본문 바로가기
KERD
논문KCI 등재

이중 중합효소연쇄반응을 이용한 거대세포바이러스 검출

Detection of Cytomegalovirus by Dual-PCR

안유헌|강정옥|최태열|최원호
대한임상미생물학회 (Korean Society of Clinical Microbiology)|2006.10|Annals of Clinical Microbiology|제9권 제2호|pp. 96-101

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

Polymerase chain reactionCytomegalovirusLate antigen (LA) geneMajor Immediate Early Antigen (MIE) geneAntigenemia

초록

배경：Cytomegalovirus (CMV) 감염은 장기이식 환자뿐만 아니라 면역 저하 환자의 이환 및 사망의 중요한 원인이다. 연구자들은 혈액 검체에서 CMV를 검출하기 위한 이중-중합효소연쇄반응법과 항원 혈증 검사의 결과를 비교하였다. 방법：한양대학병원에서 2002년 2월부터 2005년 5월까지 CMV 검출을 위하여 의뢰된 175개 혈액 검체 결과를 분석하였다. Dual-PCR은 CMV의 late antigen (LA) 및 major immediate early antigen (MIE) 유전자를 동시에 증폭하였고, 항원혈증 검사(Chemicon, Temecula, CA, USA)는 CMV의 lower matrix pp65 항원을 검출하였다. 결과：Dual-PCR의 양성율은 14.3% (25/175), 항원혈증 검사는 13.1% (23/175)이었다. Dual-PCR과 항원혈증 검사의 일치율은 85.1%(149/175), 불일치율은 14.9% (26/175)이였다. 결론：Dual-PCR 검사는 CMV의 조기 검출에 유용한 방법이나, 두 방법간에 불일치율이 높아 항원 혈증 검사와 함께 사용할 것을 추천한다.

Abstract

Background: Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality in transplant recipients and immunocompromised patients. We compared the results of a dual polymerase chain reaction (dual-PCR) and an antigenemia (Ag) test for detection of CMV from blood samples. Methods: Between February 2002 and May 2005, we analyzed 175 blood samples submitted for CMV tests at Hanyang University Hospital. The late antigen (LA) and major immediate early (MIE) genes of CMV were concurrently amplified in the dual-PCR. The lower matrix protein pp65 of CMV was detected for the Ag test (Chemicon, Temecula, CA, USA). Results: The positive rate of the dual-PCR was 14.3% (25/175) and that of the Ag test was 13.1% (23/175). The concordance rate of the dual-PCR and Ag test was 85.1% (149/175), while the discordance rate was 14.9% (26/175). Conclusion: The dual-PCR is a useful method for the early detection of CMV, but we recommend using both the dual-PCR and Ag test for detection of CMV due to a high discordance rate of the two methods.

참고문헌 (29)

  1. "Quantitation of cytomegalovirus DNA by end-point titration and dual polymerase chain reaction in renal transplant recipients", 10, pp.25~33 (1996)
  2. "Comparison of polymerase chain reaction and viral culture for the detection of cytomegalovirus", 13, pp.607~16 (1993)
  3. "Comparison of polymerase chain reaction method and CMV antigenemia assay for diagnosis of cytomegalovirus infection in transplanted patients", 2, pp.177~181 (1998)
  4. "The cytomegalovirus-antigenemia assay in the diagnosis of posttransplant cytomegalovirus infection", 4, pp.1615~22 (1994)
  5. "Polymerase chain reaction to evaluate antiviral therapy for cytomegalovirus disease", 338, pp.1170~2 (1991)
  6. "Polymerase chain reaction assay for detection of human cytomegalovirus", 27, pp.1238~42 (1989)
  7. "The usefulness of PCR and early antigen immunostaining as a rapid identification method of cytomegalovirus infection", 18, pp.452~457 (1998)
  8. "Semiquantitative PCR for detection of human cytomegalovirus", 16, pp.545~555 (1996)
  9. "Early detection of human cytomegalovirus DNA by PCR-ELISA", 8, pp.407~413 (1998)
  10. "Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification", 158, pp.1177~84 (1988)
  11. "PCR amplification from paraffin-embedded tissues", 95, pp.117~24 (1991)
  12. "Quantitation of human cytomegalovirus DNA in leukocytes by end-point titration and duplex polymerase chain reaction", 49, pp.195~208 (1994)
  13. "Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection:Comparison with cell culture and shell vial assay", 32, pp.5~10
  14. "Cytomegalovirus antibody in normal persons and patients with chronic renal failure", 8, pp.901~6 (1988)
  15. "A survey of the cytomegalovirus antibodies in blood donors and the diseased", 1, pp.21~34 (1990)
  16. "Seroepidemiology of human cytomegalovirus in health adults measured by means of the anticomplement immunofluorescence technique", 24, pp.87~92 (1992)
  17. "Increased incidence of cytomegalovirus(CMV)infection and CMV-associated disease after allogenic bone marrow transplantation from unrelated donors", 19, pp.241~248 (1997)
  18. "Human cytomegalovirus structural components:intracellular and intraviral localization of p28 and p65-69 by immunoelectronic microscopy", 8, pp.15~23 (1987)
  19. "Prospective study of cytomegalovirus antigenemia in allograft recipients", 29, pp.1054~5 (1991)
  20. "Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromized patients", 30, pp.527~30 (1992)
  21. "Detection of cytomegalovirus in blood donor by the polymerase chain reaction", 33, pp.497~503 (1993)
  22. "Clinical use of a PCR test for detection of CMV DNA in leukocyte and serum samples", 26, pp.1717~ (1994)
  23. "Circulating immediate-early mRNA in patients with cytomegalovirus infections after solid organ transplantation", 170, pp.1264~7 (1994)
  24. "Comparison of LightCyler-based PCR, COBAS Amplicor CMV monitor, and pp65 antigenemia assays for quantitative measurement of cytomegalovirus viral load in peripheral blood specimens from patients after solid organ transplantation.", 41, pp.3167~3174 (2003)
  25. "Comparison of two quantitative CMV PCR test,Cobas Amplicor CMV monitor and TaqMan assay,and pp65-antigenemia assay in the determination of viral loads from peripheral blood of organ transplant patients", 30, pp.258~266 (2004)
  26. "Optimization of quantitative detection of cytomegalovirus DNA in plasma by real-time PCR", 42, pp.1142~1148 (2004)
  27. "Interference of heparin with the polymerase chain reaction", 9, pp.166~ (1990)
  28. "Regulation of cytomegalovirus late-gene expression:differential use of three start sites in the transcriptional activation of ICP36 gene expression", 63, pp.1783~91 (1989)
  29. "Immunoprecipitation of virus-specific immediate-early and early polypeptides from cells lytically infected with human cytomegalovirus strain AD 169", 112, pp.262~73 (1981)

