Heat Stability and Glucose-Lowering Effect of 1-Deoxynojirimycin from Silkworm (Bombyx mori) extract Powder
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초록
Silkworm powder, which contains 1-deoxynojirimycin (DNJ), is a promising complementaryand alternative medicine (CAM) in Korea. Silkworm powder was produced from Yeonnokjampupae at d 3 of the 5th instar at the National Academy of Agricultural Science. The powderwas derivatized with 9-fluorenylmethyl chloroformate (FMOC-Cl), and the DNJ-FMOC contentwas measured by HPLC. We investigated the content of 1-DNJ in the silkworm powder andits glucose-lowering effect when it was treated at different temperatures. The content of 1-DNJwas the lowest at 150°C, while it was constant at other temperatures. The silkworm extractpowder was orally administered to diabetic mice (20 mg/kg/d) for 4 wk. Water intake did notsignificantly change when compared with the control group (T0). The blood glucose levelssignificantly decreased when mice where administered silkworm powder treated at 60°C (T60)compared to the control group, but no difference was observed between the groups T100and T150. Moreover, the blood levels of TG significantly decreased compared with the controlgroup. Based on these results, we surmise that the properties of the silkworm extract powderwere stable upon heating at 100°C but not at 150°C.
Abstract
Silkworm powder, which contains 1-deoxynojirimycin (DNJ), is a promising complementaryand alternative medicine (CAM) in Korea. Silkworm powder was produced from Yeonnokjampupae at d 3 of the 5th instar at the National Academy of Agricultural Science. The powderwas derivatized with 9-fluorenylmethyl chloroformate (FMOC-Cl), and the DNJ-FMOC contentwas measured by HPLC. We investigated the content of 1-DNJ in the silkworm powder andits glucose-lowering effect when it was treated at different temperatures. The content of 1-DNJwas the lowest at 150°C, while it was constant at other temperatures. The silkworm extractpowder was orally administered to diabetic mice (20 mg/kg/d) for 4 wk. Water intake did notsignificantly change when compared with the control group (T0). The blood glucose levelssignificantly decreased when mice where administered silkworm powder treated at 60°C (T60)compared to the control group, but no difference was observed between the groups T100and T150. Moreover, the blood levels of TG significantly decreased compared with the controlgroup. Based on these results, we surmise that the properties of the silkworm extract powderwere stable upon heating at 100°C but not at 150°C.
참고문헌 (17)
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