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몰로키아(Corchorus olitirius L.) 추출물의 생리활성에 대한 연구

Study on the Physiological Activity of Corchorus olitirius L.

유단희|이인철
한국인체미용예술학회 (The Korean Society of Beauty and Art)|2021.06|한국인체미용예술학회지 (The Korean Society Of Beauty And Art)|제22권 제2호|pp. 275-289|DOI http://doi.org/10.18693/jksba.2021.22.2.275

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키워드

Antioxidant항염증항산화미백효과몰로키아Anti-inflammatoryWhitening EffectCorchorus olitirius L.
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초록

In this study, the effects of the physiological activity of the Corchorus olitirius L. were verified and the applicability as a cosmetic material was reviewed. The total polyphenol content was expressed as 396.44±0.42 mg/100g dry in the water extract of Corchorus olitirius L. (COWE) and the content was expressed as 458.13±0.42 mg/100g dry in the 70% ethanol extract of Corchorus olitirius L. (COEE). The antioxidant effects were measured through an experiment evaluating the electron-donating ability and ABTS radical scavenging activities. In order to verify the whitening effects, tyrosinase inhibitory activity was measured and results showed an effect of 11.82% (COWE) and 48.21% (COEE) at 1,000 μg/mL, respectively. An MTT assay was conducted in RAW 264.7 and B16F10 cells to assess the cell survival rate; it was found that no toxicity was apparent at 100 μg/mL. Active suppression of nitric oxide (NO), an inflammation inducer, was measured to verify the anti-inflammatory efficacy. In the NO assay, experiments were conducted using a concentration of 100 μg/mL which led to a 95% survival rate. The NO assay was measured using Griess reagent and compared to the lipopolysaccharide treatment group and non-treatment group. The inhibition of cellular melanin synthesis was measured to determine the whitening effects. At 100 μg/mL, the inhibition activities of COWE and COEE were 15.69% and 22.67%, respectively. It has been confirmed that COWE and COEE are effective antioxidants, have whitening effects, and are anti-inflammatory agents. In particular, the use of COWE and COEE as natural materials for cosmetics was confirmed to have excellent effects.

Abstract

In this study, the effects of the physiological activity of the Corchorus olitirius L. were verified and the applicability as a cosmetic material was reviewed. The total polyphenol content was expressed as 396.44±0.42 mg/100g dry in the water extract of Corchorus olitirius L. (COWE) and the content was expressed as 458.13±0.42 mg/100g dry in the 70% ethanol extract of Corchorus olitirius L. (COEE). The antioxidant effects were measured through an experiment evaluating the electron-donating ability and ABTS radical scavenging activities. In order to verify the whitening effects, tyrosinase inhibitory activity was measured and results showed an effect of 11.82% (COWE) and 48.21% (COEE) at 1,000 μg/mL, respectively. An MTT assay was conducted in RAW 264.7 and B16F10 cells to assess the cell survival rate; it was found that no toxicity was apparent at 100 μg/mL. Active suppression of nitric oxide (NO), an inflammation inducer, was measured to verify the anti-inflammatory efficacy. In the NO assay, experiments were conducted using a concentration of 100 μg/mL which led to a 95% survival rate. The NO assay was measured using Griess reagent and compared to the lipopolysaccharide treatment group and non-treatment group. The inhibition of cellular melanin synthesis was measured to determine the whitening effects. At 100 μg/mL, the inhibition activities of COWE and COEE were 15.69% and 22.67%, respectively. It has been confirmed that COWE and COEE are effective antioxidants, have whitening effects, and are anti-inflammatory agents. In particular, the use of COWE and COEE as natural materials for cosmetics was confirmed to have excellent effects.

