납의 신경독성에 대한 지금초 추출물의 독성경감 효과
Alleviating Effects of Euphorbiae humifusae L. Extract on the Neurotoxicity Induced by Lead
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본 연구는 배양 C6 glioma 세포를 대상으로 초산납(lead acetate, LA)의 신경독성을 알아보았으며, 또한 LA의 세포독성에 대한 지금초(Euphorbiae humifusae L., EH) 추출물의 영향을 조사하였다. 본 연구에서, LA는 대조군에 비하여 농도 의존적으로 세포 생존율이 감소됨으로서 신경독성을 보였으며, 0∼ 50 M의 LA가 각각 포함된 배양액에서 72시간 동안 세포를 처리한 결과, XTT50 값은 38.6 M로 고독성인 것으로 나타났다. 또한, 30∼50 M의 LA가 각각 포함된 배양액에서 72시간 동안세포를 처리한 결과 XTT50은 38.6 M에서 나타났다. 이와 동시에 항산화제인 vitamin E의 처리에서 LA 독성에 의하여 감소된세포 생존율이 유의하게 증가함으로서 LA의 독성에 산화적 손상이 관여하고 있음을 제시하였다. 한편, EH 추출물은 지질과산화 저해능을 비롯하여 superoxide dismutase (SOD)-유사활성능 및 DPPH-라디칼 소거능과 같은 항산화능에 의하여 LA 독성을 효과적으로 방어하였다. 이같은 항산화 효과는 gallic acid와 같은 폴리페놀이나 또는 flavonol이나 quercetin과 같은 플라보노이드와 같은 항산화성분에 기인된 결과로 생각된다. 결론적으로, EH 추출물과 같은 천연 소재는 차후 납신경독성과 같이 산화적 손상과 관련된 질환 치료를 위한 유용한 활용인자로 사료된다.
Abstract
This study examined the neurotoxicity induced by lead acetate (LA) on cultured C6 glioma cells and the protective effects of Euphorbiae humifusae L. (EH) extract against LA-induced cytotoxicity. In this study, LA exhibited neurotoxicity in a dose-dependent manner compared to the control, and was determined to be highly-toxic according to the toxic criteria. The XTT50 value of LA was calculated at a concentration of 38.6 M after C6 glioma cells were incubated for 72 hours in the media containing 30∼50 M of LA, respectively. In addition, LA-induced neurotoxicity was suggested to correlate with the level of oxidative stress because vitamin E, an antioxidant, increased the cell viability damaged by LA-induced cytotoxicity. The EH extract effectively prevented cell injury from LA-induced cytotoxicity via its antioxidative effects, such as inhibitory ability of lipid peroxidation, superoxide dismutase-like activity and 1,1-diphenyl-2-picrylhydrazyl- radical scavenging activity. These antioxidative effects may result in components, such as polyphenol or flavonoids including gallic acid or quercetin. In conclusion, natural resources, such as EH extracts, may be a useful putative agent for the treatment of diseases associated with oxidative stress, such as lead neurotoxicity.
참고문헌 (33)
- Noa Lavid, "The involvement of polyphenols and peroxidase activities in heavy-metal accumulation by epidermal glands of the waterlily (Nymphaeaceae)", Planta, 212(3), pp.323~331 (2001), Springer Nature[DOI]
- Ming-Yie Liu, "In vitro aberrant gene expression as the indicator of lead-induced neurotoxicity in U-373MG cells", Toxicology, 147(1), pp.59~64 (2000), Elsevier BV[DOI]
- Sandra V. Verstraeten, "Aluminium and lead: molecular mechanisms of brain toxicity", Archives of Toxicology, 82(11), pp.789~802 (2008), Springer Nature[DOI]
- 손영우, "환경오염원인 납의 신경독성에 대한 NMDA 수용체 길항제의 보호 효과", 한국산업보건학회지, 27(3), pp.193~200 (2017), 한국산업보건학회[DOI]
- 정재윤, "모발염색제 성분인 초산납에 대한 어성초 추출물의 항산화 및 미백효과", 대한미용학회지, 10(2), pp.13~19 (2014), 대한미용학회[DOI]
- Aleksandra D, "PGC-1 controls mitochondrial biogenesis and dynamics in lead-induced neurotoxicity", J Aging, 7, pp.629~643 (2015)
- 오영희, "꿀풀 추출물이 돌연변이원인 수은독성으로 손상된 배양 NIH3T3 섬유모세포에 미치는 보호 효과", 인간식물환경학회지, 18(1), pp.41~46 (2015), 인간식물환경학회
