일천궁에서 분리한 근권 내생 미생물의 식물생육증진 효과
Rhizobacterial Endophytes from Cnidium officinale Makino for Plant Growth Promoting Effect
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Background: This study was conducted to evaluate the plant growth promoting effect of rhizobac- terial endophytes on Cnidium officinale Makino cultivated in Korea. Methods and Results: Twenty-four rhizobacterial were isolated from C. officinale Makino, and the L2 strain observed to have excellent plant growth promotion ability. Based on 16s rRNA sequence analysis, L2 was identified as Enterobacter ludwigii. The selected endophyte was evalu- ated for indole-3-acetic acid (IAA), siderophore, phosphate solubilizing assay, and extracellular enzyme activity to determine the growth promoting effect of the strain on C. officinale Makino under pot conditions. L2 produced IAA and siderophore and exhibited phosphate solubilizing activity. L2 was inoculated to the pot to evaluate the growth of C. officinale Makino. The shoot fresh weight and rhizome dryweight of C. officinale Makino inoculated with L2 after 5 weeks were higher than those of control samples. Conclusions: This study suggests that rhizobacterial endophytes can be used as a growth promoter and may be a potential for biofertilizer in the cultivation of Cnidium officinale Makino.
Abstract
Background: This study was conducted to evaluate the plant growth promoting effect of rhizobac- terial endophytes on Cnidium officinale Makino cultivated in Korea. Methods and Results: Twenty-four rhizobacterial were isolated from C. officinale Makino, and the L2 strain observed to have excellent plant growth promotion ability. Based on 16s rRNA sequence analysis, L2 was identified as Enterobacter ludwigii. The selected endophyte was evalu- ated for indole-3-acetic acid (IAA), siderophore, phosphate solubilizing assay, and extracellular enzyme activity to determine the growth promoting effect of the strain on C. officinale Makino under pot conditions. L2 produced IAA and siderophore and exhibited phosphate solubilizing activity. L2 was inoculated to the pot to evaluate the growth of C. officinale Makino. The shoot fresh weight and rhizome dryweight of C. officinale Makino inoculated with L2 after 5 weeks were higher than those of control samples. Conclusions: This study suggests that rhizobacterial endophytes can be used as a growth promoter and may be a potential for biofertilizer in the cultivation of Cnidium officinale Makino.
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