참고문헌 (44)

  1. 홍주헌, "생강나무 잎 및 가지 추출물의 생리활성", 한국식품조리과학회지, 29(5), pp.573~580 (2013), 한국식품조리과학회[DOI]
  2. 조은경, "감태 열수 추출물의 생리활성", 생명과학회지, 20(11), pp.1675~1682 (2010), 한국생명과학회
  3. 이연지, "톳(Hizikia fusiformis)을 첨가한 흰찰쌀보리죽의 제조 및 품질특성", 한국수산과학회지, 49(3), pp.310~316 (2016), 한국수산과학회
  4. 이연지, "톳(Hizikia fusiformis) 분말을 첨가한 양갱의 이화학적 품질특성 및 항산화 효과", 한국수산과학회지, 53(4), pp.588~596 (2020), 한국수산과학회[DOI]
  5. 김미지, "중국 여성들의 한국 화장품 선호도 및 브랜드 선호도", 한국인체미용예술학회지, 20(1), pp.227~240 (2019), 한국인체미용예술학회[DOI]
  6. 김미경, "블루베리 잎 추출물의 항산화 및 항염증 효과", 한국인체미용예술학회지, 15(4), pp.109~120 (2014), 한국인체미용예술학회
  7. 장지연, "황칠나무 잎(Dendropanax morbifera. Leaf) 에탄올 추출물의 화장품 소재로서 생리활성 검증", 한국인체미용예술학회지, 21(4), pp.25~37 (2020), 한국인체미용예술학회[DOI]
  8. 김미경, "Neem 에탄올추출물과 분획물의 항산화, 항균 및 항염증 효과 비교", 한국인체미용예술학회지, 22(1), pp.61~74 (2021), 한국인체미용예술학회[DOI]
  9. 이수정, "피부각질형성세포에서 다양한 베리류의 피부노화개선 효과 비교", 한국식품과학회지, 46(2), pp.198~204 (2014), 한국식품과학회[DOI]
  10. 김아름, "목형 잎 추출물의 항산화 활성과 멜라닌 생합성에 대한 저해활성", 한국미생물·생명공학회지, 41(1), pp.135~144 (2013), 한국미생물·생명공학회[DOI]
  11. C. J. Cooksey, "Tyrosinase kinetics: failure of the auto-activation mechanism of monohydric phenol oxidation by rapid formation of a quinomethane intermediate", J. Biochem, 333(Pt3), pp.685~691 (1998)
  12. W. Helen, "Dietary influences on membrane function : importance in protection against oxidative damage and disease", Nutr. Biochem, 7(1), pp.2~15 (1996)
  13. S. Papa, "Reactive oxygen species, mitochondria, apoptosis and aging", Mol. Cell Biochem, 174(1-2), pp.305~319 (1997)
  14. K. N. Kader, "Reactive oxygen and nitrogen species : implications for cardiovascular device engineering", J. Biomed. Mater. Res. B. Appl. Biomater, 83(1), pp.138~144 (2007)
  15. D. A. Willoughby, "Heberden Oration, 1974. Human arthritis applied to animal models. Towards a better therapy", Ann. Rheum. Dis., 34(6), pp.471~478 (1975)
  16. Taddessee Yayeh, "Korean Red Ginseng Saponin Fraction Downregulates Proinfl ammatory Mediators in LPS Stimulated RAW264.7 Cells and Protects Mice against Endotoxic Shock", Journal of Ginseng Research, 36(3), pp.263~269 (2012), 고려인삼학회
  17. M. Yoshikawa, "Medicinal foodstuffs. V. Moroheiya. (1): absolute stereostructures of corchoionosides A, B, and C, histamine release inhibitors from the leaves of Vietnamese Corchorus olitorius L. (Tiliaceae)", Chemi. Pharma. Bulletin, 45(3), pp.464~469 (1997)
  18. A. R. El-Mahdy, "Preliminary studies on the mucilages extracted from Okra fruits, Taro tubers, Jew’s mellow leaves and Fenugreek seeds", Food Chemi, 14(4), pp.237~249 (1984)
  19. A. A. Oshodi, "Comparison of protein, minerals and vitamin C contents of some dried leafy vegetables", Pak. J. Sci. Ind. Res, 35(3), pp.267~269 (1992)
  20. R. S. Farag, "Distribution of carotenoids in some fresh and boiled foods", Adv. Food Sci, 20(1), pp.1~6 (1998)
