본문 바로가기
KERD
논문KCI 등재

한우 급여사료 내 에너지원 사료가 연속식 배양에 따른 반추위 내 미생물 발효특성 및 미생물체 단백질과 아미노산 조성변화에 미치는 영향

Effects of Energy Feed Sources in Hanwoo Cattle Diets on Fermentation and Microbial Amino Acids Composition in a Rumen Simulation Continuous Culture System

정수연|송재용|이지혜|김창현|한지훈|김상윤|배귀석
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2023|농업생명과학연구 (Journal of Agriculture & Life Science)|제57권 제6호|pp. 105-115

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

아미노산한우Hanwoo에너지원 사료연속식 배양Amino acidsContinuous cultureEnergy feeds

초록

한우에게 급여하는 사료 내 에너지원의 종류에 따라 반추위 미생물의 아미노산 조성에 차이가 있는지를 조사하고자 본 연구를 수행하였다. 국내 한우 비육우 사육에 주로 이용되는 에너지원 사료인 옥수수(T1), 생미강(T2), 소맥(T3) 그리고 소맥피(T4)로 반추위 환경과 유사한 연속식 배양기를 이용하여 72시간까지 배양을 진행하였다. 배양이 진행되는 동안 6시간 간격으로 배양액의 발효 성상을 확인하였으며 pH, NH3-N이 일정하게 유지되는 것을 확인하였으며, 배양 종료 시점인 72시간대에 미생물체 단백질 합성량(MPS), 미생물의 아미노산 조성 및 미생물 균총 변화를 분석하였다. 배양액의 pH는 모든 처리구에서 배양 기간동안 5.5~7.0을 유지하였다. Total VFA 농도는 T1이 23.13 mM으로 가장 낮았고, T4가 29.93 mM으로 가장 높았다(p

Abstract

The study investigates whether the composition of amino acids in ruminant microbial, differs according to different energy source feeds used for Korean beef cattle (Hanwoo) finishing diets. This experiment analyzed ruminal fermentation patterns, microbial protein synthesis (MPS), microbial amino acid composition, and the microbial community after 72 h continuous culture in vitro rumen fermentation usingcorn grain (T1), rice bran (T2), wheat grain (T3), and wheat bran (T4). The pH in the culture was maintained at 5.5~7.0 during theincubation in all treatment. The total VFA concentration was highest (29.93 mM) in T4 and lowest (23.13 mM) in T1 (p<0.05). TheA (acetate) :P (propionate) ratio was highest (2.81) in T4 and lowest (1.48) T1 (p<0.05). The butyrate was lowest (2.37 mM) T1 andhighest (4.37~4.58 mM) in T2, T3, T4 (p<0.05). The MPS was highest (332.5 mg/L) T2 and (320.2 mg/L) T3, lowest (137.5 mg/L)T1 and (154.2 mg/L) T4 (p<0.05). The rumen microbial community analysis by DGGE showed a 57.5% similarity or less between treatments. The total amino acid concentrations in rumen microorganisms was highest (31.59 %) T1 and (31.33 %) T3, lowest (20.09 %) T2 (p<0.05). This was difference to the total amino acid concentration in the feed. Therefore, this study confirmed the different energy feeds for Hanwooaffected rumen fermentation patterns, MPS changing the microbial community and amino acid content of rumen microorganisms.

참고문헌 (36)

