화장품 소재 개발을 위한 대두 추출물의 생리활성 평가
Evaluation of Physiological Activity of Soybean Extract for Cosmetic Material Development
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초록
This study was conducted to find cosmetic materials which have fewer side effects and excellent functionality on human skin. In soybean 60% ethanol extract, the scavenging activities of DPPH and ABTS radicals were measured to be 65.78±0.96% and 48.00±0.97%, respectively. The total polyphenol content was measured as 19.42±0.05 mgGAE/g and the total flavonoid content was 13.84±0.40 mgQE/g. In the soybean 60% ethanol extract, the amounts of daidzin and glycitin were 7.459±0.04 mg/g and 2.947±0.04 mg/g, respectively. Furthermore, the survival rate of B16-F10 cell was found to increase as the processing concentration of soybean 60% ethanol extract was increased. Inhibition of tyrosinase enzyme activity was found to be dependent on concentration. The survival rate of CCD-986sk cell was measured and found to be 100% surviving at all concentrations. RAW 264.7 measurements of cell toxicity revealed that cell survival did not reduce at 100-1000 μg/mL concentration. NO generation inhibiting activity reduced the generation of NO with increase in the processing concentration of soybean 60% ethanol extract. The increased processing concentration of soybean 60% ethanol extract also increased TNF-α secretion inhibition, inhibiting the inflammatory reaction by inhibiting the secretion of pro-inflammatory cytokine. The results showed that soybeans are rich in antioxidant, contain many ingredients beneficial to skin health, and can be used as cosmetics materials due to their excellent cell toxicity and physiological activity.
Abstract
This study was conducted to find cosmetic materials which have fewer side effects and excellent functionality on human skin. In soybean 60% ethanol extract, the scavenging activities of DPPH and ABTS radicals were measured to be 65.78±0.96% and 48.00±0.97%, respectively. The total polyphenol content was measured as 19.42±0.05 mgGAE/g and the total flavonoid content was 13.84±0.40 mgQE/g. In the soybean 60% ethanol extract, the amounts of daidzin and glycitin were 7.459±0.04 mg/g and 2.947±0.04 mg/g, respectively. Furthermore, the survival rate of B16-F10 cell was found to increase as the processing concentration of soybean 60% ethanol extract was increased. Inhibition of tyrosinase enzyme activity was found to be dependent on concentration. The survival rate of CCD-986sk cell was measured and found to be 100% surviving at all concentrations. RAW 264.7 measurements of cell toxicity revealed that cell survival did not reduce at 100-1000 μg/mL concentration. NO generation inhibiting activity reduced the generation of NO with increase in the processing concentration of soybean 60% ethanol extract. The increased processing concentration of soybean 60% ethanol extract also increased TNF-α secretion inhibition, inhibiting the inflammatory reaction by inhibiting the secretion of pro-inflammatory cytokine. The results showed that soybeans are rich in antioxidant, contain many ingredients beneficial to skin health, and can be used as cosmetics materials due to their excellent cell toxicity and physiological activity.
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