본문 바로가기
KERD
논문KCI 등재

Suppression of Fusarium Wilt Caused by Fusarium oxysporum f. sp. lactucae and Growth Promotion on Lettuce Using Bacterial Isolates

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

This study was carried out to explore a non-chemical strategy for enhancing productivity by employing some antagonistic rhizobacteria. One hundred eighteen bacterial isolates were obtained from the rhizospheric zone of various crop fields of Gangwon-do, Korea, and screened for antifungal activity against Fusarium wilt (Fusarium oxysporum f. sp. lactucae) in lettuce crop under in vitro and in vivo conditions. In broth-based dual culture assay, fourteen bacterial isolates showed significant inhibition of mycelial growth of F. oxysporium f. sp. lactucae. All of the antagonistic isolates were further characterized for the antagonistic traits under in vitro conditions. The isolates were identified on the basis of biochemical characteristics and confirmed at their species level by 16S rRNA gene sequencing analysis. Arthrobacter sulfonivorans, Bacillus siamensis, Bacillus amyloliquefaciens, Pseudomonas proteolytica, four Paenibacillus peoriae strains, and Bacillus subtilis were identified from the biochemical characterization and 16S rRNA gene sequencing analysis. The isolates EN21 and EN23 showed significant decrease in disease severity on lettuce compared to infected control and other bacterial treatments under greenhouse conditions. Two bacterial isolates, EN4 and EN21, were evaluated to assess their disease reduction and growth promotion in lettuce in field conditions. The consortium of EN4 and EN21 showed significant enhancement of growth on lettuce by suppressing disease caused by F. oxysporum f. sp. lactucae respectively. This study clearly indicates that the promising isolates, EN4 (P. proteolytica) and EN21 (Bacillus siamensis), can be commercialized and used as biofertilizer and/or biopesticide for sustainable crop production.

Abstract

This study was carried out to explore a non-chemical strategy for enhancing productivity by employing some antagonistic rhizobacteria. One hundred eighteen bacterial isolates were obtained from the rhizospheric zone of various crop fields of Gangwon-do, Korea, and screened for antifungal activity against Fusarium wilt (Fusarium oxysporum f. sp. lactucae) in lettuce crop under in vitro and in vivo conditions. In broth-based dual culture assay, fourteen bacterial isolates showed significant inhibition of mycelial growth of F. oxysporium f. sp. lactucae. All of the antagonistic isolates were further characterized for the antagonistic traits under in vitro conditions. The isolates were identified on the basis of biochemical characteristics and confirmed at their species level by 16S rRNA gene sequencing analysis. Arthrobacter sulfonivorans, Bacillus siamensis, Bacillus amyloliquefaciens, Pseudomonas proteolytica, four Paenibacillus peoriae strains, and Bacillus subtilis were identified from the biochemical characterization and 16S rRNA gene sequencing analysis. The isolates EN21 and EN23 showed significant decrease in disease severity on lettuce compared to infected control and other bacterial treatments under greenhouse conditions. Two bacterial isolates, EN4 and EN21, were evaluated to assess their disease reduction and growth promotion in lettuce in field conditions. The consortium of EN4 and EN21 showed significant enhancement of growth on lettuce by suppressing disease caused by F. oxysporum f. sp. lactucae respectively. This study clearly indicates that the promising isolates, EN4 (P. proteolytica) and EN21 (Bacillus siamensis), can be commercialized and used as biofertilizer and/or biopesticide for sustainable crop production.

참고문헌 (51)

