닭에서 저병원성 조류인플루엔자와 뉴캐슬 바이러스의 복합감염에 따른 임상적, 병리학적 연구
Clinical and pathological studies on co-infection of lowpathogenic avian influenza virus and Newcastle disease virus in the chicken
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Both of avian influenza (AI) and Newcastle disease (ND) can cause mild to severe diease in poultry. In this study, clinical signs, macro, and micro lesions were studied. Eighteen six-week-old SPF chicks were divided into 4 groups (E1, E2, E3 and C1) and housed in different rooms of the isolation facility at CAVAC (Daejeon, Korea). The control group (C1) of 3 chicks was housed separately as uninoculat-ed. Experimental groups (E1, E2 and E3) challenged with H9N2 and/or NDV. E1 group was challenged with 0.1 mL A/Kr/Ck/01310/01 (H9N2) 105.6 EID50 by intranasal, E2 group was challenged with 0.5 mL Kyojeongwon (KJW) 105.0~106.0 ELD50 by intramuscular, and E3 group was challenged with 0.1 mL A/Kr/Ck/01310/01 105.6 EID50 by intranasal and 0.5 mL KJW 105.0~106.0 ELD50 by intramuscular 7 days after H9N2 challenge. In clinical signs and gross findings, E1 group showed 0% mortality, ano-rexia, and hemorrhage of proventriculus and thymus, E2 group showed 100% mortality within 3∼5 days after challenge, anorexia, green diarrhea, hemorrhage of proventriculus, proximal esophagus and thymus, enlargement of kidney, and bronze liver, and E3 group showed 100% mortality within 24∼36 hours after NDV challenge, depression, anorexia, green diarrhea, hemorrhage of proventriculus, spleen, and lung, enlargement of kidney, and reduction of thymus size and number. In histopathological exami-nation, E1 group showed depletion and necrosis in bursa of Fabricius, thymus, and spleen, and E2 and E3 group showed severe lymphocyte depletion and necrosis with destruction of lymphoid organ structures. In conclusion, co-infection of H9N2 with ND virus causes acute disease with high mortality than single infection and the pathologic lesions were more severe.
Abstract
Both of avian influenza (AI) and Newcastle disease (ND) can cause mild to severe diease in poultry. In this study, clinical signs, macro, and micro lesions were studied. Eighteen six-week-old SPF chicks were divided into 4 groups (E1, E2, E3 and C1) and housed in different rooms of the isolation facility at CAVAC (Daejeon, Korea). The control group (C1) of 3 chicks was housed separately as uninoculat-ed. Experimental groups (E1, E2 and E3) challenged with H9N2 and/or NDV. E1 group was challenged with 0.1 mL A/Kr/Ck/01310/01 (H9N2) 105.6 EID50 by intranasal, E2 group was challenged with 0.5 mL Kyojeongwon (KJW) 105.0~106.0 ELD50 by intramuscular, and E3 group was challenged with 0.1 mL A/Kr/Ck/01310/01 105.6 EID50 by intranasal and 0.5 mL KJW 105.0~106.0 ELD50 by intramuscular 7 days after H9N2 challenge. In clinical signs and gross findings, E1 group showed 0% mortality, ano-rexia, and hemorrhage of proventriculus and thymus, E2 group showed 100% mortality within 3∼5 days after challenge, anorexia, green diarrhea, hemorrhage of proventriculus, proximal esophagus and thymus, enlargement of kidney, and bronze liver, and E3 group showed 100% mortality within 24∼36 hours after NDV challenge, depression, anorexia, green diarrhea, hemorrhage of proventriculus, spleen, and lung, enlargement of kidney, and reduction of thymus size and number. In histopathological exami-nation, E1 group showed depletion and necrosis in bursa of Fabricius, thymus, and spleen, and E2 and E3 group showed severe lymphocyte depletion and necrosis with destruction of lymphoid organ structures. In conclusion, co-infection of H9N2 with ND virus causes acute disease with high mortality than single infection and the pathologic lesions were more severe.
