Screening of 1-deoxynojirimycin (DNJ) producing bacteria using mulberry leaf
2
조회
0
다운로드
8
인용
0
참고문헌
키워드
초록
1-Deoxynojirimycin (DNJ) has been extensively investigated for its applications as an a-glucosidase inhibitor in postprandial hyperglycemia, and has been applied to nutraceuticals and medicines to prevent or delay the progression of type 2 diabetes. However, the amount of DNJ in mulberry leaves is low (approximately 0.1%), therefore, a more effective extraction method is needed. In this study, microbial DNJ production was developed as an alternative to chemical methods. We identified fermented sericultural products and bacteria that produce DNJ in large quantities using high performance liquid chromatography and thin layer chromatography. The inhibition of a-glucosidase activity was examined with respect to DNJ production or non-production. Crude DNJ from the isolated strains exhibited greater than 70% a-glucosidase activity. An investigation of the effect of mulberry leaf powder concentration (1~5%), using high DNJ producing bacteria, provided evidence for microbial mass production of DNJ
Abstract
1-Deoxynojirimycin (DNJ) has been extensively investigated for its applications as an a-glucosidase inhibitor in postprandial hyperglycemia, and has been applied to nutraceuticals and medicines to prevent or delay the progression of type 2 diabetes. However, the amount of DNJ in mulberry leaves is low (approximately 0.1%), therefore, a more effective extraction method is needed. In this study, microbial DNJ production was developed as an alternative to chemical methods. We identified fermented sericultural products and bacteria that produce DNJ in large quantities using high performance liquid chromatography and thin layer chromatography. The inhibition of a-glucosidase activity was examined with respect to DNJ production or non-production. Crude DNJ from the isolated strains exhibited greater than 70% a-glucosidase activity. An investigation of the effect of mulberry leaf powder concentration (1~5%), using high DNJ producing bacteria, provided evidence for microbial mass production of DNJ
참고문헌 (31)
- Asai A, "Effect of mulberry leaf extract with enriched 1-deoxynojirimycin content on postprandial glycemic control in subjects with impaired glucose metabolism", J. Diabetes. Investig, 2, pp.318~323 (2011)
- Asano N, "Polyhydroxylated alkaloids isolated from mulberry trees (Morus alba L.) and silkworms (Bombyx mori L.)", J. Agric. Food Chem, 49, pp.4208~4213 (2001)
- Asano N, "Glycosidase inhibitors: update and perspectives on practical use", Glycobiol., 13, pp.93R~104R (2003)
- Daniel CS, , Appl. Environ. Microbiol, 48(2), pp.280~284 (1984)
- Ezure Y, "Moranoline (1-deoxynojirimycin) fermentation and its improvement", Agric. Biol. Chem., 49, pp.1119~1125 (1985)
- Floris AV, "α-Glucosidase inhibitors for patients with type 2 diabetes", Diabetes Care, 28, pp.154~162 (2005)
- Fujita H, "Efficacy and safety of Touchi extract, an α-glucosidase inhibitor derived from fermented soybeans, in non-insulin-dependent diabetic mellitus", J. Nutr. Biochem, 12, pp.351~356 (2001)
- Fujita H, "Long-term ingestion of Touchi-extract, an α-glucosidase inhibitor, by borderline and mild type-2 diabetic subjects is safe and significantly reduces blood glucose levels", Nutr. Res, 23, pp.713~722 (2003)
- Han J, "Effects of silkworm powder on glucose absorption by human intestinal epithelial cell line Caco-2", J. Nat. Med., 61(4), pp.387~390 (2007)
- Hardick DJ, "The biosynthesis of deoxynojirimycin and deoxymannonojirimycin in Streptomyces subrutilus", J. Chem. Soci. & Chem. Communications., 10, pp.729~730 (1991)
- Hardick D, "The biosynthesis of 1-deoxynojirimycin in Bacillus subtilis var niger", Tetrahedron, 49, pp.6707~6716 (1993)
- Herscovics A, "Importance of glycosidases in mammalian glycoprotein biosynthesis", Biochim. Biophys. Acta., 1473, pp.96~107 (1999)
- Horton ES, "NIDDM-the devastating disease", Diabetes. Res. Clin. Pract, 28, pp.S3~S11 (1995)
- Kim JW, "Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversed-phase high-performance liquid chromatography", J. Chromatogr. A., 1002, pp.93~99 (2003)
- Kimura T, "Determination of 1-deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection", J. Agric. Food Chem., 52, pp.1415~1418 (2004)
- Kimura T, "Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in human", J. Agric. Food Chem., 55, pp.5869~5874 (2007)
- Konno K, "Mulberry latex rich in antidiabetic sugar-mimic alkaloids forces dieting on caterpillars", Proceedings of National Academy of Sciences in USA, 103, pp.1337~1341 (2006)
- McCue P, "Anti-diabetic and antihypertensive potential of sprouted and solid-state bioprocessed soybean", Asian Pacific Journal of Clinic Nutrition, 14(2), pp.145~152 (2005)
- Murata K, "Effect of propolis and mulberry leaf extract (Quapolis Tm) on type 2 diabetes", Food Func., 1(1), pp.26~30 (2005)
- Paek NS Kang DJ, "Production of 1-deoxynojirimycin by Streptomyces sp. SID9135", J. Microbiol. Biotechnol., 7, pp.262~266 (1997)
- Romaniouk AV, "Synthesis of a novel photoaffinity derivative of 1-deoxynojirimycin for active sitedirected labeling of glucosidase I", Glycobiology, 2004(14), pp.301~310 (2004)
- Ryu KS, "An activity of lowering blood-glucose levels according to preparative conditions of silkworm powder", Korean J. Seric. Sci, 39, pp.79~85 (1997)
- 류강선, "1-Deoxynojirimycin Content and Blood Glucose-Lowering Effect of Silkworm (Bombyx mori) Extract Powder", International Journal of Industrial Entomology, 27(2), pp.237~242 (2013), 한국잠사학회
- Shibano M, "Biosynthesis of 1-deoxynojirimycin in Commelina communis: A difference between the microorganisms and plants", Phytochem., 65, pp.2661~2665 (2004)
- Stein DC, "Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin", Appl. Environmental. Microbiol, 48, pp.280~284 (1984)
- Vichasilp C, "A novel gelatin crosslinking method retards release of mulberry 1-deoxynojirimycin providing a prolonged hypoglycaemic effect", Food Chem., 134, pp.1823~1830 (2012)
- Yagi M, "The structure of moranoline, a piperidine alkaloid from Morus species", Nippon Nogeikagaku Kaishi, 50, pp.571~572 (1976)
- Yamaki K, "Evaluation of alpha-glucosidase inhibitory activity in colored foods: A trial using slope factors of regression curves", Nippon Shokuhin Kagaku Kaishi, 53, pp.229~231 (2006)
- Yatsunami K, "1-Deoxynojirimycin content and alfa-Glucosidase inhibitory activity and heat stability of 1-deoxynojirimycin in silkworm powder", Food. Nutri. Sci., 2, pp.87~89 (2011)
- Yin H, "Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L", Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 11(4), pp.286~291 (2010)
- Zheng YG, "Production of valienamine by a newly isolated strain: Stenotrophomonas maltrophilia", Enzyme and Microbial Technology, 39, pp.1060~1065 (2006)