고추역병 유발병원균 Phytophthora capsici에 대한 Bacillus sp. AM-651의 항진균활성
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초록
Biological antagonists of Phytophthora capsici were isolated from soil in Gyeongbuk, Korea. Among the isolated bacteria, a Bacillus sp. was identified from 16S rDNA sequence analysis and named Bacillus sp. AM-651. Bacillus sp. AM-651 strain which can strongly a antifungal activity against Phytophthora capsici. Culture conditions for the maximum production of the antagonistic substance were optimized. The production of antibiotic were high on modified Davis mineral medium pH 7 at 30oC. The medium for highest production of the agonistic substance optimized. It is composed the best activity on glucose, (NH4)2SO4 and K2HPO4 at 0.5%, 0.1%, and 0.7%, respectively. By time course of culture solution selected Bacillus sp. AM-651, the culture solution after 48hrs had strongly growth inhibition rate against P.capsici. And culture solution of Bacillus sp. AM-651 was stable within a pH range 5~11 and temperature range 4~70oC. Bacillus sp. AM-651 cultured broth shown fungal growth inhibitory activitiy against B. sorokiniana, B. cinerea, R. solani avove and beyond P. capsici and comparatively showed a high activity against C. gloeosporioides, B. dothidea, B. cinerea and F. graminearum by agar diffusion method
Abstract
Biological antagonists of Phytophthora capsici were isolated from soil in Gyeongbuk, Korea. Among the isolated bacteria, a Bacillus sp. was identified from 16S rDNA sequence analysis and named Bacillus sp. AM-651. Bacillus sp. AM-651 strain which can strongly a antifungal activity against Phytophthora capsici. Culture conditions for the maximum production of the antagonistic substance were optimized. The production of antibiotic were high on modified Davis mineral medium pH 7 at 30oC. The medium for highest production of the agonistic substance optimized. It is composed the best activity on glucose, (NH4)2SO4 and K2HPO4 at 0.5%, 0.1%, and 0.7%, respectively. By time course of culture solution selected Bacillus sp. AM-651, the culture solution after 48hrs had strongly growth inhibition rate against P.capsici. And culture solution of Bacillus sp. AM-651 was stable within a pH range 5~11 and temperature range 4~70oC. Bacillus sp. AM-651 cultured broth shown fungal growth inhibitory activitiy against B. sorokiniana, B. cinerea, R. solani avove and beyond P. capsici and comparatively showed a high activity against C. gloeosporioides, B. dothidea, B. cinerea and F. graminearum by agar diffusion method
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