  21. 정창화, "몰로키아(Corchorus olitorius) 잎에서 추출한 mucilage의 이화학적 특성", 한국식품과학회지, 34(5), pp.757~761 (2002), 한국식품과학회
  22. 김현정, "몰로키아 첨가 탈지대두grit(defatted soybean grit) 발효물의 콜레스테롤 개선 효과", 한국식품과학회지, 43(3), pp.375~380 (2011), 한국식품과학회
  23. 김한수, "몰로키아 분말을 첨가한 밀·보리 혼합 식빵의 제조 및 생리활성 평가", 한국식품영양학회지, 28(4), pp.676~684 (2015), 한국식품영양학회
  24. 한증술, "아열대 엽채류 몰로키아의 생장과 발육 특성", 원예과학기술지, 27(1), pp.49~54 (2009), 한국원예학회
  25. 엄미정, "모로헤이야 파종시기와 적심높이에 따른 신초의 생산성 비교", 생물환경조절학회지, 24(3), pp.196~201 (2015), (사) 한국생물환경조절학회
  26. 김미정, "기능성 화장품 소재로서 몰로키아 추출물의 가능성", 아시안뷰티화장품학술지, 15(1), pp.23~31 (2017), 한국피부과학연구원[DOI]
  27. V. L. Singleton, "Colorimetry of total phenolics with phosphomolybdic:phosphotungstic acid reagents", Am. J. Enol. Viticult, 16, pp.144~158 (1965)
  28. M. S. Blois, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  29. F. Nicoletta, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2, 2'-azinobis(3-ethylben-zothiazoline-6-sulfonic acid)radical cation decolorization assay", Methods Enzymol, 299, pp.379~389 (1999)
  30. A. Yagi, "Inhibition of mushroom-tyrosinase by aloe extract", Planta Med, 53(6), pp.515~517 (1987)
  31. J. Carmichael, "Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing", Cancer Res, 47(4), pp.936~942 (1987)
  32. nitrate in biological fluids, "Anal. Biochem", 1982, 131~138
  33. J. Hosoi, "Regulation of melanin synthesisof B16 melanoma cell by 1 alpha, 25-dihydroxyvitamin D3 and retinoicacid", Cancer Res, 45(4), pp.1474~1478 (1985)
  34. 김소은, "화장품 소재로서 세발나물 추출물의 생리활성 연구", 한국인체미용예술학회지, 20(2), pp.59~71 (2019), 한국인체미용예술학회[DOI]
  35. F. Que, "Antioxidant properties of Chineses yellow wine, its concentrate, and volatiles", LWT-Food Sci. Technol, 39(2), pp.111~117 (2006)
  36. 김옥경, "산수유 추출물이 Streptozotocin으로 유발된 흰쥐의 항당뇨 및 항산화 작용에 미치는 효과", 한국응용과학기술학회지, 22(2), pp.157~167 (2005), 한국응용과학기술학회
  37. 신소림, "자생 양치식물 지상부와 근경 메탄올 추출물의 항산화 효과", 한국자원식물학회지, 23(1), pp.38~46 (2010), 한국자원식물학회
  38. N. Tsuji, "The role of elastases secreted by fibroblast in wrinkle formation : implication through selective inhibition of elastase activity", Photochem Photobiol, 74(2), pp.283~290 (2001)
  39. 고민석, "비타민 나무(Hippophae rhamnoides L.) 열매 추출물의 항산화 활성 및 미백 효과", 동아시아식생활학회지, 22(6), pp.812~817 (2012), 동아시아식생활학회
  40. C. B. Lin, "Modulation of microphthalmia-associated transcription factor gene expression alters skin pigmentation", J. Invest Dermatol, 119(6), pp.1330~1340 (2002)
  41. 안병권, "추출용매에 따른 함초 추출물의 피부미용 효과", 공업화학, 22(1), pp.56~60 (2011), 한국공업화학회
  42. -bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase, "Anal. Biochem", 1997, 206~209
  43. B. P. Madhu, "Role of nitric oxide in the regulation of immune responses during raꠓbies virus infection in mice", Virusdisease, 27(4), pp.387~399 (2016)
  44. J. Y. Kim, "Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages", FEBS Lett, 569(1-3), pp.321~326 (2004)

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