미용 소재 개발을 위한 연잎의 항산화 효과 탐색
Screening of Antioxidative Activities from Nelumbinis Folium for using of Beauty Materials
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Nelumbo nucifera (Lotus, N. nucifera) is a popular aqueous plant in Eastern Asia. Leaves (Nelumbinis Folium) of N. nucifera are traditionally used to disperse body heat during the summer and is said to increase essential body energies, according traditional Oriental Medicine, and are becoming popular as ingredient of healthy tea. In the recent years, since many synthetic antioxidants have been shown to have side effects, there has been an increase of interest in natural products as antioxidants in scavenging free radicals. In the present study, we first analyzed composition of Nelumbinis folium (NF), and evaluated the possible antioxidative activities of it’s ethanol extracts. Therefore, we measured the effects of NF on scavenging activities against DPPH radical, hydroxyl radical, superoxide radical and hydrogen peroxide. Consequently, 10 μg/mL of ethanol extract from NF exhibited higher scavenging activity coefficient than that of BHT against all. These results indicate that NF protects oxidative activities through scavenging of free radials.
Abstract
Nelumbo nucifera (Lotus, N. nucifera) is a popular aqueous plant in Eastern Asia. Leaves (Nelumbinis Folium) of N. nucifera are traditionally used to disperse body heat during the summer and is said to increase essential body energies, according traditional Oriental Medicine, and are becoming popular as ingredient of healthy tea. In the recent years, since many synthetic antioxidants have been shown to have side effects, there has been an increase of interest in natural products as antioxidants in scavenging free radicals. In the present study, we first analyzed composition of Nelumbinis folium (NF), and evaluated the possible antioxidative activities of it’s ethanol extracts. Therefore, we measured the effects of NF on scavenging activities against DPPH radical, hydroxyl radical, superoxide radical and hydrogen peroxide. Consequently, 10 μg/mL of ethanol extract from NF exhibited higher scavenging activity coefficient than that of BHT against all. These results indicate that NF protects oxidative activities through scavenging of free radials.
참고문헌 (33)
- 고병섭, "In vitro에서 조릿대, 연근과 연잎이 인슐린 작용 및 분비에 미치는 영향", 한국식품과학회지, 38(1), pp.114~120 (2006), 한국식품과학회
- 김성빈, "고지혈증 및 동맥경화 감응 Bio F1B 햄스터에서 연잎(Nelumbo nucifera)의 지질저하 효과", 생약학회지, 36(3), pp.229~234 (2005), 한국생약학회
- 박종철, "연잎에서 DPPH 라디칼 소거활성을 가지는 페놀성 화합물의 분리", 생약학회지, 38(3), pp.263~269 (2007), 한국생약학회
- 우원식, "천연물화학연구법", pp.13~15 (1996), 서울대학교출판부
- 박성규, "연잎, 연꽃 및 연꽃 수술 추출물의 Tyrosinase 활성억제 및 Melanin 생성억제에 의한 미백 효과", 대한본초학회지, 22(4), pp.87~94 (2007), 대한본초학회
- 정보섭, "도해 향약대사전(식물편)" (1990), 영림사
- 허 준, "원전동의보감", pp.710~ (2004), 남산당
- A.O.A.C, "Official Methods of Analysis" (1990), Association of official analytical chemists
- Beauchamp C, "Superoxide dismutase: improved assays and an assay applicable to acrylamide gels", Anal Biochem, 44(1), pp.276~287 (1971)
- Blosi MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Cho EJ, "Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical", Phytomedicine, 10, pp.544~551 (2003)
- Du H, "Antioxidant and hepatic protective effects of lotus root hot water extract with taurine supplementation in rats fed a high fat diet", Journal of Biomedical Science, 24, pp.17~39 (2010)
- Fridovich, I, "Superoxide dismutase", J Biol Chem, 264, pp.7761~7764 (1989)
- Furukawa H, "On the alkaloids of Nelumbo nucifera Gaertn. alkaloids of Loti Embryo", Yakugaku Zasshi, 86, pp.75~77 (1966)
- Gulcin I, "Antioxidant and analgesic activities of turpentine of Pinus nigra Arn.subsp. pallsiana (Lamb.) Holmboe", J Ethnopharmacol, 86(1), pp.51~58 (2003)
- Halliwell B, "Free radicals in biology and medicine" (1998), Oxford University Press
- Huang B, "Comparative analysis of essential oil components and antioxidant activity of extracts of Nelumbo nucifera from various areas of China", J Agric Food Chem, 58(1), pp.441~448 (2010)
- Jiang Y, "First isolation of tryptophan from edible lotus (nelumbo nucifera Gaertn) rhizomes and demonstration of its antioxidant effects", Int J Food sci & Nutr, 61(4), pp.346~356 (2010)
- Kashiwada Y, "Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera and structure. activity correlations with related alkaloids", Bioorg Med Chem, 13, pp.443~448 (2005)
- L’abbe M, "Changes in pancreatic glutatione peroxidase and superoxide dismutase activities in the prediabetic diabeticprone rat", Proc Soc Exp Biol Med, 207(2), pp.206~212 (1994)
- Matthew B, "Reactive oxygen species in immune responses", Free Radic Biol Med, 36(12), pp.1479~1480 (2004)
- Neyens E, "A review of classic Fenton’s peroxidation as an advanced oxidation technique", J Hazard Mater, 98, pp.33~35 (2003)
- Ono Y, "Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats", J Ethnopharmacol, 106, pp.238~244 (2006)
- Rai S, "Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds", J Ethnopharmacol, 104(3), pp.322~327 (2006)
- Saito, Yamanaka N, "Glutathione-related detoxication function in streptozotocin-induced diabetic rats", J Vet Med Sci, 55(6), pp.991~994 (1993)
- Siegner R, "Lotus leaf extract and Lcarnitine influence different processes during the adipocyte life cycle", Nutr Metab, 7, pp.66~ (2010), Winnefeld M:Lotus leaf extract and Lcarnitine influence different processes during the adipocyte life cycle.Nutr Metab 7:66
- Sies H, "Oxidative Stress: From basic research to clinical application", Am J Med, 91(3C), pp.31S~38S (1991)
- Vergani L, "Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines", Eur J Biochem, 271(18), pp.3646~3656 (2004)
- Wu CH, "Improvement in high-fat diet-induced obesity and body fat accumulation by a Nelumbo nucifera leaf flavonoid-rich extract in mice", J Agric Food Chem, 58(11), pp.7075~7081 (2010)
- Wu MJ, "Antioxidant activity of methanol extract of the lotus leaf (Nelumbo nucifera Gaertn.)", Am J Chin Med, 31(5), pp.687~698 (2003)
- Yan SL, "Determination of catechin in lotus rhizomes by high-performance liquid chromatography", Int J Food Sci Nutr, 28, pp.1~7 (2009)
- Yang D, "Antioxidant activities of various extracts of lotus (Nelumbo nuficera Gaertn) rhizome", Asia Pac J Clin Nutr, 16(1), pp.158~163 (2007)
- "식품의약품안전청 건강기능식품정보 기능성원료 현황" (2010)[URL]
