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거대세포바이러스 정량 검출을 위한 BiosewoomTM Real-Q CytomegalovirusQuantification kit 평가

Evaluation of BiosewoomTM Real-Q Cytomegalovirus Quantification kit for CytomegalovirusViral Load Measure

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참고문헌

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초록

배경 : 이식 수혜자에서 거대세포바이러스(cytomegalovirus,CMV) 감염에 대한 신속하고 정확한 검사가 필수적이다. 저자들은 최근 개발된 실시간 정량 PCR (RQ-PCR) 방법에 의한 CMV검사를 평가해 보고자 하였다.방법 : Real-Q Cytomegalovirus Quantification kit (Biose-woom Inc., Korea)를 사용한 CMV RQ-PCR의 분석 성능을평가하였고, 임상적 적용을 평가하기 위하여 이식 수혜자 63명을포함한 총 84명의 343검체를 대상으로 면역염색법인 p65 항원검사와 비교하였다. 결과 : RQ-PCR의 검출 한계는 63 copies/mL이었고, hepati-tis B virus (HBV), hepatitis C virus (HCV), human immu-nodeficiency virus (HIV) 양성 검체와 교차반응은 없었다. 정밀도는 총변이계수 10.4-19.5%이었으며, CMV DNA 1

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