Melanogenesis-Promoting Effects of Sophora japonica Methanol Extract in Mean-a Cells
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The flowers and flower buds of Sophora japonica are well-known traditional Chinese medicinal herbs. Pharmacologic studies and cases from clinical practices have demonstrated that S. japonica has anti-tumor, anti-fertility, and anti-cancer activities. The active constituents of the herb are also known to be the flavonoid components. The melanogenic effects of S. japonica methanol extract (SJME) were evaluated in melan-a cells, which are immortalized cells derived from C57BL/6 mice. The maximum permissible level of SJME in melan-a cells was over 200 μg/mL. Additionally, SJME stimulated melanin synthesis, as well as tyrosinase activity, in a dose-dependent manner. Furthermore, the melanogenic effect of SJME was greater than that of IBMX, a well-known pigmenting agent. Lastly, SJME treatments at concentrations between 6.25 and 50 μg/mL increased tyrosinase messenger RNA expression. Collectively, these results suggested that SJME enhanced melanogenesis through an interaction with tyrosinase at the transcriptional level.
Abstract
The flowers and flower buds of Sophora japonica are well-known traditional Chinese medicinal herbs. Pharmacologic studies and cases from clinical practices have demonstrated that S. japonica has anti-tumor, anti-fertility, and anti-cancer activities. The active constituents of the herb are also known to be the flavonoid components. The melanogenic effects of S. japonica methanol extract (SJME) were evaluated in melan-a cells, which are immortalized cells derived from C57BL/6 mice. The maximum permissible level of SJME in melan-a cells was over 200 μg/mL. Additionally, SJME stimulated melanin synthesis, as well as tyrosinase activity, in a dose-dependent manner. Furthermore, the melanogenic effect of SJME was greater than that of IBMX, a well-known pigmenting agent. Lastly, SJME treatments at concentrations between 6.25 and 50 μg/mL increased tyrosinase messenger RNA expression. Collectively, these results suggested that SJME enhanced melanogenesis through an interaction with tyrosinase at the transcriptional level.
참고문헌 (32)
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