Effect of pH values and inoculation amounts for a-glucosidase inhibitory activity in mulberry leaf fermentation
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Mulberry leaves containing 1-deoxynojirimycin (DNJ) have been recognized as a potentially important source for prevent or treat hyperglycemia. However, DNJ content of natural mulberry leaf are as low as 0.1%. Thus, the most effective method for increasing a-glucosidase inhibitory activity with the DNJ high-production is needed. In this study, we investigated the influence of a-glucosidase inhibitory activity according to different pH values (6-9) and inoculation amounts (0.1-0.5%) when Bacillus subtilis cultured on mulberry leaf powder media. We confirmed that a-glucosidase inhibitory activity was difference according to culture conditions of different pH values, inoculation amounts, and fermentation times. The results of mulberry leaf fermentation according to pH values and inoculation amounts were shown that the optimal conditions for a-glucosidase inhibitory activity were defined as pH 7 and 9, inoculation amount 0.4%, and incubation until 2 to 4 days. These results can be provided a basic data for the optimal culture conditions increasing a-glucosidase inhibitory activity from mulberry leaf fermentation.
Abstract
Mulberry leaves containing 1-deoxynojirimycin (DNJ) have been recognized as a potentially important source for prevent or treat hyperglycemia. However, DNJ content of natural mulberry leaf are as low as 0.1%. Thus, the most effective method for increasing a-glucosidase inhibitory activity with the DNJ high-production is needed. In this study, we investigated the influence of a-glucosidase inhibitory activity according to different pH values (6-9) and inoculation amounts (0.1-0.5%) when Bacillus subtilis cultured on mulberry leaf powder media. We confirmed that a-glucosidase inhibitory activity was difference according to culture conditions of different pH values, inoculation amounts, and fermentation times. The results of mulberry leaf fermentation according to pH values and inoculation amounts were shown that the optimal conditions for a-glucosidase inhibitory activity were defined as pH 7 and 9, inoculation amount 0.4%, and incubation until 2 to 4 days. These results can be provided a basic data for the optimal culture conditions increasing a-glucosidase inhibitory activity from mulberry leaf fermentation.
참고문헌 (36)
- Anno T, "Maltase, sucrase and α-amylase inhibitory activity of Morus leaves extract", Food Preserv Sci, 30, pp.223~229 (2004)
- Asai A, "Effect of mulberry leaf extract with enriched 1-deoxynojirimycin content on postprandial glycemic control in subjects with impaired glucose metabolism", J Diabetes Investig, 2, pp.318~323 (2011)
- Asano N, "Sugars with nitrogen in the ring isolated from the leaves of Morus bombycis", Carbohydr Res, 253, pp.235~245 (1994)
- Asano N, "Polyhydroxylated alkaloids isolated from mulberry trees(Morus alba L. )and silkworms(Bombyx mori L. )", J Agric Food Chem, 49, pp.4208~4213 (2001)
- Bharani SE, "Immunomodulatory activity of methanolic extract of Morus alba Linn. (mulberry)leaves", Pak J Pharm Sci, 23(1), pp.63~68 (2010)
- Chai OH, "Inhibitory effects of Morus alba on compound 48", 80-induced anaphylactic reactions and anti-chicken gamma globulin IgE-mediated mast cell activation, 2005, pp.28(10), 1852~1858
- Choi EM, "Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264. 7 macrophages", Fitoterapia, 76, pp.608~613 (2005)
- Dimo T, "Effects of leaf aqueous extract of Bidens pilosa(Asteraceae)on KCl-and norepinephrine-induced contractions of rat aorta", J thnopharmacol, 60(2), pp.179~182 (1998)
- Floris AV, "α-glucosidase inhibitors for patients with type 2 diabetes", Diabetes Care, 28, pp.154~162 (2005)
- Fukai T, "Structures of two natural hypotensive diels-alder type adducts, mulberrofuran F and G, from the cultivated mulberry tree (Morus lhou Koidz)", Chem Pharm Bull (Tokyo), 33, pp.3195~3204 (1985)
- Gui ZZ, "Studies on the edibility and therapeutic effects of the silkworm powder. Acta Sericol", Sinica, 30, pp.107~110 (2004)
