Cisplatin의 세포독성에 대한 레몬밤 추출물의 억제 효과
Protective Effect of Lemon balm Extract on Cisplatin-Induced Cytotoxicity
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초록
꿀풀과 (Labiatae)에 속하는 레몬밤 (Mellisa officinalis,Lemon balm) 추출물이 약제성 탈모 유발제인 cisplatin에 미치는 영향을 조사하기 위하여 인체피부섬유모세포(Detroit 551)를 배양한 후 cisplatin에 미치는 레몬밤 추출물의 영향을 항산화(antioxidation) 측면에서 조사하였다. 본 연구에서탈모 유발제인 cisplatin은 배양 Detroit 551세포의 생존율을유의하게 감소시켰으며, XTT50값이 25.7 μM에서 나타났다. 한편, 항산화제인 vitamin E는 cisplatin에 의하여 감소된 세포생존율을 유의하게 증가시킴으로서 cisplatin의 세포독성을 방어하였다. 레몬밤 추출물이 cisplatin에 미치는 영향에있어서, 레몬밤 추출물은 cisplatin만의 처리에 비하여 세포생존율을 유의하게 증가시켰으며, 또한 전자공여능(DPPHradical scavenging activity)과 lactate dehydrogenase (LDH)활성 억제를 나타냄으로서 항산화능을 보였다. 이상의 결과로부터 약제성 모발 유발제인 cisplatin의 세포독성에 산화적 손상이 관여하고 있었으며, 또한 레몬밤 추출물은 항산화능에 의하여 cisplatin의 세포독성을 효과적으로 방어하였다. 따라서, 레몬밤 추출물과 같은 천연물질은 약제성 탈모유발제인 cisplatin과 같이 산화적 손상과 관련이 깊은 물질의 독성을 항산화 측면에서 감소 또는 방어할 수 있는 미용소재로서의 개발적 가치가 크다고 생각된다.
Abstract
The purpose of this study is to evaluate the protective effect of Lemon balm (Mellisa officinalis) extract on the cytotoxicity induced by cisplatin, pharmaceutic alopecia inducer. To achieve this, cisplatin-induced cytotoxicity was assessed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of cisplatin. And also, the effect of vitamin E was examined on the cisplatin-induced cytotoxicity. For the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, Detroit 551 cells were pretreated with 80 or 100 μg/mL of Lemon balm extract for 2 hours, and also, antioxidative effects of Lemon balm extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity and lactate dehydrogenase (LDH) activity were analysed. The results were as follows. Cisplatin showed a significant decrease of cell viability in dose dependently, and the XTT50 value was calculated at 25.7 μM. In the effect of vitamin E, it significantly increased cell viability which were decreased by the cisplatin-induced cytotoxicity. In the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, it significantly increased cell viability which was decreased by cisplatin-induced cytotoxicity, and also it showed the DPPH-radical scavenging activity and LDH inhibitory activity. Therefore, these results suggested that the cytotoxicity of cisplatin may be involved in oxidative stress, and also,Lemon balm extract effectively prevented the cytotoxicity induced by cisplatin via antioxidative effect. Conclusively, the natural extract like Lemon balm may be useful for the development as antioxidative agent via the prevention of the cytotoxicity induced by pharmaceutic alopesia inducer correlated with oxidative stress.
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