Quantitative Analysis of 1-Deoxynojirimycin Content Using Silkworm Genetic Resources
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초록
1-Deoxynojirimycin(1-DNJ or DNJ), a component in silkworm powder, prevents glucose frombeing absorbed into the bloodstream from the small intestine by inhibiting a-glucosidaseactivity. This study compared the functional components of 1-DNJ from various silkwormspecies using a gene database with those of 1-DNJ produced by silkworms bred throughcross-combinations. We utilized comparisons of geographical origins and species ofsilkworms using a gene database and discovered that 1-DNJ activity was ranked in thefollowing order by species, Japanese (SK-1) > Chinese (C48) > European (Rock191). 1-DNJconstituted varying percentages of silkworm organs in the following order, blood > epithelialtissue > body fat > silk glands. With regard to sex, 1-DNJ, levels were higher in males thanfemales. However, 1-DNJ levels with respect to various genetic traits (blood color, silk color,and egg color) were consistent. In order to study 1-DNJ changes that occurred during crossbreeding of the silkworm gene, we bred cross-combinations utilizing SK-1 and C48 silkworms. In conclusion, in order to provide information about the constituents of functional materialscontained in silkworm powder, it is imperative that silkworm cross breeding occurs so that thedatabase of functional materials extracted from silkworms will expand.
Abstract
1-Deoxynojirimycin(1-DNJ or DNJ), a component in silkworm powder, prevents glucose frombeing absorbed into the bloodstream from the small intestine by inhibiting a-glucosidaseactivity. This study compared the functional components of 1-DNJ from various silkwormspecies using a gene database with those of 1-DNJ produced by silkworms bred throughcross-combinations. We utilized comparisons of geographical origins and species ofsilkworms using a gene database and discovered that 1-DNJ activity was ranked in thefollowing order by species, Japanese (SK-1) > Chinese (C48) > European (Rock191). 1-DNJconstituted varying percentages of silkworm organs in the following order, blood > epithelialtissue > body fat > silk glands. With regard to sex, 1-DNJ, levels were higher in males thanfemales. However, 1-DNJ levels with respect to various genetic traits (blood color, silk color,and egg color) were consistent. In order to study 1-DNJ changes that occurred during crossbreeding of the silkworm gene, we bred cross-combinations utilizing SK-1 and C48 silkworms. In conclusion, in order to provide information about the constituents of functional materialscontained in silkworm powder, it is imperative that silkworm cross breeding occurs so that thedatabase of functional materials extracted from silkworms will expand.
참고문헌 (21)
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