Auxin과 ACC Deaminase를 생산하는 사구식물 복원용 근권세균의 선발
Selection of the Auxin and ACC Deaminase Producing Plant Growth Promoting Rhizobacteria from the Coastal Sand Dune Plants.
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In order to develop the multi-functional rhizobacteria that can exert positive effect on the growth of plants growing in the coastal sand dune located along East Coast of Korea, rhizospheral bacteria of 11 different plants from this area were isolated 1,330 rhizobacteria. Among these, 23 strains were able to produce auxin and had spectrum of antagonism toward various phytopathogenic microbes. To know the mechanism of this antifungal activity, these 23 strains were subjected to further analyses; 19 strains of these produced siderophore as determined by color reaction on CAS-blue plate, 4 strains produced antifungal cellulase as judged by color change on CMC-Congo red plate, 17 strains were able to utilized insoluble phosphate salts, also determined by clear zone formation on PVK medium. Identification of the strain was assigned to all 23 strains by 16s rDNA sequence analysed, and all were identified to be in the genus of Bacillus and Pseudomonas. One strain of these, denoted Pseudomonas fluorescens IB4-14, showed ACC deaminase activity which is known to be involved in the resistance of environmental stress such as salt and drought. Also, P. fluorescens IB4-14 showed the germination stimulation and roots growth promoting activity on the in vivo assay of Lysimachia mauritiana Lam. (spoonleaf yellow loosestrife).
Abstract
In order to develop the multi-functional rhizobacteria that can exert positive effect on the growth of plants growing in the coastal sand dune located along East Coast of Korea, rhizospheral bacteria of 11 different plants from this area were isolated 1,330 rhizobacteria. Among these, 23 strains were able to produce auxin and had spectrum of antagonism toward various phytopathogenic microbes. To know the mechanism of this antifungal activity, these 23 strains were subjected to further analyses; 19 strains of these produced siderophore as determined by color reaction on CAS-blue plate, 4 strains produced antifungal cellulase as judged by color change on CMC-Congo red plate, 17 strains were able to utilized insoluble phosphate salts, also determined by clear zone formation on PVK medium. Identification of the strain was assigned to all 23 strains by 16s rDNA sequence analysed, and all were identified to be in the genus of Bacillus and Pseudomonas. One strain of these, denoted Pseudomonas fluorescens IB4-14, showed ACC deaminase activity which is known to be involved in the resistance of environmental stress such as salt and drought. Also, P. fluorescens IB4-14 showed the germination stimulation and roots growth promoting activity on the in vivo assay of Lysimachia mauritiana Lam. (spoonleaf yellow loosestrife).
참고문헌 (22)
- Bhat, K. M., "Sporotrichum thermophile growth, cellulose degration and cellulase activity", Appl. Env. Microbio, 53, pp.2175~2182 (1987)
- Chio, D. W., "Respection of pectic enzyme among the hydrolysis enzymes of plant cell wall", Korean J. Food & Nutrition, 9, pp.92~101 (1996)
- Chun, J. O., "Effect of cell wall hydrolase and Ca++ hydrolysis of isolated apple cell wall", J. Kor. Soc. Hort. Sci, 13, pp.206~207 (1995)
- Devender K. J., "Characterization of a substance produced by Azospirillum which causes branching of wheat root hairs", Can. J. Microbiol, 31, pp.206~210 (1984)
- Elad, Y., "Influence of trace amounts of cautions and siderophore producing Pseudomonads on chlamydospore germination of Fusarium oxysprum", Phytopathol, 75, pp.1047~1052 (1985)
- Glick B. R, "Phytomediation: synergistic use of plants and bacteria to clean the environment", Biotechnol. Adv, 2, pp.383~393 (2003)
- Holt, J. G., "Bergey's manual of determinative bacteriology. 9th" (1994), Williams and Wilkins
- KATIYAR, "Improved Plant Growth fromSeed Bacterization Using SiderophoreOverproducing Cold ResistantMutant of Pseudomonas fluorescens", Journal of Microbiology and Biotechnology, 14(4), pp.653~657 (2004), 한국미생물·생명공학회
- 권도형, "Auxin과 항진균물질을 생산하는 식물생장촉직근권세균의 분리동정 및 특성", Journal of the Korean Society for Applied Biological Chemistry, 47(1), pp.17~21 (2004), 한국응용생명화학회
- Kowalchuk, G. A., "Community analysis of arbuscular mycorrhizal fungi associated with Ammophila arenaria in Dutch coastal sand dunes", Mol. Ecol, 11, pp.571~581 (2002)
- Lee, J. M., "Isolation of siderophore-producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease", or. J. Appl. Microbiol. Biotech, 27, pp.427~432 (1999)
- Lee, M. S., "Dominance of Lysobacter sp. in the rhizosphere of two coastal sand dune plant species, Calystegia soldanella and Elymus mollis", Antonie Leeuwenhoek, 90, pp.19~70 (2006)
- Lim, S. U., "Isolation and physiological characteristics of auxin-producing soil bacteria", Korean J. Soil Sci. Fert, 28, pp.75~82 (1995)
- Opelt, K, "Diversity and antagonistic potential of bateria associated with bryophytes from nutrientpoor habitats of Baltic sea coast", Appl. Environ. Microbiol, 70, pp.6569~6579 (2004)
- Park, M. S., "Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanese sp. nov., isolated from roots of sand-dune plants", Int. J. Syst. Evol. Microbiol, 56, pp.433~438 (2006)
- Reva, O. N., "Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis", FEMS Microbiol. Ecol, 48, pp.249~259 (2004)
- Seong, K. Y., "Factors influencing siderophore production by plant growth promoting rhizopseudomonas strains", Korean J. Soil Sci. Fert, 28, pp.287~294 (1995)
- SHIN, DONG-SUNG, "Plant Growth-Promoting Potential of Endophytic Bacteria Isolated from Roots of Coastal Sand Dune Plants", Journal of Microbiology and Biotechnology, 17(8), pp.1361~1368 (2007), 한국미생물·생명공학회
- Shivanna, M. B., "Sterile fungi from zoysia grass rhizosphere as plant growth promoters in spring wheat", Can. J. Microbiol, 40, pp.637~644 (1994)
- Vyas, Pratilbha, "Phosphate solublization potentail and stress tolernace of Eupenicillium parvum from tea soil", Microbiological Research, 통권 : 167, pp.931~938 (2007)
- Waring, W. S., "Growth of bacteria in an iron-free medium", Arch. Biochem, 1, pp.303~310 (1942)
- Weinberg, E. D, "Iron and susceptibility to infectious disease", Science, 통권 : 184, pp.952~956 (1974)