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근권 토양과 뿌리로부터 분리된 Bacillus sp.의 항진균 활성, 식물 생장 촉진 활성 및 미네랄 가용화능 비교

Comparison of the Antifungal Activity, Plant Growth Promoting Activity, and Mineral Solubilization Ability of Bacillus sp. Isolated from Rhizosphere Soils and Roots

이상현|이광희|장정수|이송민|Ji-Youn Kim|김희숙|오가윤
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2021.12|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제49권 제4호|pp. 576-586|DOI 10.48022/mbl.2106.06003

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키워드

Bacillus sp.extracellular enzymeplant growth promoting rhizobacteriamicrobial agentmineral solubilization
주제분류생물학

초록

본 연구는 근권 토양 및 뿌리에 존재하는 미생물 중 미생물 제제로 적합한 Bacillus sp.을 선별하기 위하여 10종의 식물 병원성 곰팡이에 대한 항진균 활성, 식물 생장 촉진 활성및 미네랄 가용화능을 평가하였다. 식물 병원성 곰팡이에 대한 항진균 활성은 Bacillus sp.에 따른 차이를 보였으며, 선별된 균주 중 DDP4, DDP16, DDP148, SN56, SN95는 9종이상의 식물 병원성 곰팡이에 대해 항진균 활성을 나타내었다. 질소고정능과 1-aminocyclopropane-1-carboxylic acid deaminase 생성능에서는 Bacillus sp. 모두 비슷한 수준으로 활성을 나타냈으며, siderophore 생성능은 ANG42와DDP427에서 비교적 높게 나타났다. Indole-3-acetic acid 생성능은 1.83−67.91 μg/ml으로 확인되었으며, Bacillus sp.에따른 활성 차이를 나타내었다. 이후 각 species마다 활성이높은 1 균주씩 선정하여 미네랄 가용화능을 확인한 결과, 대부분의 Bacillus sp.에서 인산과 탄산칼슘 가용화능을 확인할 수 있었으며, DDP148과 SN56은 각각 규소 가용화능과아연 가용화능도 나타내었다. 따라서, 본 연구에서 분리한Bacillus sp.의 항진균 활성, 식물 생장 촉진 활성 및 미네랄가용화능 결과를 통해 다목적 기능을 가진 미생물 제제로써활용이 가능할 것으로 판단된다.

Abstract

The purpose of this study was to evaluate the antifungal activity, plant-growth-promoting activity, and mineral solubilization ability of 10 species of phytopathogenic fungi to select a Bacillus sp. from rhizosphere soils and roots that can be used as a microbial agent. The antifungal activity for phytopathogenic fungi varied based on the Bacillus sp. Among the selected strains, DDP4, DDP16, DDP148, SN56, and SN95 exhibited antifungal activity for nine or more species of phytopathogenic fungi. Regarding nitrogen-fixation ability, all Bacillus sp. showed similar levels of activity, and siderophore production ability was relatively high in ANG42 and DDP427. The indole-3-acetic acid production abilities were in the range of 1.83-67.91 μg/ ml, with variations in activity based on the Bacillus sp. One strain with a high activity was selected from each species, and their mineral solubilization abilities were examined. Most Bacillus sp. could solubilize phosphoric acid and calcium carbonate, and DDP148 and SN56 could solubilize silicon and zinc, respectively. These results suggested that Bacillus sp. can be considered potential multi-purpose microbial agents for plant growth promotion and disease prevention.

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근권에 존재하는 Bacillus 속 균주들의 식물 생장 촉진 활성 특성김지윤, 김희숙, 오가윤, 이광희, 이상현, 이송민, 장정수한국미생물·생명공학회 · 한국미생물·생명공학회지2021 · 제49권 제3호식물 병원성 곰팡이에 길항작용을 갖는 다양한 Bacillus sp.의 균주 분리와 특성에 관한 연구김지윤, 김희숙, 박혜정, 이문현, 이상현, 이송민, 장정수한국미생물·생명공학회 · 한국미생물·생명공학회지2019 · 제47권 제4호벼 근권 유래 질소고정세균의 특성과항진균 활성김사열, 박유미, 설경조, 이수진, 정다은한국미생물·생명공학회 · 한국미생물·생명공학회지2006 · 제34권 제2호토양에서 분리한 Pseudomonas geniculata ANG3, Exiguobacterium acetylicum ANG40 및 Burkholderia stabilis ANG51의 식물 생장촉진 활성 및 식물병 방제활성김지윤, 김희숙, 박혜정, 이문현, 이상현, 이송민, 장정수한국미생물·생명공학회 · 한국미생물·생명공학회지2020 · 제48권 제1호환경스트레스와 관련된 indole-3-acetic acid 및 1-aminocylopropane-1-carboxylyic acid deaminase 활성을 갖는 박테리아의 분리와 특성 연구김지윤, 김희숙, 박혜정, 이문현, 이상현, 이송민, 장정수한국미생물·생명공학회 · 한국미생물·생명공학회지2019 · 제47권 제3호항진균성 활성 Bacillus sp.의 분리 및 생산 조건정용준한국환경생물학회 · 환경생물2009 · 제27권 제4호

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