저병원성 조류인플루엔자와 뉴캐슬 바이러스의 복합감염에 따른 닭 림프조직 병변의 특성 및 바이러스 검출
Pathology and virus distribution in the lymphoid tissues of chicks co-infection with H9N2 Avian influenza and Newcastle disease virus
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Low pathogenic avian influenza (LPAI; H9N2) and Newcastle disease (ND) are economically important poultry diseases in Korea. In this study, we investigated pathological features and virus distribution in the lymphoid tissues of chicks experimentally infected with H9N2 and/or ND virus. Six-weeks-old SPF chickens were divided into 4 groups, Control (C), H9N2 (E1), NDV (E2), and H9N2+NDV (E3). E1 group was challenged with 0.1 ml A/Kr/Ck/01310/01 (H9N2) 105.6 EID50 intranasally, E2 group was challenged with 0.5 ml KJW (NDV) 105.0∼106.0 ELD50 intramuscularly, and E3 group was challenged with H9N2, followed 7 days later by NDV. In histopathological examination, E1 group showed deple-tion and necrosis in bursa of Fabricius, thymus, cecal tonsil, and spleen, whereas E2 and E3 groups were noted severe lymphocyte depletion and necrosis with destruction of lymphoid organs structures. In TUNEL assay, apoptotic bodies were detected in lymphoid organs of all experimental groups, which was most severe in E3 group. H9N2 and ND viruses were predominantly detected in cecal tonsil of E1, E2, and E3 groups by PCR and immunohistochemistry (ICH). In conclusion, co-infection of H9N2 with NDV caused severe pathologic lesions and apoptosis in lymphoid tissues compared to single infections.
Abstract
Low pathogenic avian influenza (LPAI; H9N2) and Newcastle disease (ND) are economically important poultry diseases in Korea. In this study, we investigated pathological features and virus distribution in the lymphoid tissues of chicks experimentally infected with H9N2 and/or ND virus. Six-weeks-old SPF chickens were divided into 4 groups, Control (C), H9N2 (E1), NDV (E2), and H9N2+NDV (E3). E1 group was challenged with 0.1 ml A/Kr/Ck/01310/01 (H9N2) 105.6 EID50 intranasally, E2 group was challenged with 0.5 ml KJW (NDV) 105.0∼106.0 ELD50 intramuscularly, and E3 group was challenged with H9N2, followed 7 days later by NDV. In histopathological examination, E1 group showed deple-tion and necrosis in bursa of Fabricius, thymus, cecal tonsil, and spleen, whereas E2 and E3 groups were noted severe lymphocyte depletion and necrosis with destruction of lymphoid organs structures. In TUNEL assay, apoptotic bodies were detected in lymphoid organs of all experimental groups, which was most severe in E3 group. H9N2 and ND viruses were predominantly detected in cecal tonsil of E1, E2, and E3 groups by PCR and immunohistochemistry (ICH). In conclusion, co-infection of H9N2 with NDV caused severe pathologic lesions and apoptosis in lymphoid tissues compared to single infections.
