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金銀花 열수 추출물이 피부 美白에 미치는 영향

Effects of Hot Water Extracts from Lonicera Japonica Flos Extracts on whitening using B16F10 cell lines

최정화|이유림|박수연|정민영|김혜화|우배언
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2017.05|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제30권 제2호|pp. 38-50|DOI http://dx.doi.org/10.6114/jkood.2017.30.2.038

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키워드

WhiteningLonicerae Flos ExtractsLFEB16F10 cells
주제분류한의학

초록

Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines. Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression. Results : In results, more than 2000㎍/㎖ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than 2000㎍/㎖ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in 1000㎍/㎖ of LFE treated group. 1,000㎍/㎖ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. 500㎍/㎖ of LFE treated group and 1,000 ㎍/㎖ of LFE treated group was significantly increased. Tyrosinase mRNA expression of α-MSH and LFE 250㎍/㎖ treated group significantly decreased compared to α-MSH treated group. Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening

Abstract

Objectives : This study was designed to investigate effects of Lonicera Flos Extracts(LFE) on whitening using B16F10 cell lines. Methods : In this experiment, we observed effects of LFE on cell viability, inhibitory effects of melanin synthesis, inhibitory effect on tyrosinase, tyrosinase activity, superoxide dismutase(SOD)-like activity and mRNA expression. Results : In results, more than 2000㎍/㎖ of LFE treated group showed lowered cell viability rates significantly compared to albutin treated group. More than 2000㎍/㎖ of LFE treated groups were lower levels of melanin synthesis. Inhibitory effects of melanin production showed in 1000㎍/㎖ of LFE treated group. 1,000㎍/㎖ of LFE treated group significantly suppressed tyrosinase activities in vitro. LFE and albutin treated group significantly decreased tyrosinase activity compared to non treated group. SOD-like activity of LFE treated group was lower than vitamin C treated group but increased depending on concentration. 500㎍/㎖ of LFE treated group and 1,000 ㎍/㎖ of LFE treated group was significantly increased. Tyrosinase mRNA expression of α-MSH and LFE 250㎍/㎖ treated group significantly decreased compared to α-MSH treated group. Conclusions : These results suggest that LFE can inhibit melanin synthesis through inhibitory action on tyrosinase activity. And LFE suppressed tyrosinase activities B16F10 cells significantly. So I suggest LFE can apply to whitening

참고문헌 (46)

