Effects of Physiological Active Substance Extracted from Silkworm Fece
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초록
Silkworm (Bombyx mori ) feces have long been used in the pharmaceutical and food industriesas a natural colorant. However, there is limited data on the bioactive compounds thatconstitute silkworm feces. This research emphasizes the antioxidant activity of different solventand flavonoid extracts of silkworm feces. The solvents were ethanol, butanol, and water, whilethe methods utilized included ultrasonification, stirrer, reflux, and reflux after ultrasonificationextraction. Results showed that butanol ultrasonification extraction (BUE) yield the lowestextraction (1.75%), while the other methods yielded 7 to 14%. The total polyphenol contentutilizing BUE was 3.3 mg TAE/g, while water ultrasonification extraction (WUE) yielded thehighest extraction rate with 51.6 mg TAE/g. The total flavonoid content was significantly higherusing ethanol reflux extraction (EUE) at 266.8 mg QRE/g BUE, which was 158.3 and 151.3mg QRE/g. Both DPPH radical scavenging activity and SOD-like (superoxide dismutase)activity, showed significant antioxidant effects. Finally, all other extracts except for BUE hada-glucosidase inhibition at 60%. Therefore, an effective extraction method for physiologicallyactive substances must be selected.
Abstract
Silkworm (Bombyx mori ) feces have long been used in the pharmaceutical and food industriesas a natural colorant. However, there is limited data on the bioactive compounds thatconstitute silkworm feces. This research emphasizes the antioxidant activity of different solventand flavonoid extracts of silkworm feces. The solvents were ethanol, butanol, and water, whilethe methods utilized included ultrasonification, stirrer, reflux, and reflux after ultrasonificationextraction. Results showed that butanol ultrasonification extraction (BUE) yield the lowestextraction (1.75%), while the other methods yielded 7 to 14%. The total polyphenol contentutilizing BUE was 3.3 mg TAE/g, while water ultrasonification extraction (WUE) yielded thehighest extraction rate with 51.6 mg TAE/g. The total flavonoid content was significantly higherusing ethanol reflux extraction (EUE) at 266.8 mg QRE/g BUE, which was 158.3 and 151.3mg QRE/g. Both DPPH radical scavenging activity and SOD-like (superoxide dismutase)activity, showed significant antioxidant effects. Finally, all other extracts except for BUE hada-glucosidase inhibition at 60%. Therefore, an effective extraction method for physiologicallyactive substances must be selected.
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