치과 임플란트용 Ti-6Al-4V 합금 표면의 나노튜브 제조 및 세포부착 특성
Fabrication and cell attachment characteristics of nanotubes on the surface of Ti-6Al-4V dental implant alloy
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Purpose: The purpose of this study was to investigate cell attachment characteristics of nanotubes on the surface of Ti-6Al-4V dental implant alloy. Materials and Methods: TiO2 nanotubes were formed on the Ti-6Al-4V alloys by anodic oxidation. The electrolyte solution was prepared by adding H3PO4 solution to 0.8 wt% NaF solution and then stirred with a magnetic stirrer. With a platinum electrode and a saturated calomel electrode, electrochemical experiments were performed using a conventional three-electrode configuration. Anodic oxidation was carried out using a scanning potentiostat, and all experiments were proceeded as planed at room temperature. Crystallization treatment of nanotube surface went off at 450oC for 3 hours. MC3T3-E1 cell culture was performed with MC3T3-E1 mouse osteoblast for 2 days. Results: Microstructure of the alloys and nanotube surface were observed by optical microscope, field emission scanning electron microscope, and crystallization of nanotube was identified by X-ray diffractometer. Conclusion: The formation of the nanotube structure on the Ti-6Al-4V alloy significantly increased the surface area, which could be useful for the adsorption and adhesion enhancements of cells and could be applied for drug delivery system.
Abstract
Purpose: The purpose of this study was to investigate cell attachment characteristics of nanotubes on the surface of Ti-6Al-4V dental implant alloy. Materials and Methods: TiO2 nanotubes were formed on the Ti-6Al-4V alloys by anodic oxidation. The electrolyte solution was prepared by adding H3PO4 solution to 0.8 wt% NaF solution and then stirred with a magnetic stirrer. With a platinum electrode and a saturated calomel electrode, electrochemical experiments were performed using a conventional three-electrode configuration. Anodic oxidation was carried out using a scanning potentiostat, and all experiments were proceeded as planed at room temperature. Crystallization treatment of nanotube surface went off at 450oC for 3 hours. MC3T3-E1 cell culture was performed with MC3T3-E1 mouse osteoblast for 2 days. Results: Microstructure of the alloys and nanotube surface were observed by optical microscope, field emission scanning electron microscope, and crystallization of nanotube was identified by X-ray diffractometer. Conclusion: The formation of the nanotube structure on the Ti-6Al-4V alloy significantly increased the surface area, which could be useful for the adsorption and adhesion enhancements of cells and could be applied for drug delivery system.
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