  1. Kim GY, "Esthetic" (1997), Soomunsa
  2. Fisher G, "Mechanisms of Photoaging and Chronological Skin aging", Arch Dermatol, 138(11), pp.1462~1470 (2002)
  3. Chung JH, "Differential effects of photoaging Vs intrinsic aging on the Vascularization of human skin", Arch Dermatol, 138(11), pp.1437~1442 (2002)
  4. Wlaschek M, "Solar UV irradiation and dermal photoaging", J Photochem Photobiol B, 63(1), pp.41~51 (2001)
  5. Krutmann J, "New developments in photoprotection of human skin", Skin Pharmacol Physiol, 14(6), pp.401~407 (2001)
  6. Bensky D, "Chinese herbal medicine: materia medica" (1993), Eastland Press
  7. Huang KC, "The Pharmacology of Chinese herbs" (1998), CRC press
  8. Kim DH, "Pharmacology of Oriental medicine" (2005), Sinilsangsa
  9. Kim IR, "Herbal medicine" (2007), Younglimsa
  10. 배지현, "식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과", 한국식품과학회지, 37(4), pp.642~647 (2005), 한국식품과학회
  11. Ohata S, "Protective effects of Taiwan crude drugs on experimental liver injuries", Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan, 113(12), pp.870~880 (1993)
  12. Rim BM, "Effects of Lonicera japonica extract as a biological response modifier", Envirin. Mut. Car, 12(1), pp.45~54 (1992)
  13. Luo ZH, "The combined modulating effects of cerium nitrate with certain Chinese traditional drugs on altered cell-mediated immunities in scald mice", Zhonghua Wai Ke Za Zhi, 28(9), pp.562~575 (1990)
  14. 주정석, "소회향과 금은화 추출물의 급성췌장염에 대한 예방효과", 대한본초학회지, 25(4), pp.39~45 (2010), 대한본초학회
  15. Seo SH, "The Protective effect of Lonicera Japonica Flos extracts on UVB or H2O2-induced human keratinocyte, HaCaT damage", pp.2014
  16. Herbology Editorial Committee of Korean Medicine schools, "Herbology", pp.240~242 (2010), Younglimsa
  17. Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1α. 25-Dihydroxyvitamin D3 and Retinoic Acid.", Cancer Res, 45(4), pp.1474~1478 (1985)
  18. Yagi A, "The effect of tyrosinase inhibition for aloe", Planta Medica, 3981(3981), pp.517~519 (1986)
  19. Martínez‐Esparza M, "Mechanisms of melanogenesis inhibition by tumor necrosis factor‐α in B16", F10 mouse melanoma cells, 1998, pp.255(1), 139~146
  20. Marklund G, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J BioChem, 47, pp.468~474 (1974)
  21. Lee YS, "Clinical dermatology", pp.210~ (1987), Yeomoongak
  22. Publishing committee of Korean Dermatological Association, "Dermatology" (1994), Yeomoongak
  23. Kim JD, "Skin management", pp.23~113 (1994), Gomoonsa
  24. Kim BH, "New cosmetology", pp.73~311 (1998), Yangsaengeuihaksa
  25. Eun HC, "Dermatologic Immunology", pp.147~148 (1999), Publishing department of Seoul university
  26. Schröder JM, "Cytokine Networks in the Skin", J Investig Dermatol, 105, pp.S20~S24 (1995)
  27. 최주호, "구기자 추출물이 피부 미백 및 주름에 미치는 영향", 한방안이비인후피부과학회지, 27(1), pp.58~67 (2014), 대한한방안이비인후피부과학회
  28. 박진미, "오배자의 항 주름 효과 및 미백 효과에 미치는 실험적 연구", 한방안이비인후피부과학회지, 23(1), pp.135~148 (2010), 대한한방안이비인후피부과학회
  29. 안재형, "건칠의 항주름 효과와 미백효과 연구", 한방안이비인후피부과학회지, 29(3), pp.124~133 (2016), 대한한방안이비인후피부과학회[DOI]
  30. 안뜰에봄, "In Vitro Cytotoxicity, Skin Regeneration, Anti-wrinkle, Whitening and In Vivo Skin Moisturizing Effects of Oncheongeum", 대한한방부인과학회지, 29(1), pp.14~34 (2016), 대한한방부인과학회[DOI]
  31. Park HS, "The effect of whitening by Gamiguibitang", J Korean Med Ophthalmol Otolaryngol Dermatol, 17(2), pp.48~58 (2004)
  32. Kim SK, "Experimental Studies on the Depigmental Function of Sabaeck-san", pp.2000
  33. Lee SH, "Depigmenting activity of mapoongko and ephedrae herba", pp.2001
  34. Choi SY, "Inhibitory Effects on Melanin Production of Resveratrol and Stilbene Derivative", pp.2001
  35. Son DS, "Depigmentation activity of Kakamseosiokyong-san", J Korean Med Ophthalmol Otolaryngol Dermatol, 15(2), pp.104~117 (2002)
  36. 김용, "금은화에 대한 최근 10년의 연구 동향", 한의학연구소 논문집, 19(1), pp.17~23 (2010), 한의학연구소
  37. Hong WS, "Profreading of Hwangjenaegyungsomun" (1984), Oriental medicine research institute
  38. Lee HG, "Oriental medicine of lung system internal medicine" (1988), Minseochulpansa
  39. Kim WH, "Theory of Korean medicine", pp.104~107 (1982), Sungbosa
  40. Wang K, "Cosmetic application of selected traditional Chinese herbal medicines", J Ethnopharmacol, 106(3), pp.353~359 (2006)
  41. Sulaimon S, "The Biology of Melanocytes", Vet Dermatol, 14(2), pp.57~65 (2003)
  42. Prota G, "Recent advances in the chemistry of melanogenesis in mammals", J Invest Dermatol, 75(1), pp.122~127 (1980)
  43. 이진영, "도화(Prunus persica Flos) 분획물의 미백 및 주름개선 효과", 한국미생물·생명공학회지, 40(4), pp.364~370 (2012), 한국미생물·생명공학회[DOI]
  44. Gupta AK, "The treatment of melasma ; a review of clinical trials", J Am Acad Dermatol, 55(6), pp.1048~1065 (2006)
  45. Ando S, "Tyrosinase gene transcription and its control by melanogenic inhibitors", J Invest Dermatol, 100(2), pp.150~155 (1993)
  46. Han DS, "Development of similar active material of SOD and Functional Food", Food technique, 7(4), pp.41~49 (1994)

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