Bacillus spp.에 의한 황기의 생육촉진 및 토양병해 방제효과
Effect of Bacillus spp. on Growth Promotion and Control of Soil Borne Diseases of Astragalus membranaceus Bunge
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Background: This study investigated potential biological control activities, involving the promotion of growth and antifungal activity against Fusarium oxysporum and Phytophthora drechsleri. Methods and Results: Among the microorganisms isolated from an Astragalus cultivation area and from Korean kimchi, three species were selected. These included A11, which displayed excellent pathogen mycelial suppression ability in dual culture tests. It was identified as Bacillus sp. on the basis of 16s rRNA sequencing. Growth promoting ability was tested by dispensing the three selected strains in pots, to be cultured for 4 weeks. The strain A11 increased the fresh plant weight by 50.8% and increased the fresh root weight by 36.6% compared to untreated samples. The respective increases of the other strain were 18.4% and 35.6% by strain, A10, and 28.0% and 31.1% by strain B2-087. In terms of pathogen control, when the A11, A10, and B2-087 were alternatively tested for 4 weeks against F. oxysporum or P. drechsleri, the A11 control rate was 74.1% compared to the group that received no treatment. Meanwhile, the others had shown control rates between 70.4% and 18.5%. Conclusions: The three selected strains induced the growth of A. membranaceus Bunge, which suppressed the development of root rot and wilt disease.
Abstract
Background: This study investigated potential biological control activities, involving the promotion of growth and antifungal activity against Fusarium oxysporum and Phytophthora drechsleri. Methods and Results: Among the microorganisms isolated from an Astragalus cultivation area and from Korean kimchi, three species were selected. These included A11, which displayed excellent pathogen mycelial suppression ability in dual culture tests. It was identified as Bacillus sp. on the basis of 16s rRNA sequencing. Growth promoting ability was tested by dispensing the three selected strains in pots, to be cultured for 4 weeks. The strain A11 increased the fresh plant weight by 50.8% and increased the fresh root weight by 36.6% compared to untreated samples. The respective increases of the other strain were 18.4% and 35.6% by strain, A10, and 28.0% and 31.1% by strain B2-087. In terms of pathogen control, when the A11, A10, and B2-087 were alternatively tested for 4 weeks against F. oxysporum or P. drechsleri, the A11 control rate was 74.1% compared to the group that received no treatment. Meanwhile, the others had shown control rates between 70.4% and 18.5%. Conclusions: The three selected strains induced the growth of A. membranaceus Bunge, which suppressed the development of root rot and wilt disease.
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