청하향초(Tagetes minuta) 꽃과 줄기 추출물의 미백, 항주름 효과 및 항산화 활성
Effects of Whitening and Anti-wrinkling and Antioxidant Activities of Flower and Stem Extracts of Tagetes minuta
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국화과(Compositae)에 속하는 청하향초(Tagetes minuta)는 간질환, 장내 질환, 설사, 복통, 두통, 폐렴 및 곰팡이성 피부질환 등의 치료에 응용하는 생약자원이다. 본 연구는 한방에서 한방생약재로 사용되는 청하향초를 새로운 기능성소재로 개발하기 위한 연구의 일환으로 청하향초의 꽃과 줄기를 물과 에탄올로 추출하여 항산화효과, 미백 및 주름개선 효과를 분석하였다. 청하향초의 폴리페놀 및 플라보노이드 화합물을 측정한 결과, 꽃의 에탄올 추출물인 FRE에서 각각 426.42 mg/g과 61.11 mg/g을 함유하였으며, SOD 유사활성능은 1.0 mg/mL의 농도에서 줄기 물 추출물인 SRW가 62.53%로 가장 우수한 활성을 나타내었고, 꽃 물 추출물인 FRW에서도 61.92%의 SOD 유사활성을 나타내었다. 상이한 용매로 추출한 청하향초 꽃과 줄기의 추출물을 농도(0.1~1.0 mg/mL)에 따라 전자공여능을 측정한 결과, 청하향초 꽃의 FRW 84~93.26%, FRE 75.08~89.94%로 분석되었으며, 줄기의 SRW 55.72~92.64%, SRE 64.65~92.91%의 활성을 나타내었다. 아질산염 소거능은 pH 1.2의 1.0 mg/mL의 농도에서 FRE 추출물이 92.28%로 84.32%의 활성을나타낸 ascorbic acid보다 높은 소거활성을 나타내었으며 줄기는 물 추출물인 SRW에서 38.80%의 효과를 나타내었다. 청하향초 꽃과 줄기 추출물의 tyrosinase 저해활성은 줄기의물 추출물인 SRW에서 25.45%로 가장 높은 활성을 나타내었고, collagenase 저해능은 1.0 mg/mL농도에서 꽃과 줄기에탄올 추출물에서 각각 69%와 45%의 활성을 나타내었다. 피부의 주름개선에 효과가 있는 elastase 저해능은 1.0 mg/mL의 농도에서 청하향초 줄기의 에탄올 추출물인 SRE에서 46.73%로 가장 높은 효과를 나타내었다. 이상의 결과,민간에서 약용으로 이용되고 있는 청하향초의 꽃과 줄기는우수한 항산화 활성과 미백 및 주름개선효과를 지닌 천연소재임을 확인하였으며, 이를 이용한 미용제품 및 기능성제품의 원료 및 첨가재로 활용할 수 있을 것이라 사료된다.
Abstract
Tagetes minuta L., belonging to the Chrysanthemum family has anti-inflammatory, antibacterial or antimicrobial effect. It has been used as not only an ornamental plant but also for flavoring, coloring or as medical plants in foreign countriessince a long time ago. This study was carried out to investigate effects of whitening and anti-wrinkling and antioxidantactivities of flower and stem extracts of Tagetes minuta with water and ethanol. In the contents of total polyphenol andflavonoid compounds, ethanolic extract of flower (FRE) was the highest as 426.42 mg/g and 61.11 mg/g, respectively. Inthe electron donating ability, the water extract of flower (FRW) and water extract of stem (SRW) were 93.26% and 92.91%,respectively, at the concentration of 1.0 mg/mL. In the superoxide dismutase (SOD)-like activity, ethanolic extract ofstem (SRE) was the highest as 62.53% and water extract of flower (FRW) was 61.92% at 1.0 mg/mL. In the nitrate scavengingability, FRW was the highest as 93.18% and the SRW was 92.27% at pH 1.2 and 1.0 mg/mL. The tyrosinase inhibitionactivity of SRW was 25.45% and the collagenase inhibition activity of SRE was 45% at 1.0 mg/mL. The elastaseinhibition activity of SRE was 46.73% at 1.0 mg/mL. These results suggest that the extract of T. minuta flower and stemcould be used as anti-wrinkling agent and antioxidant resources for functional foodstuff, cosmetics and beauty industrialmaterials.
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