생열귀나무 잎 추출물과 분획물의 항산화 및 NO생성 억제 활성
Anti-oxidant and Inhibitory Activity on NO Production of Extract and its Fractions from Rosa davurica Pall. Leaves
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초록
In the present study, we investigated biological activities of Rosa davurica Pall. leaves in order to evaluate thepossibility as a natural biomaterial. The 80% ethanol extract and its subsequent fractions of Rosa davurica Pall. leaves wereprepared using several solvents with different polarities. The extraction yield was 33.4, 36.6, 25.2, 18.7, 5.8 and 5.8% in ethanolextract, aqueous, butanol, ethyl acetate, n-hexane, chloroform fractions, respectively. The ethyl acetate fraction(661.38 ㎎/g), butanol fraction (396.68 ㎎/g) and 80% ethanolic extract (239.54 ㎎/g) has higher total polyphenol contentsthan other fractions. The antioxidant activity was detected in ethanolic extract, ethyl acetate and butanol fractions. Theethyl acetate fraction showed the highest levels of DPPH radical scavenging activity (IC50, 4.77 ㎍/㎖). Moreover, the ethylacetate fraction significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results indicate that 80% ethanol extract and its fractions of Rosa davurica Pall. leaf, especially ethyl acetate fraction,have the properties of anti-oxidant and anti-inflammation, suggesting leaf of Rosa davurica Pall. may be a candidate fornatural and functional materials.
Abstract
In the present study, we investigated biological activities of Rosa davurica Pall. leaves in order to evaluate thepossibility as a natural biomaterial. The 80% ethanol extract and its subsequent fractions of Rosa davurica Pall. leaves wereprepared using several solvents with different polarities. The extraction yield was 33.4, 36.6, 25.2, 18.7, 5.8 and 5.8% in ethanolextract, aqueous, butanol, ethyl acetate, n-hexane, chloroform fractions, respectively. The ethyl acetate fraction(661.38 ㎎/g), butanol fraction (396.68 ㎎/g) and 80% ethanolic extract (239.54 ㎎/g) has higher total polyphenol contentsthan other fractions. The antioxidant activity was detected in ethanolic extract, ethyl acetate and butanol fractions. Theethyl acetate fraction showed the highest levels of DPPH radical scavenging activity (IC50, 4.77 ㎍/㎖). Moreover, the ethylacetate fraction significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results indicate that 80% ethanol extract and its fractions of Rosa davurica Pall. leaf, especially ethyl acetate fraction,have the properties of anti-oxidant and anti-inflammation, suggesting leaf of Rosa davurica Pall. may be a candidate fornatural and functional materials.
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