- Holman RR, "Assessing the potential for α-glucosidase inhibitors in prediabetic states", Diabetes Res Clin Pract, 40, pp.s21~s25 (1998)
- 정종훈, "Enhancement of 1-Deoxynojirimycin Content and α-Glucosidase Inhibitory Activity in Mulberry Leaf Using Various Fermenting Microorganisms Isolated from Korean Traditional Fermented Food", Biotechnology and Bioprocess Engineering, 19(6), pp.1114~1118 (2014), 한국생물공학회
- Jiang YG, "Improved 1-Deoxynojirimycin(DNJ)production in mulberry leaves fermented by microorganism", Braz J Microbiol, 45, pp.721~729 (2014)
- 주완택, "Screening of 1-deoxynojirimycin (DNJ) producing bacteria using mulberry leaf", International Journal of Industrial Entomology, 31(2), pp.48~55 (2015), 한국잠사학회
- 주완택, "Comparison of optimal temperature and time conditions for highest a-glucosidase inhibitory activity from various of Korea mulberry teas", International Journal of Industrial Entomology, 33(1), pp.31~35 (2016), 한국잠사학회
- KIM,KYOUNG-JA, "Production of a-Glucosidase Inhibitor by b-Glucosidase Inhibitor-Producing Bacillus lentimorbus B-6", Journal of Microbiology and Biotechnology, 12(6), pp.895~900 (2002), 한국미생물·생명공학회
- Kim SY, "Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia rats", J Korean Soc Food Sci Nutr, 27, pp.1217~1222 (1998)
- 김용순, "Optimization of culture conditions of Bacillus subtilis with α-glucosidase inhibitory activity", International Journal of Industrial Entomology, 33(1), pp.24~30 (2016), 한국잠사학회
- Kimura M, "Antihyperglycemic effects of N-containing sugars derived from mulberry leaves in streptozocin-induced diabetic mice", J Trad Med, 12, pp.214~219 (1995)
- "Development of Mulberry Leaf Extract for Suppressing Postparandial Blood Glucose Elevation" (2011)[URL]
- Kimura T, "Determination of 1‐deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection", J Agric Food Chem, 52, pp.1415~1418 (2004)
- Kojima Y, "Effects of mulberry leaf extract rich in 1-deoxynojirimycin on blood lipid profiles in humans", J Clin Biochem Nutr, 47, pp.155~161 (2010)
- Miyahara C, "Inhibitory effect mulberry leaf extract on postprandial hyperglycemic in normal rats", J Nutritional Sci Vitaminol, 50, pp.161~164 (2004)
- Nakanishi H, "Effect of environmental conditions on the α-glucosidase inhibitory activity of mulberry leaves", Biosci Biotech Bioch, 75, pp.2293~2296 (2011)
- Naowaboot J, "Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract instreptozotocininduced chromic diabetic rats", Plant Foods Hum Nutr, 64, pp.116~121 (2009)
- Oh H, "Hepatoprotective and free radical scavenging activities of prenylflavonoids, coumarin, and stilbene from Morus alba", Planta Med, 68(10), pp.932~934 (2002)
- Oku T, "Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccharidase activity", Br J Nutr, 95, pp.933~938 (2006)
- Sastry CR, "Mulberry varieties, exploitation and pathology", Sericologia, 24, pp.333~359 (1984)
- Schmidt DD, "α-Glucosidase inhibitors, new complex oligosaccharides of microbial origin", Naturwissenschaften, 64, pp.535~536 (1977)
- Shibano M, "Biosynthesis of 1-deoxynojirimycin in Commelina communis: A difference between the microorganisms and plants", Phytochem, 65, pp.2661~2665 (2004)
- Singab AN, "Hypoglycemic effect of Egyptian Morus alba root bark extract : effect on diabetes and lipid peroxidation of streptozotocin-induced diabetic rats", J Ethnopharmacol, 100(3), pp.333~338 (2005)
- Stein DC, "Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin", Appl Environ Microbiol, 48, pp.280~284 (1984)
- Vichasilp C, "Optimization of 1-deoxynojirimycin extraction from mulberry leaves by using response surface methodology", Biosci Biotechnol Biochem, 73, pp.2684~2689 (2009)
- Yamaki K, "Evaluation of alpha-glucosidase inhibitory activity in colored foods : A trial using slope factors of regression curves", Nippon Shokuhin Kagaku Kaishi, 53, pp.229~231 (2006)
- Yatsunami K, "The relationship between 1and deoxynojirimycin content and α-glucosidase inhibitory activity in leaves of 276 mulberry cultivars(Morus spp. )in Kyoto, Japan", J Nat Med, 62, pp.63~66 (2008)