Melandrium firmum Extract Promotes Hair Growth by Modulating 5α-Reductase Activity and Gene Expression in C57BL/6J Mice
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Background: In our preliminary study, we screened for their potential to inhibit 5α-reductase, and Melandrium firmum (MF) extract showed the most potent activity as confirmed by high-performance liquid chromatography (HPLC). Objective: This study aimed to investigate the effects of MF extract on 5 α-reductase activity and its mechanisms of action in the prevention or treatment of androgenetic alopecia. Methods: HPLC was used to measure 5α-reductase activity. The hair growth-promoting effect of MF extract in the shaved dorsal skin of C57BL/6J mice was studied for 30 days. Hair follicles were examined by histological examination. Protein and mRNA levels of growth factors involved in hair growth were determined by western blotting, and reverse transcriptionpolymerase chain reaction (RT-PCR) and qPCR, respectively. Cell proliferation was measured by (3-(4, 5-dimethylthiazol- 2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium) assay. Results: MF extract at 0.5 mg/ml showed 43.5% inhibition of 5α-reductase. MF extract promoted hair growth by inducing anagen phase reflected by skin color, hair density, and the number and size of hair follicles. It not only reduces the expression of transforming growth factor-beta 1 (TGF-β1) and Dickkopf-1 (DKK-1), but also markedly upregulated insulin-like growth factor 1 and keratinocyte growth factor in the dorsal dermal tissue. Ursolic acid, ecdcysteron, and ergosterol peroxide were identified as active constituents by activity-guided fractionation to inhibit 5α-reductase. They decreased the gene expression of TGF-β1 and DKK-1 in human hair dermal papilla cells. Conclusion: In summary, these finding indicate that MF extract might be a good drug candidate for hair growth promotion. (
Abstract
Background: In our preliminary study, we screened for their potential to inhibit 5α-reductase, and Melandrium firmum (MF) extract showed the most potent activity as confirmed by high-performance liquid chromatography (HPLC). Objective: This study aimed to investigate the effects of MF extract on 5 α-reductase activity and its mechanisms of action in the prevention or treatment of androgenetic alopecia. Methods: HPLC was used to measure 5α-reductase activity. The hair growth-promoting effect of MF extract in the shaved dorsal skin of C57BL/6J mice was studied for 30 days. Hair follicles were examined by histological examination. Protein and mRNA levels of growth factors involved in hair growth were determined by western blotting, and reverse transcriptionpolymerase chain reaction (RT-PCR) and qPCR, respectively. Cell proliferation was measured by (3-(4, 5-dimethylthiazol- 2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium) assay. Results: MF extract at 0.5 mg/ml showed 43.5% inhibition of 5α-reductase. MF extract promoted hair growth by inducing anagen phase reflected by skin color, hair density, and the number and size of hair follicles. It not only reduces the expression of transforming growth factor-beta 1 (TGF-β1) and Dickkopf-1 (DKK-1), but also markedly upregulated insulin-like growth factor 1 and keratinocyte growth factor in the dorsal dermal tissue. Ursolic acid, ecdcysteron, and ergosterol peroxide were identified as active constituents by activity-guided fractionation to inhibit 5α-reductase. They decreased the gene expression of TGF-β1 and DKK-1 in human hair dermal papilla cells. Conclusion: In summary, these finding indicate that MF extract might be a good drug candidate for hair growth promotion. (
참고문헌 (26)
- Kerscher M, "Cosmetic dermatology and skin care", Eur J Dermatol, 17, pp.180~182 (2007)