  1. Bano N, "Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent", Curr. Microbiol, 46, pp.324~328 (2003)
  2. Nelson EB, "Molecular approaches for understanding biological control mechanisms in bacteria : studies of interaction of Enterobacter cloacae with Pythium ultimum", Can. J. Plant Pathol, 14, pp.106~114 (1992)
  3. Armstrong, GM, "Fusarium: diseases, biology and taxonomy", pp.391~399 (1981), The Pennsylvania State University Press
  4. Ulloa, M, "Plant Disease Control: Towards Environmentally Acceptable Methods", pp.448~ (1993), Chapman and Hall
  5. Shanmugam V, "Biological management of vascular wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici by plant growth-promoting rhizobacterial mixture", Biol. Control, 57, pp.85~93 (2011)
  6. Cavender ND, "Vermicompost stimulates mycorrhizal colonization of roots of Sorghum bicolor at the expense of plant growth", Pedobiologia(Jena), 47, pp.85~89 (2003)
  7. Jetiyanon K, "Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases", Biol. Control, 24, pp.285~291 (2002)
  8. Turnbull G a, "The role of motility in the in vitro attachment of Pseudomonas putida PaW8 to wheat roots", FEMS Microbiol. Ecol, 35, pp.57~65 (2001)
  9. Dubey RC, "Enhancement of collar rot in sunflower caused by Sclerotinia rolfsii by Pseuodomonas", Indian Phytopathol, 58, pp.17~24 (2005)
  10. Zaved HK, "Isolation and characterization of effective bacteria for solid waste degradation for organic manure", KMITL J. Sci. Tech, 8, pp.44~55 (2008)
  11. Whipps JM, "Modern soil microbiology", pp.525~546 (1997), Marcel Dekker
  12. Arrebola E, "A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens", J. Appl. Microbiol, 108, pp.386~395 (2010)
  13. An Y, "Enhanced defense responses of tomato plants against late blight pathogen Phytophthora infestans by pre-inoculation with rhizobacteria", Crop Prot, 29, pp.1406~1412 (2010)
  14. Júnior VL, "Biocontrol of tomato late blight with the combination of epiphytic antagonists and rhizobacteria", Biol. Control, 38, pp.331~340 (2006)
  15. Akutsu K, "Growth inhibition of Botrytis spp. by Serratia marcescens B2isolated from tomato phylloplane", Ann. Phytopathol. Soc. Jpn, 59, pp.18~25 (1993)
  16. Sutton JC, "Biocontrol of Botrytis cinerea in strawberry leaves", Phytopathology, 83, pp.615~621 (1993)
  17. Paul B, "Suppression of Botrytis cinerea causing the grey mould disease of grape-vine by an aggressive mycoparasite, Pythium radiosum", FEMS Microbiol. Lett, 176, pp.25~30 (1999)
  18. Harley JL, "A method of studying active mycelia on living roots and other surfaces in the soils", Trans. Br. Mycol. Soc, 38, pp.104~118 (1975)
  19. Bharathi R, "Rhizobacteria based bio-formulations for the management of fruit rot infection in chillies", Crop Prot, 23, pp.835~843 (2004)
  20. Sheng Jun Xu, "Biocontrol of Fusarium Crown and Root Rot and Promotion of Growth of Tomato by Paenibacillus Strains Isolated from Soil", Mycobiology, 42(2), pp.158~166 (2014), 한국균학회
  21. Perneel M, "Characterization of CMR5c and CMR12a, novel fluorescent Pseudomonas strains from the cocoyam rhizosphere with biocontrol activity", J. Appl. Microbiol, 103, pp.1007~1020 (2007)
  22. Trivedi P, "Biological hardening of micropropagated Picrorhiza kurrooa Royel ex Benth., an endangered species of medical importance", World J. Microbiol. Biotechnol, 23, pp.877~878 (2007)
  23. Roberts WK, "Plant and bacterial chitinases differ in antifungal activity", J. Gen. Microbiol, 134, pp.169~176 (1988)
  24. Cappuccino JC, "Microbiology: a laboratory manual" (2006), Pearson Education, Inc
  25. Lorck H, "Production of hydrocyanic acid by bacteria", Physiol. Plant, 1, pp.142~146 (1948)
  26. Schwyn B, "Universal chemical assay for the detection and determination of siderophores", Anal. Biochem, 160, pp.47~56 (1987)
  27. Bric JM, "Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane", Appl. Environ. Microbiol, 57, pp.535~538 (1991)
  28. Goswami D, "Plant growth promoting potentials of Pseudomonas spp. strain OG isolated from marine water", J. Plant Interact, 8, pp.281~290 (2013)
  29. Weisburg WG, "16S ribosomal DNA amplification for phylogenetic study", J. Bacteriol, 173, pp.697~703 (1991)
  30. Reysenbach AL, "Differential amplification of rRNA genes by polymerase chain reaction", Appl. Environ. Microbiol, 58, pp.3417~3418 (1992)
  31. Tamura K, "MEGA6: molecular evolutionary genetics analysis version 6.0", Mol. Biol. Evol, 30, pp.2725~27299 (2013)
  32. Oh BJ, "Effect of cuticular wax layers of green and red pepper fruits on infection by Colletotrichum gloeosporioides", J. Phytopathol, 147, pp.547~552 (1999)
  33. Guo JH, "Biocontrol of tomato wilt by plant growth-promoting rhizobacteria", Biol. Control, 29, pp.66~72 (2004)
  34. IRRI, "CROPSTAT for Windows, version 7.2.3", ~ (2007)
  35. Metro Manila, "The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility", Biol. Fertil Soils, 37, pp.1~16 (2003)
  36. Atkinson A, "The beneficial rhizosphere : a dynamic entity", Appl. Soil Ecol, 48, pp.99~104 (2000)
  37. Garbeva P, "Microbial diversity in soil : selection of microbial populations by plant and soil type and implications for disease suppressiveness", Ann. Rev. Phytopathol, 42, pp.243~270 (2004)
  38. Paulitz TC, "Selection of rhizosphere bacteria for biological control of Pythium aphanidermatum on hydroponically grown cucumber", Biol. Control, 2, pp.226~237 (1992)
  39. Bakthavatchalu V, "Manganese superoxide dismutase is a mitochondrial fidelity protein that protects Polγ against UV-induced inactivation", Oncogene, 31, pp.2129~2139 (2011)
  40. Landa, BB, "Antagonistic activity of bacteria from the chickpea rhizosphere against Fusarium oxysporum f. sp. ciceris", Phytoparasitica, 25, pp.305~318 (1997)
  41. Saravanan T, "Effect of Pseudomonas fluorescens on fusarium wilt pathogen in banana rhizosphere", J. Biol. Sci, 4, pp.192~198 (2004)
  42. Ashwini N, "Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1", 3Biotech, 4, pp.127~136 (2014)
  43. Williams GE, "Selection of rhizobacteria for the control of Pythium ultimum and Aphanomyces cochlioides on sugarbeet seedlings", Crop Prot, 15, pp.479~486 (1996)
  44. Baker R, "Mechanisms of biological control of soil-borne pathogens", Annu. Rev. Phytopathol, 6, pp.263~294 (1968)
  45. Xu GW, "Selection of fluorescent pseudomonads antagonistic to Erwinia carotovora and suppressive of potato seed piece decay", Phytopathology, 76, pp.414~422 (1986)
  46. Majeed A, "Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion", Front. Microbiol, 6, pp.198~ (2015)
  47. Voisard C, "Cyanide production by Pseudomonas fluorescens helps suppress black root rot of tobacco under gnotobiotic conditions", EMBO J, 8, pp.351~358 (1989)
  48. Bashan Y, "How the plant growth-promoting bacterium Azospirillum promotes plant growth-a critical assessment", Adv. Agron, 108, pp.77~136 (2010)
  49. Muthamilan, M, "Integrated management of Sclerotium root rot of groundnut involving of Trichoderma harzianum, Rhizobium and Carbendazim", Indian J. Mycol. Plant Pathol, 26, pp.204~209 (1996)
  50. van Peer R, "Induced resistance and phytoalexin accumulation in biological control of fusarium wilt of carnation by Pseudomonas sp. Strain WCS417r.PDF", Phytopathology, 81, pp.728~734 (1991)
  51. Morrissey JP, "Exploitation of genetically modified inoculants for industrial ecology applications", Antonie Van Leeuwenhoek, 81, pp.599~606 (2002)