관련 논문AI추천

항원혈증 검사법을 이용한 HCMV 질환의 신속한 진단 : pp65 항원혈증 검사의 중합효소연쇄반응 검사의 비교김의석, 김의종, 박완범, 신종욱, 오명돈, 이보빈, 최강원대한감염학회 · Infection and Chemotherapy2003 · 제35권 제1호인간 거대세포 바이러스 감염 진단을 위한 CMV 항원혈증 검사의 유용성 평가배재춘, 송운흥, 이남용, 이장호대한임상검사과학회 · 대한임상검사과학회지2000 · 제32권 제3호Comparison of Quantitation of Cytomegalovirus DNA by Real-Time PCR in Whole Blood with the Cytomegalovirus Antigenemia Assay고선영, 권선희, 이창규, 정보경, 조윤정대한진단검사의학회 · Annals of Laboratory Medicine2015 · 제35권 제1호거대세포바이러스 정량 검출을 위한 BiosewoomTM Real-Q CytomegalovirusQuantification kit 평가김명희, 김유리, 서장수, 오흥범, 원동일, 허운보대한진단검사의학회 · Annals of Laboratory Medicine2007 · 제27권 제4호Real-Time PCR과 일반 PCR을 이용한 Cytomegalovirus 정성검사의 비교 평가김문정, 김윤정, 배일권, 정석훈, 정세리대한임상미생물학회 · Annals of Clinical Microbiology2013 · 제16권 제1호신장이식 환자에서 HCMV 항원혈증 검사의 임상적 유용성김탁, 김성한, 김송철, 김양수, 박관태, 박수길, 성홍섭, 우준희, 이상오, 최상호, 한덕종대한감염학회 · Infection and Chemotherapy2009 · 제41권 제2호

이 간행물의 다른 논문 (12건)

균혈증 및 요로감염에서 분리된 장구균의 항균제 내성 및 병독성 인자 규명김선주, 박일중, 이혜수, 장철훈, 하경임, 김미나, 이경원, 허지영, 이위교, 김의종, 어영, 임인수pp. 77-83Multilocus Sequence Typing of Clonal Changes of Methicillin-resistant Staphylococcus aureus Isolated from Intensive Care Unit Patients: 1996 versus 2004홍기호, 김의종, 허지영, 이종윤pp. 84-89병원환경의 변화가 병원-획득성 요로감염에 미치는 영향박금보래, 이미경, 김혜련pp. 90-95CTX-M-12, PER-1 및 OXA-30 β-Lactamase 생성 Klebsiella pneumoniae의 출현이경원, 이유내, 배일권, 우건조, 곽효선, 최태열, 이위교, 용동은, 신종희, 김의종, 어영, 정석훈, 박연준, 홍성근, 안지영, 이성희, 이종욱pp. 102-109임상분리균주에 대한 은 나노입자(NANOVER)의 생체외 항균력 시험이원길, 이상흔, 허운보pp. 110-114
원문 보기