황금으로부터 항산화 활성 성분의 분리
Isolation of Antioxidative Compound from Scutellaria baicalensis G.
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Abstract
: Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of 0.1 ㎍. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by 1H-NMR, 13C-NMR and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration 100 ㎍/3 ㎖. That was identified to be 3,5,7-trihydroxy-2'-methoxyflavanone by spectrophotometric analysis using 1H-NMR, 13C-NMR and Mass spectrophotometry.