관련 논문AI추천

Effectiveness of Antagonistic Bacterial Metabolites to Control Rhizoctonia solani on Lettuces and Fusarium oxysporum on TomatoesGrosch Rita, Nguyen Ngoc Dung, Quyen Dinh Thi, Van Hanh Vu한국미생물·생명공학회 · 한국미생물·생명공학회지2013 · 제41권 제1호Bacillus amyloliquefaciens ISP-5 균주의 배지 최적화 및 상추를 이용한 식물 생장 촉진 평가김평일, 박해성, 서선일, 임지환, 최강현한국식물생명공학회 · Journal of Plant Biotechnology2022 · 제49권 제4호Bacillus spp.에 의한 황기의 생육촉진 및 토양병해 방제효과모영문, 이기욱, 이재형, 임수정, 조태희, 홍지은한국약용작물학회 · 한국약용작물학회지2021 · 제29권 제2호Profiles of Bacillus spp. Isolated from the Rhizosphere of Suaeda glauca and Their Potential to Promote Plant Growth and Suppress Fungal PhytopathogensChen Jia-Le, Chu Wan-Ying, Gu Zhou-Hang, Hao Ya-Qiao, Jiang Ke, Lu Ping, Xu Yu-Dong, Yang Jia-Yao, Zeng Guo-Hong, Zhao Hong-Xin한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2021 · 제31권 제9호Antagonistic and growth promotion potential of endophytic bacteria of mulberry (Morus spp.)Punathil Meethal Pratheesh Kumar, Sushma Ramesh, Thipperudraiah Thipeswamy, Venkadara Sivaprasad한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2015 · 제31권 제2호친환경 제제로부터 식물병원균에 대한 길항 미생물의 선발강근혜, 곽연식, 이옥순, 이용복, 차재율, 허빛나한국환경농학회 · 한국환경농학회지2012 · 제31권 제1호

이 간행물의 다른 논문 (9건)