B16F10 세포에서 쇠별꽃 메탄올추출물의 멜라닌 생성 촉진 효능
Promoting Efficacy of Stellaria aquatica L. Methanol Extract on Melanin Production in B16F10 Cells
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초록
The present study investigated into the efficacy of Stellaria aquatica L. methanol extract (SAME) on melanin production and photoprotection in B16F10 cells irradiated with or without UVA and elucidated the associated molecular and cellular mechanisms. The melanin content of B16F10 cells treated with 25 μg/mL of SAME and 3-isobutyl-1-methylxanthine (IBMX) was found to increase by 18.0% and 37.2%, respectively. On the other hand, tyrosinase activities of B16F10 cells treated with 1,000 μg/mL of SAME and IBMX increased by 35.2% and 27.8%, respectively. Additionally, the levels of reactive oxygen species (ROS) in B16F10 cells irradiated with 5 mJ/cm2 UVA increased by 66.1% (p<0.001). By contrast, when 5 mJ/cm2 UVA-irradiated B16F10 cells were treated with 25 μg/ mL SAME and IBMX the ROS production decreased by 10.8% (p<0.001) and 10.4% (p<0.001), respectively. Taken together, the melanin production-promoting and photoprotective efficacy of SAME in B16F10 cells appeared to be mediated through an increase in tyrosinase activity and a decrease in ROS production in these cells.
Abstract
The present study investigated into the efficacy of Stellaria aquatica L. methanol extract (SAME) on melanin production and photoprotection in B16F10 cells irradiated with or without UVA and elucidated the associated molecular and cellular mechanisms. The melanin content of B16F10 cells treated with 25 μg/mL of SAME and 3-isobutyl-1-methylxanthine (IBMX) was found to increase by 18.0% and 37.2%, respectively. On the other hand, tyrosinase activities of B16F10 cells treated with 1,000 μg/mL of SAME and IBMX increased by 35.2% and 27.8%, respectively. Additionally, the levels of reactive oxygen species (ROS) in B16F10 cells irradiated with 5 mJ/cm2 UVA increased by 66.1% (p<0.001). By contrast, when 5 mJ/cm2 UVA-irradiated B16F10 cells were treated with 25 μg/ mL SAME and IBMX the ROS production decreased by 10.8% (p<0.001) and 10.4% (p<0.001), respectively. Taken together, the melanin production-promoting and photoprotective efficacy of SAME in B16F10 cells appeared to be mediated through an increase in tyrosinase activity and a decrease in ROS production in these cells.
참고문헌 (23)
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