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Catalog of Lactic Acid Bacteria Associated with Vegetable Sprouts

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In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa),clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus),red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences,the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostocmesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species includingE. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activitytoward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failedto suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significantin some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contributeto further LAB research and improved vegetable sprout production.

Abstract

In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa),clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus),red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences,the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostocmesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species includingE. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activitytoward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failedto suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significantin some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contributeto further LAB research and improved vegetable sprout production.

참고문헌 (53)

  1. Abee T, "Microbial stress response in minimal processing", Int. J. Food Microbiol, 50, pp.65~91 (1999)
  2. 안수빈, "Characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, Korea traditional fermented food, for probiotic use", Journal of Biomedical and Translational Research, 14(4), pp.206~211 (2013), 동물의학연구소[DOI]
  3. Albanese A, "Molecular identification of Leuconostoc mesenteroides as a cause of brain abscess in an immunocompromised patient", J. Clin. Microbiol, 44, pp.3044~3045 (2006)
  4. Bang J, "Inactivation of Escherichia coli O157:H7 on radish seeds by sequential treatments with chlorine dioxide, drying, and dry heat without loss of seed viability", Appl. Environ. Microbiol, 77, pp.6680~6686 (2011)
  5. Barak JD, "Differences in attachment of Salmonella enterica serovars and Escherichia coli O157 : H7 to alfalfa sprouts", Appl. Environ. Microbiol, 68, pp.4758~4763 (2002)
  6. Björkroth KJ, "Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples", Int. J. Syst. Evol. Microbiol, 52, pp.141~148 (2002)
  7. Brashears MM, "Antagonistic action of Lactobacillus lactis toward Salmonella spp. and Escherichia coli O157: H7 during growth and refrigerated storage", J. Food Prot, 62, pp.1336~1340 (1999)[DOI]
  8. Breuer T, "A multistate outbreak of Escherichia coli O157:H7 infections linked to alfalfa sprouts grown from contaminated seeds", Emerg. Infect. Dis, 7, pp.977~982 (2001)
  9. Callejon RM, "Reported foodborne outbreaks due to fresh produce in the United States and European Union : Trends and causes", Foodborne Pathog. Dis, 12, pp.32~38 (2015)
  10. Chen YS, "Lactococcus taiwanensis sp. nov., a lactic acid bacterium isolated from fresh cummingcordia", Int. J. Syst. Evol. Microbiol, 63, pp.2405~2409 (2013)
  11. Costa R, "Preservation of meat products with bacteriocins produced by lactic acid bacteria isolated from meat", J. Food Qual, 2019, pp.1~12 (2019)
  12. Diana CR, "Probiotic properties of Leuconostoc mesenteroides isolated from aguamiel of Agave salmiana", Probiotics Antimicrob. Proteins, 7, pp.107~117 (2015)
  13. Elavarasi V, "Screening and characterization of Weissella cibaria isolated from food source for probiotic properties", IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology NCWBCB, pp.29~32 (2014)
  14. "Microbiological safety evaluations and recommendations on sprouted seed" (2023)[URL]
  15. Fett WF, "Inhibition of Salmonella enterica by plant-associated pseudomonads in vitro and on sprouting alfalfa seed", J. Food Prot, 69, pp.719~728 (2006)
  16. Foster JW, "Adaptive acidification tolerance response of Salmonella typhimurium", J. Bacteriol, 172, pp.771~778 (1990)
  17. Foster JW, "Low pH adaptation and the acid tolerance response of Salmonella typhimurium", Crit. Rev. Microbiol, 21, pp.215~237 (1995)
  18. Fusco V, "The genus Weissella : Taxonomy, ecology and biotechnological potential", Front. Microbiol, 6, pp.155~ (2015)
  19. Gänzle MG, "Effect of ecological factors on the inhibitory spectrum and activity of bacteriocins", Int. J. Food Microbiol, 46, pp.207~217 (1999)
  20. Gänzle MG, "Lactic metabolism revisited : Metabolism of lactic acid bacteria in food fermentations and food spoilage", Curr. Opin. Food Sci, 2, pp.106~117 (2015)
  21. Gelfand MS, "Confused by Weissella confusa bacteremia", J. Clin. Microbiol, 53, pp.2001~ (2015)
  22. Hanchi H, "The genus Enterococcus: Between probiotic potential and safety concerns an update", Front. Microbiol, 9, pp.1791~ (2018)
  23. Janssen M, "Individual and combined effects of pH and lactic acid concentration on Listeria innocua inactivation : Development of a predictive model and assessment of experimental variability", Appl. Environ. Microbiol, 73, pp.1601~1611 (2007)
