RAW 264.7 cell에서의 미모사 에탄올 추출물의 항산화 및 항염증 효능 연구
Antioxidant and Anti-Inflammatory Effects of Mimosa Pudica Ethanol Extract in RAW 264.7 Cell
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The purpose of this study is to identify the possibility of Mimosa pudica ethanol extracts (MEE) as a cosmeceutical material with antioxidant and anti-inflammatory effects. At first, the antioxidant effect of the MEE was carried out through Total polyphenol, 2,2diphenyl-1-picrylhydrazyl (DPPH) electro-donating activity, 2,2’-azino-bis diammonium salt (ABTS+) radial scavenging activity. The total polyphenol content of the MEE was 3.514 mg/g. DPPH electro-donating activity of MEE were increased in a dose-dependent manner (P<0.05), and was about 88.1% at 1 mg/mL concentration. Also, ABTS+ radical scavenging activity of MEE were remarkably increased in a dose-dependent manner (P<0.05), and was about 99.7% at 1 mg/mL concentration. The MEE showed relatively high antioxidant effects in most experiments. To evaluate the anti-inflammatory effects in RAW 264.7 cells, the maximum permissible concentration was verified first by cell viability assessment using MTT assay. And then, nitric oxide (NO) production inhibition and amounts of inflammatory related proteins and gene expressions were identified after LPS treatment. NO inhibition of MEE was decreased concentration dependently, and was about 84.3% at 1 mg/mL concentration. And TNF-α and IL-6 proteins by western blotting were also decreased significantly by treatment of MEE. As a result of measuring the expression levels of TNF-α and IL-6, the expression inhibition rates were 67.87% and 71.91%, respectively. Based on the results of these experiments, we suggest that MEE could be useful as a cosmeceutical material with anti-inflammatory effects.
Abstract
The purpose of this study is to identify the possibility of Mimosa pudica ethanol extracts (MEE) as a cosmeceutical material with antioxidant and anti-inflammatory effects. At first, the antioxidant effect of the MEE was carried out through Total polyphenol, 2,2diphenyl-1-picrylhydrazyl (DPPH) electro-donating activity, 2,2’-azino-bis diammonium salt (ABTS+) radial scavenging activity. The total polyphenol content of the MEE was 3.514 mg/g. DPPH electro-donating activity of MEE were increased in a dose-dependent manner (P<0.05), and was about 88.1% at 1 mg/mL concentration. Also, ABTS+ radical scavenging activity of MEE were remarkably increased in a dose-dependent manner (P<0.05), and was about 99.7% at 1 mg/mL concentration. The MEE showed relatively high antioxidant effects in most experiments. To evaluate the anti-inflammatory effects in RAW 264.7 cells, the maximum permissible concentration was verified first by cell viability assessment using MTT assay. And then, nitric oxide (NO) production inhibition and amounts of inflammatory related proteins and gene expressions were identified after LPS treatment. NO inhibition of MEE was decreased concentration dependently, and was about 84.3% at 1 mg/mL concentration. And TNF-α and IL-6 proteins by western blotting were also decreased significantly by treatment of MEE. As a result of measuring the expression levels of TNF-α and IL-6, the expression inhibition rates were 67.87% and 71.91%, respectively. Based on the results of these experiments, we suggest that MEE could be useful as a cosmeceutical material with anti-inflammatory effects.
참고문헌 (22)
- Balakrishnan N, "Antidiarrhoeal potential of Mimosa pudica root extracts", Indian Journal of Natural Products, 22(2), pp.21~23 (2006)
- Blois MS., "Antioxidant Determinations by the Use of a Stable Free Radical", Nature, 181, pp.1199~1200 (1958)
- Bum EN, "Anticonvulsant activity of Mimosa pudica decoction", Fitoterapia, 75(3-4), pp.309~314 (2004)
- Chowdhury SA, "Cytotoxicity, antimicrobial and antioxidant studies of the different plant parts of Mimosa pudica", Stamford journal of pharmaceutical sciences, 1(1), pp.80~84 (2008)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Ganguly M, "Effect of Mimosa pudica root extract on vaginal estrous and serum hormones for screening of antifertility activity in albino mice", Contraception, 76(6), pp.482~485 (2007)
- Ghani A, "Medicinal plants of Bangladesh: chemical constituents and uses", Asiatic society of Bangladesh, ~ (1998)
- Guha M, "LPS induction of gene expression in human monocytes", Cellular Signal, 13, pp.85~94 (2001)
- Higuchi M, "Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages", The Journal of Immunology, 144(4), pp.1425~1431 (1990)
- 김대용, "산딸기 잎 추출물의 항염증 기능 탐색에 관한 연구", 산업식품공학, 20(1), pp.21~27 (2016), 한국산업식품공학회
- Kubes P, "Nitric oxide and intestinal inflammation", American J Medicine, 109, pp.150~158 (2000)
- 곽호영, "누룩(Rhizopus oryzae KSD-815)으로부터 분리한 지질화합물의세포독성 및 항염증 활성", Applied Biological Chemistry, 51(2), pp.142~147 (2008), 한국응용생명화학회
- Lee YS, "IL-6 mRNA expression in mouse peritoneal macrophages and NIH3T3 fibroblasts in response to Candida albicans", Journal of microbiology and biotechnology, 10(1), pp.8~15 (2000)
- 이초은, "RAW 264.7 대식세포와 염증유도 동물모델에서 산겨릅나무 추출물의 항산화 및 항염증 효과", 한국식품영양과학회지, 46(1), pp.1~9 (2017), 한국식품영양과학회
- Lin WW, "cytokine-mediated link between innate immunity, inflammation, and cancer", J Clin Invest, 117, pp.1175~1183 (2007)
- Lowry OH, "Protein measurement with the Folin phenol reagent", Journal of biological chemistry, 193, pp.265~275 (1951)
- Mu MM, "The inhibitory action of quercetin on lipopolysaccharide induced nitric oxide production in RAW 264.7 macrophage cells", J Endotoxin Res, 7, pp.431~438 (2001)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Ryu JH, "Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages", Phytother Res, 17, pp.485~489 (2003)
- Sohil V, "Hepatoprotective activity of Mimosa pudica leaves against Carbontetrachloride induced toxicity", Journal of Natural Products, 2, pp.116~122 (2009)
- Umamaheswari S, "Antihyperglycaemic effect of ëilogen-excelí, an ayurvedic herbal formulation in streptozotocin-induced diabetes mellitus", Acta Poloniae Pharmaceutica-Drug Research, 64(1), pp.53~61 (2007)
- 우현심, "LPS로 유도된 RAW264.7 대식세포에 대한헛개나무(Hovenia dulcis) 추출물의 항염증 효과", 한국자원식물학회지, 31(5), pp.466~477 (2018), 한국자원식물학회[DOI]