratio ofconsiderably damaged nuclei (OTM≥30) in both perilla seeds and soybeans as the absorbed doseincreased. Moreover, the average OTM exhibited close correlations with the absorbed dose in bothfood models, regardless of radiation sources (R2 of regression curves: 0.91~0.98). On the otherhand, all samples irradiated at more than 1 kGy could be discriminated from non-irradiated oneby visual inspection of typical comets of nuclei generated by irradiation using fluorescent microscopewithout the comet analysis software. Based on these results, it is considered that the cometassay can be used as a rapid identification method for irradiated perilla seeds and soybeans withgamma rays and e-beams, because the evaluation for irradiation using this method can be drawnwithin 3 hours.
참고문헌 (38)
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