- Paik JH, "The prevalence and types of androgenetic alopecia in Korean men and women", Br J Dermatol, 145, pp.95~99 (2001)
- 강정일, "Hair-Loss Preventing Effect of Grateloupia elliptica", Biomolecules & Therapeutics, 20(1), pp.118~124 (2012), 한국응용약물학회
- Ellis JA, "Androgenetic alopecia : pathogenesis and potential for therapy", Expert Rev Mol Med, 4, pp.1~11 (2002)
- Hadshiew IM, "Burden of hair loss : stress and the underestimated psychosocial impact of telogen effluvium and androgenetic alopecia", J Invest Dermatol, 123, pp.455~457 (2004)
- Burton JL, "Hypertrichosis due to minoxidil", Br J Dermatol, 101, pp.593~595 (1979)
- Kaufman KD, "Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group", J Am Acad Dermatol, 39, pp.578~589 (1998)
- Shorter K, "Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil", FASEB J, 22, pp.1725~1736 (2008)
- Murata K, "Promotion of hair growth by Rosmarinus officinalis leaf extract", Phytother Res, 27, pp.212~217 (2013)
- Patel S, "Hair growth : focus on herbal therapeutic agent", Curr Drug Discov Technol, 12, pp.21~42 (2015)
- Kang JI, "Promotion effect of Schisandra nigra on the growth of hair", Eur J Dermatol, 19, pp.119~125 (2009)
- Park HJ, "Topical application of Polygonum multiflorum extract induces hair growth of resting hair follicles through upregulating Shh and β-catenin expression in C57BL", 6 mice, 2011, pp.135, 369~375
- Md. Ataur Rahman, "Apoptotic Effects of Melandryum firmum Root Extracts in Human SH-SY5Y Neuroblastoma Cells", Experimental Neurobiology, 22(3), pp.208~213 (2013), 한국뇌신경과학회
- Ming Shan Zheng, "Chemical Constituents of Melandrium firmum Rohrbach and Their Anti-Inflammatory Activity", Archives of Pharmacal Research, 31(3), pp.318~322 (2008), 대한약학회
- Matsuda H, "Testosterone 5alpha-reductase inhibitory active constituents from Anemarrhenae Rhizoma", Biol Pharm Bull, 24, pp.586~587 (2001)
- Müller-Röver S, "A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages", J Invest Dermatol, 117, pp.3~15 (2001)
- Tan JJY, "Bioactives in Chinese proprietary medicine modulates 5α-reductase activity and gene expression associated with androgenetic alopecia", Front Pharmacol, 8, pp.194~ (2017)
- Inui S, "Androgen-inducible TGF-beta1 from balding dermal papilla cells inhibits epithelial cell growth : a clue to understand paradoxical effects of androgen on human hair growth", FASEB J, 16, pp.1967~1969 (2002)
- Inui S, "Identification of androgen-inducible TGF-beta1 derived from dermal papilla cells as a key mediator in androgenetic alopecia", J Investig Dermatol Symp Proc, 8, pp.69~71 (2003)
- Kwack MH, "Dihydrotestosterone-inducible dickkopf 1 from balding dermal papilla cells causes apoptosis in follicular keratinocytes", J Invest Dermatol, 128, pp.262~269 (2008)
- Andl T, "WNT signals are required for the initiation of hair follicle development", Dev Cell, 2, pp.643~653 (2002)
- Zhao J, "Dietary isoflavone increases insulin-like growth factor-I production, thereby promoting hair growth in mice", J Nutr Biochem, 22, pp.227~233 (2011)
- Rajendran RL, "Extracellular vesicles derived from MSCs activates dermal papilla cell in vitro and promotes hair follicle conversion from telogen to anagen in mice", Sci Rep, 7, pp.15560~ (2017)
- Werner S, "The function of KGF in morphogenesis of epithelium and reepithelialization of wounds", Science, 266, pp.819~822 (1994)
- Jena AK, "Amelioration of testosterone induced benign prostatic hyperplasia by Prunus species", J Ethnopharmacol, 190, pp.33~45 (2016)
- Christensen KB, "Identification of bioactive compounds from flowers of black elder (Sambucus nigra L.) that activate the human peroxisome proliferator-activated receptor (PPAR) gamma", Phytother Res, (24 Suppl 2), pp.S129~S132 (2010)