참고문헌 (30)
- 김기석, "조류질병학", pp.3~9 (2012), 도서출판 한미의학
- 모인필, "Avian influenza의 병성기전", Annual Rev Anim Immunol Res Inst, (2), pp.67~70 (1993)
- 하봉도, "저병원성 조류인플루엔자(H9N2)에 대한 사독 오일백신의 안전성 및 효능 평가", 박사, 수의임상학, pp.2007
- 한명국, "가금인플루엔자 바이러스 항체검출을 위한 혈청학적 진단법 비교", 대한수의학회지, 42(1), pp.9~80 (2002), 대한수의학회
- Alexander DJ, "Diseases of poultry", pp.75~100 (2008), Blackwell Publishing
- Alexander DJ, "A review of avian influenza in different bird species", Vet Microbiol, 74, pp.3~13 (2000)
- Brown CC, "Comparison of pathologybased techniques for detection of viscerotropic velogenic Newcastle disease virus in chickens", J Comp Pathol, 120, pp.383~389 (1999)
- Capua I, "Control of Avian Influenza in poultry", Emerg Infect Dis, 12, pp.1319~1324 (2006)
- Cattoli G, "Newcastle disease : a review of field recognition and current methods of laboratory detection", J Vet Diagn Invest, 23, pp.637~656 (2011)
- Fang J, "Developmental changes in cell proliferation and apoptosis in the normal duck thymus", Anat Histol Embryol, 40, pp.457~465 (2011)
- Franca MS, "Influenza pathobiology and pathogenesis in avian species", Curr Top Microbiol Immunol, 385, pp.221~242 (2014)
- Holt PS, "Changes in Peyer’s patch and cecal tonsil B lymphocytes in laying hens following challenge with Salmonella enterica serovar enteritidis", Int J Poult Sci, 10, pp.231~237 (2011)
- Hooper PT, "Immunohistochemistry in the identification of a number of new diseases in Australia", Vet Microbiol, 68, pp.89~93 (1999)
- 김재홍, "Characterization and comparison of the pathogenicity of viscerotropic velogenic Newcastle disease virus isolates in Korea", 대한수의학회지, 52(4), pp.213~221 (2012), 대한수의학회
- Kwon JS, "Immune responses and pathogenesis in immunocompromised chickens in response to infection with the H9N2 low pathogenic avian influenza virus", Virus Res, 133, pp.187~194 (2008)
- Lam KM, "Newcastle disease virus-induced apoptosis in the peripheral blood mononuclear cells of chickens", J Comp Pathol, 114, pp.63~71 (1996)
- 이성민, "닭에서 저병원성 조류인플루엔자와 뉴캐슬 바이러스의 복합감염에 따른 임상적, 병리학적 연구", 한국동물위생학회지, 36(3), pp.163~169 (2013), 한국동물위생학회
- Lindskog C, "European H16N3 gull influenza virus attaches to the human respiratory tract and eye", PLoS One, 8, pp.e60757~ (2013)
- Lowy RJ, "Influenza virus induction of apoptosis by intrinsic and extrinsic mechanisms", Int Rev Immunol, 22, pp.425~449 (2003)
- Playford EG, "Laboratory diagnosis of influenza virus infection", Pathology, 34, pp.115~125 (2002)
- Rautenschlein S, "Pathogenic avian adenovirus type II induces apoptosis in turkey spleen cells", Arch Virol, 145, pp.1671~1683 (2000)
- Rezaian M, "Histological study of the caecal tonsil in the cecum of 4-6 months of age White Leghorn Chicks", Am J Anim Vet Sci, 2, pp.50~54 (2007)
- Seal BS, "The avian response to Newcastle disease virus", Dev Comp Immunol, 24, pp.257~268 (2000)
- Sinkovics JG, "Newcastle disease virus(NDV) : brief history of its oncolytic strains", J Clin Virol, 16, pp.1~15 (2000)
- Spackman E, "The pathogenesis of low pathogenicity H7 avian influenza viruses in chickens, ducks and turkeys", Virol J, 7, pp.331~ (2010)
- Suarez DL., "Immunology of avian influenza virus : a review", Dev Comp Immunol, 24, pp.269~283 (2000)
- Susta L, "Clinicopathological characterization in poultry of three strains of Newcastle disease virus isolated from recent outbreaks", Vet Pathol, 48, pp.349~360 (2011)
- Swayne DE, "Diseases of poultry", pp.153~184 (2008), Blackwell Publishing
- Xing Z, "Differential regulation of antiviral and proinflammatory cytokines and suppressionof Fas-mediated apoptosis by NS1 of H9N2 avian influenza virus in chicken macrophages", J Gen Virol, 90, pp.1109~1118 (2009)
- Zaid A, "Apoptosis in lymphoid organs in chicken and duck : a comparative study", Alexandria J Vet Sci, 49, pp.95~102 (2016)