  24. Kamboj K, "Identification and significance of Weissella species infections", Front. Microbiol, 6, pp.1204~ (2015)
  25. Kim WI, "Inhibition of Salmonella enterica growth by competitive exclusion during early alfalfa sprout development using a seed-dwelling Erwinia persicina strain EUS78", Int. J. Food Microbiol, 312, pp.108374~ (2020)
  26. Kulwichit W, "Accuracies of Leuconostoc phenotypic identification: A comparison of API systems and conventional phenotypic assays", BMC Infect. Dis, 7, pp.69~ (2000)
  27. Lee KW, "Probiotic properties of Weissella strains isolated from human faeces", Anaerobe, 8, pp.96~102 (2012)
  28. Lee YJ, "Supplementation with probiotic strain Weissella cibaria JW15 enhances natural killer cell activity in nondiabetic subjects", J. Funct. Foods, 48, pp.153~158 (2018)
  29. LeLièvre V, "Phages for biocontrol in foods: What opportunities for Salmonella sp. control along the dairy food chain?", Food Microbiol, 78, pp.89~98 (2019)
  30. Liao CH, "Growth of Salmonella on sprouting alfalfa seeds as affected by the inoculum size, native microbial load and Pseudomonas fluorescens 2-79", Lett. Appl. Microbiol, 46, pp.232~236 (2008)
  31. Limanska N, "Study of the potential application of lactic acid bacteria in the control of infection caused by Agrobacterium tumefaciens", J. Plant Pathol. Microbiol, 6, pp.1~9 (2015)
  32. Ludlow SP, "A case report of Pediococcus pentosaceus bacteremia successfully treated with daptomycin", Infect. Dis. Clin. Pract, 22, pp.e1~e2 (2014)[DOI]
  33. Márton M, "The role of sprouts in human nutrition. A review", Acta Univ. Sapientiae. Aliment, 3, pp.81~117 (2010)
  34. Matos A, "Effects of community versus single strain inoculants on the biocontrol of Salmonella and microbial community dynamics in alfalfa sprouts", J. Food Prot, 68, pp.40~48 (2005)
  35. Mazzoni AM, "Supercritical carbon dioxide treatment to inactivate aerobic microorganisms on alfalfa seeds", J. Food Saf, 21, pp.215~223 (2001)
  36. McIntyre L, "Biocontrol of foodborne bacteria : Past, present and future strategies", Food New Zealand, 7, pp.25~ (2007)
  37. Minervini F, "Lactic acid bacteria in durum wheat flour are endophytic components of the plant during its entire life cycle", Appl. Environ. Microbiol, 81, pp.6736~6748 (2015)
  38. Nandiwada LS, "Characterization of an E2-type colicin and its application to treat alfalfa seeds to reduce Escherichia coli O157 : H7", Int. J. Food Microbiol, 93, pp.267~279 (2004)
  39. Nei D, "Disinfection of radish and alfalfa seeds inoculated with Escherichia coli O157 : H7 and Salmonella by a gaseous acetic acid treatment", Foodborne Pathog. Dis, 8, pp.1089~1094 (2011)
  40. Pao S, "Use of bacteriophages to control Salmonella in experimentally contaminated sprout seeds", J. Food Sci, 69, pp.M127~M130 (2004)
  41. Paradhipta DHV, "Dual-purpose inoculants and their effects on corn silage", Microorganisms, 8, pp.765~ (2020)
  42. Phan YTN, "Diversity of lactic acid bacteria in vegetable-based and meat-based fermented foods produced in the central region of Vietnam", AIMS Microbiol, 3, pp.61~70 (2017)
  43. Reuter G., "Probiotics-possibilities and limitations of their application in food, animal feed, and in pharmaceutical preparations for men and animals", Berl. Munch. Tierarztl. Wochenschr, 114, pp.410~419 (2001)
  44. Sharma RR, "Application of ozone for inactivation of Escherichia coli O157 : H7 on inoculated alfalfa sprouts", J. Food Process. Preserv, 27, pp.51~64 (2003)
  45. Shen Z, "Biocontrol of the internalization of Salmonella enterica and Enterohaemorrhagic Escherichia coli in mung bean sprouts with an endophytic Bacillus subtilis", Int. J. Food Microbiol, 250, pp.37~44 (2017)
  46. Shukla R, "Probiotic potential of Pediococcus pentosaceus CRAG3: A new isolate from fermented cucumber", Probiotics Antimicrob. Proteins, 6, pp.11~21 (2014)
  47. Smith L, "Reduction of Escherichia coli O157 : H7 and Salmonella in ground beef using lactic acid bacteria and the impact on sensory properties", J. Food Prot, 68, pp.1587~1592 (2005)
  48. Tian JQ, "Survival and growth of foodborne pathogens in minimally processed vegetables at 4 and 15°C", J. Food Sci, 77, pp.M48~M130 (2012)[DOI]
  49. Trias R, "Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi", Int. Microbiol, 11, pp.231~236 (2008)
  50. Wilderdyke MR, "Isolation, identification, and selection of lactic acid bacteria from alfalfa sprouts for competitive inhibition of foodborne pathogens", J. Food Prot, 67, pp.947~951 (2004)
  51. Wuytack EY, "Decontamination of seeds for seed sprout production by high hydrostatic pressure", J. Food Prot, 66, pp.918~923 (2003)
  52. Yang Y, "Overview of recent events in the microbiological safety of sprouts and new intervention technologies", Compr. Rev. Food Sci. Food Saf, 12, pp.265~280 (2013)
  53. 유형석, "Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15", Food Science and Biotechnology, 28(3), pp.851~855 (2019), 한국식품과학회[DOI]

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