  1. AOAC, "Official methods of analysis" (2007), Association of Official Analytical Chemists
  2. Bach A, "Nitrogen metabolism in the rumen", J. Dairy. Sci, 88(Suppl), pp.9~21 (2005)
  3. Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
  4. Carberry CA, "Effect of phenotypic residual feed intake and dietary forage content on the rumen microbial community of beef cattle", Appl. Environ. Microbiol, 78(14), pp.4949~4958 (2012)
  5. Chamberlain DG, "Utilization of silage nitrogen in sheep and cows : Amino acid composition of duodenal digesta and rumen microbes", Grass Forage Sci, 41, pp.31~38 (1986)
  6. Chaney AL, "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~ (1962)
  7. Clark JH, "Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows", J. Dairy. Sci, 75(8), pp.2304~2323 (1992)
  8. Clark JH, "Supplying the protein needs of dairy cattle from by-product feeds", J. Dairy. Sci, 70, pp.1092~1109 (1987)
  9. Czerkawski JW, "Design and development of a long-term rumen simulation technique(Rusitec)", Br. J. Nutr, 38, pp.371~384 (1977)
  10. Dehority BA, "Development of, and natural fluctuations in, rumen microbial populations", pp.196~245 (1997), Blackie Academic & Professional
  11. Dehority BA, "Isolation and characterization of several cellulolytic bacteria from in vitro rumen fermentations", J. Dairy. Sci, 46, pp.217~222 (1963)
  12. Dewhurst RJ, "Microbial protein supply from the rumen", Ani. Feed Sci. Tech, 85, pp.1~21 (2000)
  13. Erwin ES, "Volatile fatty acid analyses of blood and rumen fluid by gas chromatography", J. Dairy. Sci, 44, pp.1786~1771 (1961)
  14. Henke A, "Effect of dietary Quebracho tannin extract on feed intake, digestibility, excretion of urinary purine derivatives and milk production in dairy cows", Arch. Anim. Nutr, 71, pp.37~53 (2017)
  15. Mahfuzul Islam, "Recent Application Technologies of Rumen Microbiome Is the Key to Enhance Feed Fermentation", 생명과학회지, 28(10), pp.1244~1253 (2018), 한국생명과학회[DOI]
  16. 김상윤, "한우 급여사료 내 에너지원 사료가 반추위 내 in vitro 미생물발효 특성 및 미생물체 단백질과 아미노산 조성변화에 미치는 영향", 동물자원연구, 33(3), pp.94~104 (2022), 동물생명과학연구소[DOI]
  17. Lu Z, "Dietary energy level promotes rumen microbial protein synthesis by improving the energy productivity of the ruminal microbiome", Front. Microbiol, 10, pp.847~ (2019)
  18. Muyzer G, "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA", Environ. Microbiol, 59(3), pp.695~700 (1993)
  19. National Research Council(NRC), "National science education standards" (1996), The National Academy Press
  20. Nocek JE, "Protein and energy as an integrated system", J. Dairy. Sci, 71, pp.2070~2107 (1988)
  21. Reynolds CK, "Nitrogen recycling through the gut and the nitrogen economy of ruminants : An asynchronous symbiosis", J. Anim. Sci, 86, pp.293~305 (2007)
  22. Rosen H, "A modified ninhydrin colorimetric analysis for amino acids", Arch. Biochem. Biophys, 67, pp.10~15 (1957)
  23. Russell JB, "Effect of pH on the efficiency of growth by pure cultures of rumen bacteria in continuous culture", Environ. Microbiol, 39(3), pp.604~610 (1980)
  24. Russell JB, "Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH? J", Dairy. Sci, 79, pp.1503~1509 (1996)
  25. Satter LD, "Effect of ammonia concentration on rumen microbial protein production in vitro", Br. J. Nutr, 32, pp.199~208 (1974)
  26. Scheifinger CC, "Propionate formation from cellulose and soluble sugars by combined cultures of bacteroides succinogenes and Selenomonas ruminantium", Appl. Microbial, 26(5), pp.789~795 (1973)
  27. Schwab CG, "Rumen protected amino acids for dairy cattle : Progress towards determining lysine and methionine requirements", Anim. Feed. Sci. Tech, 59, pp.87~101 (1996)
  28. 서자겸, "Effects of Synchronicity of Carbohydrate and Protein Degradation on Rumen Fermentation Characteristics and Microbial Protein Synthesis", Animal Bioscience, 26(3), pp.358~365 (2013), 아세아·태평양축산학회[DOI]
  29. Slyter LL, "Influence of acidosis on rumen function", J. Anim. Sci, 43(4), pp.910~929 (1976)
  30. Stern MD, "Effects of nonstructural carbohydrate, urea and soluble protein levels on microbial protein synthesis in continuous culture of rumen content", J. Anim. Sci, 47(4), pp.944~956 (1978)
  31. Storm E, "The nutritive value of rumen micro-organisms in ruminants. 3. The digestion of microbial amino and nucleic acids in, and losses of endogenous nitrogen from, the small intestine of sheep", Br. J. Nutr, 50(2), pp.479~485 (1983)
  32. Tas MV, "The digestibility of amino acids in the small intestine of the sheep", Br. J. Nutr, 45(1), pp.167~174 (1981)
  33. Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition", J. Dairy. Sci, 74(10), pp.3583~3597 (1991)
  34. Wang C, "Effects of different dietary protein levels and rumen-protected folic acid on ruminal fermentation, degradability, bacterial populations and urinary excretion of purine derivatives in beef steers", J. Agri. Sci, 155, pp.1477~1486 (2017)
  35. Yang CT, "Rumen fermentation and bacterial communities in weaned chahaer lambs on diets with different protein levels", J. Integr. Agric, 15, pp.1564~1574 (2016)
  36. Zhang XX, "Reducing protein content in the diet of growing goats : Implications for nitrogen balance, intestinal nutrient digestion and absorption, and rumen microbiota", Animal, 14(10), pp.2063~2073 (2020)

