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인삼 점무늬병균(Alternaria panax)과 탄저병균(Colletotrichum gloeosporioides)에 대한 길항미생물 Bacillus spp. 선발

Screening of Antifungal Bacillus spp. against Alternaria Blight Pathogen (Alternaria panax) and Anthracnose Pathogen (Colletotrichum gloeosporioides) of Ginseng

이승호|박기춘|강승원|박홍우|방경환|현동윤|정일민|차선우|이혜진
한국약용작물학회|2012.10|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제20권 제5호|pp. 339-344

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참고문헌

키워드

GinsengColletotrichum gloeosporioidesBacillusBiological ControlAlternaria panaxAntagonistic Microorganism

초록

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

Abstract

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

참고문헌 (27)

  1. Bae YS, "Handbook of ginseng diseases and pests", National Institute of Crop Science Press, 1~79 (2005)
  2. Baker CJ, "Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on leaves development of rust pustules on bean leaves", Journal of Phytopathology, 73, pp.1148~1152 (1983)
  3. 조정일, "참다래 꼭지썩음병을 일으키는 Diaporthe actinidiae을 억제하는 길항성 Bacillus sp. #72의 분리 및 동정", 한국지역사회생활과학회지, 18(2), pp.241~246 (2007), 한국지역사회생활과학회
  4. 정기채, "인삼 주요병에 대한 길항미생물 선발", 한국약용작물학회지, 14(4), pp.202~205 (2006), 한국약용작물학회
  5. Gillis CN, "Panax ginseng pharmacology: A nitiric oxide link", Biochemical Pharmacology, 54, pp.1~8 (1997)
  6. Glick BR, "Promotion of plant growth by ACC deaminase-producing soil bacteria", European Journal of Plant Pathology, 119, pp.329~339 (2007)
  7. Han OK, "Chitinase of multifunctional antagonistic bacterium Bacillus amyloliquefaciens 7079 against phytopathogenic fungi", Korean Journal of Microbiology and Biotechnology, 29, pp.142~148 (2001)
  8. Hu SY, "The genus panax(Ginseng) in chinese medicine", Economic Botany, 30, pp.11~28 (1976)
  9. 정우철, "잔디 갈색퍼짐병(Large patch)의 생물학적 방제를 위한 길항미생물의 선발과 효력 검정", 식물병 연구, 14(1), pp.43~50 (2008), 한국식물병리학회
  10. Kim YH, "Effect of shading on the quality of raw, red and white ginseng and the contents of some minerals in ginseng roots", Journal of Ginseng Research, 14, pp.36~43 (1990)
  11. 김영숙, "인삼뿌리썩음병 방제에 유효한 길항미생물의 탐색", 한국균학회지, 40(1), pp.44~48 (2012), 한국균학회
  12. 이병대, "Bacillus subtilis B-4228의 인삼 근부병 억제효과", Journal of Ginseng Research, 28(1), pp.67~70 (2004), 고려인삼학회
  13. 이건웅, "Bacillus subtilis S54 균주를 이용한 고추 역병과 탄저병의 생물학적 방제", 식물병 연구, 17(1), pp.86~89 (2011), 한국식물병리학회
  14. Lee JH, "Morphological characteristics and pathogenicity of Alternaria isolates causing leaf and stem blight and black root rot of Korea ginseng", Plant Pathology Journal, 6, pp.13~20 (1990)
  15. 이성우, "석회보르도액 처리가 3년생 인삼의 생육과 진세노사이드 및 엑스 함량에 미치는 영향", 한국약용작물학회지, 18(4), pp.244~247 (2010), 한국약용작물학회
  16. Lee WH, "Advantages and disadvantage in using biological control of plant diseases and integrated control", Journal of Agriculture & Life Science, 39, pp.66~76 (2008)
  17. Lim SY, "Isolation and numerical identification of Streptomyces humidus strain S5-55 antagonistic to plant pathogenic fungi", Plant Pathology Journal, 16, pp.189~199 (2000)
  18. 남기열, "고려인삼의 방사선 방어효과에 대한 연구현황과 전망", 한국약용작물학회지, 19(4), pp.287~299 (2011), 한국약용작물학회
  19. Nei M, "Molecular evolution and phylogenetics", pp.1~333 (2000), Oxford University Press
  20. Mehnaz S, "Isolation and 16S rRNA sequence analysis of the beneficial bacteria from the rhizosphere of rice", Canadian Journal of Microbiology, 47, pp.110~117 (2001)
  21. Msadek T, "ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation", Molecular Microbiology, 27, pp.899~914 (1998)
  22. 장문석, "人蔘이 수컷 생쥐의 정자 수와 CatSper3, 4 단백질 발현에 미치는 영향", 대한본초학회지, 27(2), pp.47~51 (2012), 대한본초학회
  23. Reysenbach AL, "Differential amplification of rDNA genes by polymerase chain reaction", Applied and Environmental Microbiology, 58, pp.3417~3418 (1992)
  24. Saitou N, "The neighbor-joining method: anew method for reconstructing phylogenetic trees", Journal of Molecular Biology, 4, pp.406~425 (1987)
  25. Tamura K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version4.0", Molecular Biology and Evolution, 24, pp.1596~1599 (2007)
  26. 우수영, "Growth and Eco-Physiological Characteristics of Panax ginseng Grown under Three Different Forest Types", Journal of Plant Biology(한국식물학회지), 47(3), pp.230~235 (2004), 한국식물학회
  27. 용영록, "유용길항균이 배추 뿌리혹병 방제에 미치는 영향", 원예과학기술지, 21(3), pp.194~198 (2003), 한국원예학회

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