본문 바로가기
KERD
논문

층층잔대 수집자원의 형태적 특성과 SSR마커를 이용한 유전다양성 분석

Investigation of Morphological Characteristics and Genetic Diversity of Adenophora triphylla (Thunb.) A. DC. Using SSR Markers

김지아|정대희|엄유리|윤영배|허정훈|박홍우
한국약용작물학회|2022.12|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제30권 제6호|pp. 411-418

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

Adenophora triphylla (Thunb.) A. DC.Genetic DiversityMorphological Characteris￾ticsSimple Sequence Repeats

초록

Background: Adenophora triphylla (Thunb.) A. DC. is used for food and medicinal purposes in Eastern Asia including the Republic of Korea. Methods and Results: There have been no studies on the genetic diversity of A. triphylla. The aim of this study was to investigate the genetic diversity of A. triphylla resources using simple sequence repeat (SSR) markers and to provide basic data for the molecular breeding of A. triphylla. Using eight SSR markers, the genetic diversity of 40 A. triphylla resources was found to be different in various cultivation areas. Genetic analysis using the eight SSR markers revealed a high level of diversity among the genetic resources. In the unweighted pair group method with arithmetic mean cluster analysis, the 40 A. triphylla genetic resources were divided into clade A and clade B. In clade A, the external morphological characteristics (stem color, leaf shape, and flower color) were identical; however, in clade B, a mixed pattern was observed. Conclusions: These results may be used as basic data for molecular breeding to identify origin of plants and analyze their relationships using SSR markers.

Abstract

Background: Adenophora triphylla (Thunb.) A. DC. is used for food and medicinal purposes in Eastern Asia including the Republic of Korea. Methods and Results: There have been no studies on the genetic diversity of A. triphylla. The aim of this study was to investigate the genetic diversity of A. triphylla resources using simple sequence repeat (SSR) markers and to provide basic data for the molecular breeding of A. triphylla. Using eight SSR markers, the genetic diversity of 40 A. triphylla resources was found to be different in various cultivation areas. Genetic analysis using the eight SSR markers revealed a high level of diversity among the genetic resources. In the unweighted pair group method with arithmetic mean cluster analysis, the 40 A. triphylla genetic resources were divided into clade A and clade B. In clade A, the external morphological characteristics (stem color, leaf shape, and flower color) were identical; however, in clade B, a mixed pattern was observed. Conclusions: These results may be used as basic data for molecular breeding to identify origin of plants and analyze their relationships using SSR markers.

참고문헌 (29)

  1. 방경환, "SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석", 한국약용작물학회지, 19(5), pp.347~353 (2011), 한국약용작물학회
  2. Cavagnaro PF, "Genome-wide characterization of simple sequence repeats in cucumber(Cucumis sativus L. )", BMC Genomics, 11, pp.569~586 (2010)
  3. Cheng Y, "Development of simple sequence repeat(SSR)markers to study diversity in the herbaceous peony(Paeonia lactiflora)", Journal of Medicinal Plants Research, 5, pp.6744~6751 (2011)
  4. Chinese Pharmacopoeia Commission(CPC), , pp.96~107 (2010), Pharmacopoeia of the people’s Republic of China 2010 edition
  5. 최고야, "동북아 5개국 공정서의 식물성 한약재 기원종 비교 : 同名異屬種을 중심으로", 대한본초학회지, 28(5), pp.103~111 (2013), 대한본초학회
  6. 함영안, "잔대 추출물들의 항돌연변이 및 항종양 효과", 한국식품영양과학회지, 38(1), pp.25~31 (2009), 한국식품영양과학회
  7. Japanese Ministry of Health, Labour and Welfare(JMHLW)., "The Japanese Pharmacopoeia 16th edition. The Ministry of Health, Labour and Welfare Ministerial Notification No. 65", Japanese Ministry of Health, 162~170 (2011)
  8. 정대희, "Genome-Wide SSR 마커를 이용한 주요 산지별 참당귀의 유전다양성 분석", 한국약용작물학회지, 27(6), pp.376~382 (2019), 한국약용작물학회[DOI]
  9. Jin L, "Estimation of genetic distance and coefficient of gene diversity from single-probe multilocus DNA fingerprinting data", Molecular Biology and Evolution, 11, pp.120~127 (1994)
  10. 조익현, "미토콘드리아 DNA 염기서열 변이를 이용한 인삼 종 판별 연구", 한국약용작물학회지, 21(2), pp.91~96 (2013), 한국약용작물학회[DOI]
  11. Khaing AA, "Phylogenetic relationships of chrysanthemums in Korea based on novel SSR markers", Genetics and Molecular Research, 12, pp.5335~5347 (2013)
  12. Kim HJ, "The chemotaxonomic classification of Korean Cam-panulaceae based on triterpene, sterol, and polyacetylene contents", Biochemical Systematics and Ecology, 74, pp.11~18 (2017)
  13. 김정훈, "한국산 둥굴레속 식물의 형태적 특성 및 엽록체 DNA 염기서열을 이용한 유연관계 분석", 한국약용작물학회지, 22(6), pp.489~496 (2014), 한국약용작물학회[DOI]
  14. 김경아, "엽록체 DNA 염기서열 분석을 이용한 한국산 초롱꽃과(Campanulaceae)의 계통유연관계", 식물분류학회지, 42(4), pp.282~293 (2012), 한국식물분류학회
  15. 김세림, "Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis", Journal of Plant Biotechnology, 43(2), pp.181~188 (2016), 한국식물생명공학회[DOI]
  16. Korea Food & Drug Administration(KFDA)., "The Korean herbal pharmacopoeia. The KFDA Notification No. 2012-135", Korea Food and Drug Administration, 67~83 (2012)
  17. Lee JK, "A taxonomic study of the genus Adenophora in Korea. Sung Kyun Kwan University", Journal of nature science, 45, pp.15~34 (1994)
  18. Li Gang, "Genetic Diversity Analysis of Mungbean Accessions from East and Central Asia Using SSR Markers", 한국국제농업개발학회지, 23(2), pp.185~193 (2011), 한국국제농업개발학회
  19. Liu K, "PowerMarker : An integrated analysis environment for genetic marker analysis", Bioinformatics, 21, pp.2128~2129 (2005)
  20. 문병철, "AFLP 분석을 통한 Curcuma속 수집종간 유전적 다양성 분석", 한국약용작물학회지, 21(6), pp.455~460 (2013), 한국약용작물학회[DOI]
  21. Oh SY, "A review of the family Campanulaceae of Korea", Research Review of Kyungpook National University, 31, pp.311~386 (1981)
  22. 박기찬, "차세대염기서열분석법을 이용한 잔대의 SSR 마커 개발", 한국약용작물학회지, 25(6), pp.411~417 (2017), 한국약용작물학회[DOI]
  23. 박상익, "당귀 종판별을 위한 엽록체 기반 SSR 마커 개발", 한국약용작물학회지, 24(4), pp.317~322 (2016), 한국약용작물학회[DOI]
  24. Peng J, "Genetic diversity and population structure of the medicinal plant Docynia delavayi(Franch. )Schneid revealed by transcriptome-based SSR markers", Plants Journal of Applied Research on Medicinal and Aromatic Plants, 21, pp.100294~ (2021)
  25. Song JY, "Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorum(Campanulaceae)", Genetics and Molecular Research, 11, pp.3263~3266 (2012)
  26. 엄유리, "Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers", Plant Breeding and Biotechnology, 4(1), pp.71~78 (2016), 한국육종학회[DOI]
  27. Yang YJ., "Anti-inflammatory effects of natural Adenophora triphylla var. japonica Hara water extracts. Department of Alternative Medicine, Master Degree Thesis, Chosun University, Korea. p", pp.2008
  28. Yoo KO., "Taxonomic studies on the Korean Campanulaceae. Department of Biology, Ph. D Thesis, Kangwon National University, Korea. p", pp.1995
  29. Zhu H, "Mining and validation of pyrosequenced simple sequence repeats(SSRs)from American cranberry(Vaccinium macrocarpon Ait. )", Theoretical and Applied Genetics, 124, pp.87~96 (2011)