관련 논문AI추천

한우 급여사료 내 에너지원 사료가 반추위 내 in vitro 미생물발효 특성 및 미생물체 단백질과 아미노산 조성변화에 미치는 영향김상윤, 김은중, 김창현, 배귀석, 송재용, 이만희, 이지혜, 정수연동물생명과학연구소 · 동물자원연구2022 · 제33권 제3호한우 급여 단백질 사료원의 반추위 내 미생물 발효에 따른 우회 단백질과 반추위 미생물 아미노산 조성에 미치는 영향김은중, 김창현, 나혜리, 배귀석, 장문백농업생명과학연구원 · 농업생명과학연구2021 · 제55권 제1호한우 육성기 급여 사료 내 Neutral Detergent-Soluble Fiber (NDSF) 함량이 In Vitro 반추위 발효 특성 및 Methane 발생에 미치는 영향강인기, 김상윤, 김창현, 배귀석, 이혜안, 정수연, 최다현, Narantuya Batburged동물생명과학연구소 · 동물자원연구2024 · 제35권 제2호Effects of diets for three growing stages by rumen inocula donors on in vitro rumen fermentation and microbiome강륙석, 박철주, 박탄솔, 이후승, Hyeonsu Seon, Jaeyong Song, Joong Kook Park, Min-Seok Kim, Yong Kwan Kim한국축산학회 · 한국축산학회지2024 · 제66권 제3호Substitution effects of rice for corn grain in total mixed ration on rumen fermentation characteristics and microbial community in vitro김한빈, 문준범, Daekyum Yoo, Jaeyong Song, Jakyeom Seo, Muhammad Mahboob Ali Hamid, Seyoung Lee한국축산학회 · 한국축산학회지2020 · 제62권 제5호Effect of corn grain particle size on ruminal fermentation and blood metabolites of Holstein steers fed total mixed ration박성권, 이성실, 최성호, 최창원, Do Hyung Kim아세아·태평양축산학회 · Animal Bioscience2018 · 제31권 제1호

이 간행물의 다른 논문 (14건)

Catalog of Lactic Acid Bacteria Associated with Vegetable Sprouts손대영, 김진우, 김원일, 이예영, 강용성, 최옥희pp. 1-8논콩 재배에서 근류균 접종 방법과 시기에 따른 콩의 생육과 수량 변화이지현, 정용현, 최나영, 최미리, 나채인pp. 9-18보호자 기질 대상자를 위한 원예 활동 프로그램 구성 전략 도출허무룡, 김용현pp. 19-24초분광 영상을 이용한 콩 들불병 진단을 위한 주요 파장 선정 및 모델 개발김은리, 박진기, 박기수, 류찬석, 정종찬, 강예성pp. 25-38경주국립공원 소금강지구의 관속식물상유주한pp. 39-54
원문 보기