독도의 벼과식물로부터 분리된 Enterobacter spp.에 의한 고추의 흰별무늬병에 대한 전신유도저항성
Induced of Systemic Resistance against Gray Leaf Spot in Pepper by Enterobacter Species Isolated from Family Gramineae Plants in Dok-do
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초록
본 연구는 독도에 자생하는 개밀의 야생군락지의 근권으로부터 분리된 균 중 식물의 생장을 촉진하고, 식물의 병원균을 억제하는 것으로 알려져 있는 Enterobacter 속 세균에초점을 맞추어 실험을 진행하였다. 개밀 근권으로부터 총124종의 세균을 분리하였으며 16S rDNA sequence를 이용하여 부분동정한 결과, 13종의 Enterobacter 속 세균을 확인할 수 있었다. 분리된 Enterobacter 속 세균들의 식물생장촉진 근권세균으로서의 특징을 밝히기 위해 식물의 생장호르몬인 auxin의 생산능력과 난용성인의 가용능, siderophore 생산능을 측정하였다. 식물생장호르몬인 auxin을 형성하는 균주12종, 난용성 인을 분해할 수 있는 균주 12종, siderophore를형성할 수 있는 균주 11종이 각각 확인되었다. 분리된 균주들은 대표적인 식용작물인 고추에 직접 적용하여 식물의 생장촉진 효과와 병원균에 대한 전신유도저항성을 확인하였다. 그 중 KUDC5002와 KUDC5013의 경우 식물의 생장을 촉진시키는 균주로 확인되었다. 특히 KUDC5007, KUDC5008및 KUDC5010의 경우, 지금까지 보고된 바 없었던 고추 유묘의 흰별무늬병에 대한 식물의 저항성을 증가시키는 것으로 확인되어 새로운 생물학적 제재로서 매우 가치 있을 것으로 기대된다.
Abstract
This study’s aim is to isolate and characterize plant growth promoting Enterobacter species for the biological control of gray leaf spot in pepper. Screening was carried out from the rhizosphere of Agropyron tsukushiensi var. transiens (Hack.) Ohwi in Dok-do. Rhizobacterial isolates were partially identified by 16S rDNA sequencing and Enterobacter species were tested for plant growth promoting capabilities and the induction of systemic resistance in pepper against gray leaf spot caused by Stemphylium solani. Isolates were tested for production of indole-acetic acid and siderophore, and for phosphate solubilization. The application of isolates was effective in controlling gray leaf spot in pepper with E. asburiae (KNUC5007) and E. cancerogenes (KNUC5008 and KNUC5010) having the highest efficacy in reducing gray leaf spot severity. This is the first report of the biological control of gray leaf spot in pepper using rhizobacteria and it is hoped that this study will increase the utilization of Enterobacter species as plant growth promoters and biocontrol agents.
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