관련 논문AI추천

도라지 수집종의 형태적 특성과 SSR마커에 의한 유연관계 분석이이, 김금숙, 김옥태, 김창국, 엄유리, 이대영, 이재원, 홍창표, Mei Lan Jin한국약용작물학회 · 한국약용작물학회지2016 · 제24권 제1호차세대염기서열분석법을 이용한 잔대의 SSR 마커 개발이이, 정희, 길진수, 김영국, 박기찬, 박신기, 홍창표, 황보경한국약용작물학회 · 한국약용작물학회지2017 · 제25권 제6호Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis이이, 정희, 길진수, 김세림, 김옥태, 김용열, 김지현, 김태동, 김호방, 엄유리, 유제민, 이동훈, 정지희한국식물생명공학회 · Journal of Plant Biotechnology2016 · 제43권 제2호Genetic diversity assessment of lily genotypes native to Korea based on simple sequence repeat markers김영선, 이긍주, 장지영, Bashistha Kumar Kanth, Shipra Kumari한국식물생명공학회 · Journal of Plant Biotechnology2019 · 제46권 제3호Genome-Wide SSR 마커를 이용한 주요 산지별 참당귀의 유전다양성 분석이이, 길진수, 김기윤, 김만조, 박윤미, 박홍우, 엄유리, 전권석, 정대희한국약용작물학회 · 한국약용작물학회지2019 · 제27권 제6호한국특산종 외대잔대(Adenophora racemosa)의 분자계통학적 위치김호경, 문병철, 이아영, 지윤의, 천진미, 추병길한국약용작물학회 · 한국약용작물학회지2010 · 제18권 제6호

이 간행물의 다른 논문 (7건)

Protective Effect of Polysaccharide Derived from Natural Extract on Human Follicle Dermal Papilla Cell and Keratinocytes방원정, 김지영, 이창석, 이성현, 주영협pp. 391-400Comparison of Major Compounds of Coffee Tree Leaf Extract by Varieties Cultivated in Korea백지환, 이경인, 표병식, 차상화pp. 401-410괭이밥 열수 추출물의 항산화 및 항염증 효과홍 민, Hoang Viet Bach Khoa, Luong Van Dung, 안용조, 이현주, 최다혜, 권태형, 한준희, 김종희pp. 419-429모란과 작약의 구별을 위한 엽록체 기반 InDel 마커의 개발윤기훈, 박소현, 정희정, 이재복, 김세림, 정진태, 김문교, 이이, 길진수, 이미선, 정 희pp. 430-439인삼 재배 시 양분수준이 침수피해에 미치는 영향박준홍, 유진, 김영창, 구성철, 장인복, 서수정pp. 440-449
원문 보기