참고문헌 (23)
- 김기석, "뉴캣슬병, 조류인플루엔자, In 조류질병학. 초판", pp.3~39 (2012), 도서출판한미의학
- 모인필, "Avian influenza의 병성기전", Annual Rev Ani Immunol Res Inst, (2), pp.67~70 (1993)
- Alexander DJ, "Newcastle disease and other avian Paramyxoviridae infections, In Diseases of Poultry. 10th ed", pp.541~569 (1997), Iowa State University Press
- Alexander DJ, "A review of avian influenza in different bird species", Vet Microbiol, 74, pp.3~13 (2000)
- Alexander DJ, "Newcastle disease and other avian paramyxoviruses", Rev Sci Tech, 19, pp.443~462 (2000)
- Barbour EK, "Evaluation of the Histopathology of the respiratory system in essential oil-treated broilers following a challenge with Mycoplasma gallisepticum and", or H9N2 Influenza virus, 2006, pp.4, 293~300
- Brown CC, "Comparison of pathology- based techniques for detection of viscerotropic velogenic Newcastle disease virus in chickens", J Comp Pathol, 120, pp.383~389 (1999)
- Capua I, "Prevention and control of highly pathogenic avian influenza with particular reference to H5N1", Virus Res (2013)
- Cattoli G, "Newcastle disease: a review of field recognition and current methods of laboratory detection", J Vet Diagn Invest, 23, pp.637~656 (2011)
- Falcon MD, "Exotic Newcastle Disease", Semin Avian and Exotic Pet Med, 13, pp.79~85 (2004)
- 한명국, "가금인플루엔자 바이러스 항체검출을 위한 혈청학적 진단법 비교", 대한수의학회지, 42(1), pp.9~80 (2002), 대한수의학회
- Yu-Ping Hua, "Primary survey of avian influenza virus and Newcastle disease virus infection in wild birds in some areas of Heilongjiang Province,China", Journal of Veterinary Science, 6(4), pp.311~315 (2005), 대한수의학회
- 김재홍, "Characterization and comparison of the pathogenicity of viscerotropic velogenic Newcastle disease virus isolates in Korea", 대한수의학회지, 52(4), pp.213~221 (2012), 대한수의학회
- Kwon JS, "Immune responses and pathogenesis in immunocompromised chickens in response to infection with the H9N2 low pathogenic avian influenza virus", Virus Res, 133, pp.187~194 (2008)
- 이진성, "약병원성 조류인플루엔자 사독백신개발을 위한 후보주(A0401)의 면역 원성 및 안정성 평가", 한국가금학회지, 32(2), pp.113~124 (2005), 한국가금학회
- Naeem K, "Seroprevalence of avian influenza virus and its relationship with increased mortality and decreased egg production", Avian Pathol, 32, pp.285~289 (2003)
- Nili H, "Natural cases and an experimental study of H9N2 avian influenza in commercial broiler chickens of Iran", Avian Pathol, 31, pp.247~252 (2002)
- Oldoni I, "The use of in situ hybridization and immunohistochemistry to study the pathogenesis of various Newcastle disease virus strains and recombinants in embryonated chicken eggs", Microb Pathog, 39, pp.69~75 (2005)
- Park KS, "Epizootiological study on the outbreaks of Newcastle disease in Korea", Korean J Poult Sci, 6, pp.38~46 (1979)
- Rasamoelina Andriamanivo H, "Risk factors for avian influenza and Newcastle disease in small holder farming systems. Madagascar highlands", Prev Vet Med, 104, pp.114~124 (2012)
- Seal BS, "Characterization of Newcastle disease virus vaccines by biological properties and sequence analysis of the hemagglutinin-neuraminidase protein gene", Vaccine, 14, pp.761~766 (1996)
- Sinkovics JG, "Newcastle disease virus (NDV): brief history of its oncolytic strains", J Clin Virol, 16, pp.1~15 (2000)
- Vervelde L, de, "Contribution of the genetic background to the immune response of broilers vaccinated or challenged with LPAI H9N2", BMC Proc, 5(Suppl 4), pp.S5~ (2011)