- Yoo SM, "Effect of phenol compound on the cytotoxicity of heavy metal", J Life Natr Res, 30, pp.80~87 (2008)
- Rosen D, "Mutation in Cu", Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis, 1993, pp.362, 59~62
- 하대호, "메틸수은으로 손상된 골모세포에 대한 NMDA 수용체길항제의 영향", 동의생리병리학회지, 17(2), pp.412~415 (2003), 한의병리학회
- Gracia-Lopez D, "Effects of eccentric exercise on NF-kB activation in blood monolayer cells", Med Sci Sports Exerc, 39, pp.653~664 (2007)
- 정재윤, "치매유발제인 알루미늄으로 손상된 배양 C6 Glioma 세포에 대한 금은화 추출물의 보호 효과", 대한임상검사과학회지, 49(3), pp.271~278 (2017), 대한임상검사과학회[DOI]
- 이진만, "국내 일부 자생식물 추출물의 여드름균 항균활성 탐색", 대한미용학회지, 9(4), pp.46~54 (2013), 대한미용학회[DOI]
- Fujisawa S, "Anti-and pro-oxidant effects of oxidized quercetin curcumin-related compounds with thiols or ascorbate as measured by the induction period method", In Vitro, 20, pp.39~44 (2006)
- Schwartz SM, "Derivation and properties of plate-derived growth factor-independent rat smooth muscle cells", Am J Pathol, 136, pp.1417~1428 (1990)
- Lim KT Park ST, "Neuronal cytotoxicity of oxygen radical in newborn mouse forebrain culture", Kor J Toxicol, 11, pp.187~192 (1995)
- Yu-Jung Cheng, "Enhancement of TNF-α expression does not trigger apoptosis upon exposure of glial cells to lead and lipopolysaccharide", Toxicology, 178(3), pp.183~191 (2002), Elsevier BV[DOI]
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxic assays", J Immunol Meth, 65, pp.55~63 (1983)
- HIROE KIKUZAKI, "Antioxidant Effects of Some Ginger Constituents", Journal of Food Science, 58(6), pp.1407~1410 (1993), Wiley[DOI]
- Marklund S, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.468~474 (1974)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- A.O.A.C, "Official methods of analysis", pp.127~130 (1995), A.O.A.C
- Evelyn Tiffany‐Castiglioni, "Effects of lead on viability and intracellular metal content of c6 rat glioma cells", Journal of Toxicology and Environmental Health, 23(2), pp.267~279 (1988), Informa UK Limited[DOI]
- Borenfreud F, "A simple quantitative procedure using monolayer culture for cytotoxicity assay(HTD", NR-90), 1985, pp.9, 7~9
- Busselberg D, "Blockage of mammalian and invertebrate calcium channels by lead", Neurotoxicol, 14, pp.249~258 (1993)
- 정재윤, "Protective Effect of Ajuga multiflora BUNGE Extract on Lead Toxicity of Environmental Pollutant", 인간식물환경학회지, 20(4), pp.341~350 (2017), 인간식물환경학회[DOI]
- 손영우, "꽃향유 추출물의 배양 신경교종세포에서 모발염색제 성분인 초산납으로 유도된 세포독성 경감 효과", 대한미용학회지, 8(2), pp.115~120 (2012), 대한미용학회[DOI]
- 강성례, "치자와 삼백초 열수추출물의 항산화능 및 콜라겐 합성능", 대한미용학회지, 7(2), pp.165~171 (2011), 대한미용학회[DOI]
- 한승관, "돈육 지방에 미치는 함초(Salicornia herbacea L.)의 항산화 효과", 한국축산식품학회지, 23(1), pp.46~49 (2003), 한국축산식품학회
- Lim JA, "Antiaging ability of methanol extract from Euonymus alatus", J Cosm Pub Health, 3, pp.41~45 (2007)
- Bressler J, "Phospholipid metabolism in neural microvascular endothelial cells after exposure to lead in vitro", Toxicol Appl Pharmacol, 126, pp.352~360 (1994)
- Walid Hamdy El-Tantawy, "Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats", Journal of Traditional and Complementary Medicine, 6(4), pp.327~331 (2016), Elsevier BV[DOI]