연잎 추출물과 연잎 발효 추출물의 항산화 및 항염 효과 비교
Comparison Study of Antioxidant and Anti-inflammatory Effects for Raw or Fermented Lotus (Nelumbo nucifera) Leaf Extracts
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study tended to identify the potential as functional natural materials for soothing of skin and scalp using lotus leaf hot water extract (Nelumbo nucifera hot water extract, NHWE) and fermented lotus leaf extract (fermented nelumbo nucifera leaf extract, FNLE). The antioxidant evaluations such as total polyphenol and flavonoid content, DPPH electron-donating ability, and ABTS radical scavenging ability, were measured. Cell survival rate was measured in Raw 264.7 cells treated with LPS to check the toxicity, and the inhibitory rate of NO-producing and TNF-α and COX-2 protein expression were measured in Raw 264.7 cells after treatment of LPS in order to evaluate the efficacy of anti-inflammatory effect. NHWE and FNLE showed 182.2 mg/g and 103.3 mg/g each in total polyphenol contents, and 26.8 mg/g and 49.1 mg/g each in flavonoid content. As for ABTS radical scavenging ability, high levels of antioxidants (86.6% and 93.7% in NHWE and FNLE) appeared at the concentration of 1000 μg/mL. In DPPH electron-donating ability, NHWE and FNLE showed 66.1% and 73.3% at 1000 μg/mL. FNLE had more antioxidant ability than NHWE. In anti-inflammatory effects, inhibition rate of NO production by LPS increased in a concentration-dependent manner at both NHWE and FNLE, and TNF-α and COX-2 expressions were also decreased by treatment of NHWE and FNLE. In particular, 200 μg/mL of FNLE showed the best anti-inflammatory effect. In summary, fermented lotus leaf extract showed good efficacy in antioxidant and anti-inflammatory tests, and its potential as a functional ingredients was confirmed. It is expected that not only the soothing effect of the scalp and skin, but also the possibility of application as a functional food can be expanded, and it is considered to be suitable as a natural functional raw material.
Abstract
This study tended to identify the potential as functional natural materials for soothing of skin and scalp using lotus leaf hot water extract (Nelumbo nucifera hot water extract, NHWE) and fermented lotus leaf extract (fermented nelumbo nucifera leaf extract, FNLE). The antioxidant evaluations such as total polyphenol and flavonoid content, DPPH electron-donating ability, and ABTS radical scavenging ability, were measured. Cell survival rate was measured in Raw 264.7 cells treated with LPS to check the toxicity, and the inhibitory rate of NO-producing and TNF-α and COX-2 protein expression were measured in Raw 264.7 cells after treatment of LPS in order to evaluate the efficacy of anti-inflammatory effect. NHWE and FNLE showed 182.2 mg/g and 103.3 mg/g each in total polyphenol contents, and 26.8 mg/g and 49.1 mg/g each in flavonoid content. As for ABTS radical scavenging ability, high levels of antioxidants (86.6% and 93.7% in NHWE and FNLE) appeared at the concentration of 1000 μg/mL. In DPPH electron-donating ability, NHWE and FNLE showed 66.1% and 73.3% at 1000 μg/mL. FNLE had more antioxidant ability than NHWE. In anti-inflammatory effects, inhibition rate of NO production by LPS increased in a concentration-dependent manner at both NHWE and FNLE, and TNF-α and COX-2 expressions were also decreased by treatment of NHWE and FNLE. In particular, 200 μg/mL of FNLE showed the best anti-inflammatory effect. In summary, fermented lotus leaf extract showed good efficacy in antioxidant and anti-inflammatory tests, and its potential as a functional ingredients was confirmed. It is expected that not only the soothing effect of the scalp and skin, but also the possibility of application as a functional food can be expanded, and it is considered to be suitable as a natural functional raw material.
참고문헌 (20)
- Bilal M, "The Beast of Beauty : Environmental and Health Concerns of Toxic Components in Cosmetics", Cosmetics, 7(1), pp.13~ (2020)[DOI]
- Blois MS., "Antioxidant Determinations by the Use of a Stable Free Radical", Nature, 181, pp.1199~1200 (1958)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Guha M, "LPS induction of gene expression in human monocytes", Cellular Signal, 13, pp.85~94 (2001)
- Juliano C, "Cosmetic Functional Ingredients from Botanical Sources for Anti-Pollution Skincare Products", Cosmetics, 5(1), pp.19~ (2018)[DOI]
- Kaewkroek K, "Anti-inflammatory Mechanisms of Compounds from Curcuma Mangga Rhizomes using RAW264.7Macrophage Cells", Nat Prod Commun, 5(10), pp.1547~1550 (2010)
- 김경숙, "천연추출물을 이용한 발모 및 두피 개선에 관한 연구 동향", 아시안뷰티화장품학술지, 12(1), pp.17~24 (2014), 한국피부과학연구원
- Kim DW, "Protective effect of leaf extracts from Nelumbo nucifera on DNA damage against oxidative stress", J Appl Oriental Medicine, 10(2), pp.33~40 (2010)
- Kulkarni M, "Attenuation of acute and chronic restraint stress-induced perturbations in experimental animals by Nelumbo nucifera Gaertn", Indian J Pharm Sci, 70(3), pp.327~332 (2008)
- 이초은, "RAW 264.7 대식세포와 염증유도 동물모델에서 산겨릅나무 추출물의 항산화 및 항염증 효과", 한국식품영양과학회지, 46(1), pp.1~9 (2017), 한국식품영양과학회
- 이경석, "고지방 식이와 병행 섭취한 연잎 추출물이 흰쥐의 혈청 및 간 조직 지질 함량에 미치는 영향", 한국식품영양과학회지, 40(11), pp.1544~1547 (2011), 한국식품영양과학회
- Hyun-Sun Lee, "Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care Anti-Aging Ingredient: In Vitro and Animal Study", Journal of Medicinal Food, 15(11), pp.1015~1023 (2012), 한국식품영양과학회[DOI]
- 이은주, "연잎, 연자육, 연자방 에탄올 추출물의 항염증 활성", 생명과학회지, 29(4), pp.436~441 (2019), 한국생명과학회[DOI]
- Lowry OH, "Protein measurement with the Folin phenol reagent", Journal of biological chemistry, 193, pp.265~275 (1951)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharm, 71, pp.109~114 (2000)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Ryu JH, "Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages", Phytother Res, 17, pp.485~489 (2003)
- 우현심, "LPS로 유도된 RAW264.7 대식세포에 대한헛개나무(Hovenia dulcis) 추출물의 항염증 효과", 한국자원식물학회지, 31(5), pp.466~477 (2018), 한국자원식물학회[DOI]
- Wu MJ, "Antioxidant Activity of Methanol Extract of the Lotus Leaf (Nelumbo nucifera Gertn.)", Am J Chin Med, 31(05), pp.687~698 (2003)
- Zhou Y, "Free radical stress in chronic lymphocytic leukemia cells and its role in cellular sensitivity to ROS-generating anticancer agents", Blood, 101(10), pp.4098~4104 (2003)